Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype
Bovine noroviruses are enteric pathogens detected in fecal samples of both diarrheic and non-diarrheic calves from several countries worldwide. However, epidemiological information regarding bovine noroviruses is still lacking for many important cattle producing countries from South America. In this...
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| Format: | Artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1219 http://www.sciencedirect.com/science/article/pii/S156713481630065X https://doi.org/10.1016/j.meegid.2016.02.034 |
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| author | Ferragut, Fátima Vega, Celina Guadalupe Mauroy, Axel Conceição-Neto, Nádia Zeller, Mark Heylen, Elisabeth Louge Uriarte, Enrique Leopoldo Bilbao, Gladys Bok, Marina Matthijnssens, Jelle Thiry, Etienne Badaracco, Alejandra Parreño, Gladys Viviana |
| author_browse | Badaracco, Alejandra Bilbao, Gladys Bok, Marina Conceição-Neto, Nádia Ferragut, Fátima Heylen, Elisabeth Louge Uriarte, Enrique Leopoldo Matthijnssens, Jelle Mauroy, Axel Parreño, Gladys Viviana Thiry, Etienne Vega, Celina Guadalupe Zeller, Mark |
| author_facet | Ferragut, Fátima Vega, Celina Guadalupe Mauroy, Axel Conceição-Neto, Nádia Zeller, Mark Heylen, Elisabeth Louge Uriarte, Enrique Leopoldo Bilbao, Gladys Bok, Marina Matthijnssens, Jelle Thiry, Etienne Badaracco, Alejandra Parreño, Gladys Viviana |
| author_sort | Ferragut, Fátima |
| collection | INTA Digital |
| description | Bovine noroviruses are enteric pathogens detected in fecal samples of both diarrheic and non-diarrheic calves from several countries worldwide. However, epidemiological information regarding bovine noroviruses is still lacking for many important cattle producing countries from South America. In this study, three bovine norovirus genogroup III sequences were determined by conventional RT-PCR and Sanger sequencing in feces from diarrheic
dairy calves from Argentina (B4836, B4848, and B4881, all collected in 2012). Phylogenetic studies based on a partial coding region for the RNA-dependent RNA polymerase (RdRp, 503 nucleotides) of these three samples suggested that two of them (B4836 and B4881) belong to genotype 2 (GIII.2) while the third one (B4848) was more closely related to genotype 1 (GIII.1) strains. By deep sequencing, the capsid region from two of these strains could be determined. This confirmed the circulation of genotype 1 (B4848) together with the presence of another sequence (B4881) sharing its highest genetic relatedness with genotype 1, but sufficiently distant to constitute a new genotype. This latter strain was shown in silico to be a recombinant: phylogenetic divergence was detected between its RNA-dependent RNA polymerase coding sequence (genotype GIII.2) and its capsid protein coding sequence (genotype GIII.1 or a potential norovirus genotype). According to this data, this strain could be the second genotype GIII.2_GIII.1 bovine norovirus recombinant described in literature worldwide. Further analysis suggested that this strain could even be a potential norovirus GIII genotype, tentatively named GIII.4. The data provides important epidemiological and evolutionary information on bovine noroviruses circulating
in South America |
| format | Artículo |
| id | INTA1219 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA12192019-04-05T12:12:48Z Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype Ferragut, Fátima Vega, Celina Guadalupe Mauroy, Axel Conceição-Neto, Nádia Zeller, Mark Heylen, Elisabeth Louge Uriarte, Enrique Leopoldo Bilbao, Gladys Bok, Marina Matthijnssens, Jelle Thiry, Etienne Badaracco, Alejandra Parreño, Gladys Viviana Enfermedades de los Animales Genotipos Virus Ganado de Leche Animal Diseases Genotypes Viruses Dairy Cattle Norovirus Bovine noroviruses are enteric pathogens detected in fecal samples of both diarrheic and non-diarrheic calves from several countries worldwide. However, epidemiological information regarding bovine noroviruses is still lacking for many important cattle producing countries from South America. In this study, three bovine norovirus genogroup III sequences were determined by conventional RT-PCR and Sanger sequencing in feces from diarrheic dairy calves from Argentina (B4836, B4848, and B4881, all collected in 2012). Phylogenetic studies based on a partial coding region for the RNA-dependent RNA polymerase (RdRp, 503 nucleotides) of these three samples suggested that two of them (B4836 and B4881) belong to genotype 2 (GIII.2) while the third one (B4848) was more closely related to genotype 1 (GIII.1) strains. By deep sequencing, the capsid region from two of these strains could be determined. This confirmed the circulation of genotype 1 (B4848) together with the presence of another sequence (B4881) sharing its highest genetic relatedness with genotype 1, but sufficiently distant to constitute a new genotype. This latter strain was shown in silico to be a recombinant: phylogenetic divergence was detected between its RNA-dependent RNA polymerase coding sequence (genotype GIII.2) and its capsid protein coding sequence (genotype GIII.1 or a potential norovirus genotype). According to this data, this strain could be the second genotype GIII.2_GIII.1 bovine norovirus recombinant described in literature worldwide. Further analysis suggested that this strain could even be a potential norovirus GIII genotype, tentatively named GIII.4. The data provides important epidemiological and evolutionary information on bovine noroviruses circulating in South America Inst.de Virología Fil: Ferragut, Fátima. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Mauroy, Axel. University of Liège. Faculty of Veterinary Medicine. Fundamental and Applied Research for Animal and Health Centre. Department of Infectious and Parasitic Diseases. Veterinary Virology and Animal Viral Diseases; Bélgica Fil: Conceição-Neto, Nádia. University of Leuven. Department of Microbiology and Immunology; Bélgica. Rega Institute for Medical Research, Laboratory of Viral Metagenomics; Bélgica Fil: Zeller, Mark. University of Leuven. Department of Microbiology and Immunology. Rega Institute for Medical Research, Laboratory of Viral Metagenomics; Bélgica Fil: Heylen, Elisabeth. University of Leuven. Department of Microbiology and Immunology. Rega Institute for Medical Research, Laboratory of Viral Metagenomics; Bélgica Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Area de Producción Animal; Argentina Fil: Bilbao, Gladys. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela de Veterinaria; Argentina Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Matthijnssens, Jelle. University of Leuven. Department of Microbiology and Immunology; Bélgica. Rega Institute for Medical Research. Laboratory of Viral Metagenomics; Bélgica. Fil: Thiry, Etienne. University of Liège. Faculty of Veterinary Medicine. Fundamental and Applied Research for Animal and Health Centre. Department of Infectious and Parasitic Diseases. Veterinary Virology and Animal Viral Diseases; Bélgica Fil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina 2017-09-14T13:59:44Z 2017-09-14T13:59:44Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1219 http://www.sciencedirect.com/science/article/pii/S156713481630065X 1567-1348 (Print) 1567-7257 (Online) https://doi.org/10.1016/j.meegid.2016.02.034 eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) Infection, genetics and evolution 40 : 144-150. (June 2016) |
| spellingShingle | Enfermedades de los Animales Genotipos Virus Ganado de Leche Animal Diseases Genotypes Viruses Dairy Cattle Norovirus Ferragut, Fátima Vega, Celina Guadalupe Mauroy, Axel Conceição-Neto, Nádia Zeller, Mark Heylen, Elisabeth Louge Uriarte, Enrique Leopoldo Bilbao, Gladys Bok, Marina Matthijnssens, Jelle Thiry, Etienne Badaracco, Alejandra Parreño, Gladys Viviana Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype |
| title | Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype |
| title_full | Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype |
| title_fullStr | Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype |
| title_full_unstemmed | Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype |
| title_short | Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype |
| title_sort | molecular detection of bovine noroviruses in argentinean dairy calves circulation of a tentative new genotype |
| topic | Enfermedades de los Animales Genotipos Virus Ganado de Leche Animal Diseases Genotypes Viruses Dairy Cattle Norovirus |
| url | http://hdl.handle.net/20.500.12123/1219 http://www.sciencedirect.com/science/article/pii/S156713481630065X https://doi.org/10.1016/j.meegid.2016.02.034 |
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