Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo
Retroviruses are not expected to encode miRNAs because of the potential problem of self-cleavage of their genomic RNAs. This assumption has recently been challenged by experiments showing that bovine leukemia virus (BLV) encodes miRNAs from intragenomic Pol III promoters. The BLV miRNAs are abundant...
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| Format: | info:eu-repo/semantics/article |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1218 http://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1005588&type=printable https://doi.org/10.1371/journal.ppat.1005588 |
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| author | Gillet, Nicolas A. Hamaidia, Malik Brogniez, Alix de Gutierrez, Gerónimo Renotte, Nathalie Reichert, Michal Trono, Karina Gabriela Willems, Luc |
| author_browse | Brogniez, Alix de Gillet, Nicolas A. Gutierrez, Gerónimo Hamaidia, Malik Reichert, Michal Renotte, Nathalie Trono, Karina Gabriela Willems, Luc |
| author_facet | Gillet, Nicolas A. Hamaidia, Malik Brogniez, Alix de Gutierrez, Gerónimo Renotte, Nathalie Reichert, Michal Trono, Karina Gabriela Willems, Luc |
| author_sort | Gillet, Nicolas A. |
| collection | INTA Digital |
| description | Retroviruses are not expected to encode miRNAs because of the potential problem of self-cleavage of their genomic RNAs. This assumption has recently been challenged by experiments showing that bovine leukemia virus (BLV) encodes miRNAs from intragenomic Pol III promoters. The BLV miRNAs are abundantly expressed in B-cell tumors in the absence of significant levels of genomic and subgenomic viral RNAs. Using deep RNA sequencing and functional reporter assays, we show that miRNAs mediate the expression of genes involved in cell signaling, cancer and immunity. We further demonstrate that BLV miRNAs are essential to induce B-cell tumors in an experimental model and to promote efficient viral replication in the natural host. |
| format | info:eu-repo/semantics/article |
| id | INTA1218 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA12182018-02-14T18:46:34Z Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo Gillet, Nicolas A. Hamaidia, Malik Brogniez, Alix de Gutierrez, Gerónimo Renotte, Nathalie Reichert, Michal Trono, Karina Gabriela Willems, Luc Enfermedades de los Animales Virus Leucemia Bovina ARN Oncogenicidad Animal Diseases Bovine Leukaemia Virus RNA Oncogenicity Retroviruses are not expected to encode miRNAs because of the potential problem of self-cleavage of their genomic RNAs. This assumption has recently been challenged by experiments showing that bovine leukemia virus (BLV) encodes miRNAs from intragenomic Pol III promoters. The BLV miRNAs are abundantly expressed in B-cell tumors in the absence of significant levels of genomic and subgenomic viral RNAs. Using deep RNA sequencing and functional reporter assays, we show that miRNAs mediate the expression of genes involved in cell signaling, cancer and immunity. We further demonstrate that BLV miRNAs are essential to induce B-cell tumors in an experimental model and to promote efficient viral replication in the natural host. Inst.de Virología Fil: Gillet, Nicolas A. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Bélgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Bélgica Fil: Hamaidia, Malik. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Bélgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Bélgica Fil: Renotte, Nathalie. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Bélgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Bélgica Fil: Reichert, Michal. National Veterinary Research Institute; Polonia Fil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Willems, Luc. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Bélgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Bélgica 2017-09-14T13:41:45Z 2017-09-14T13:41:45Z 2016-04 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1218 http://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1005588&type=printable Gillet NA, Hamaidia M, de Brogniez A, Gutiérrez G, Renotte N, Reichert M, et al. (2016) Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo. PLoS Pathog 12(4): e1005588 1553-7366 (Print) 1553-7374 (Online) https://doi.org/10.1371/journal.ppat.1005588 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf PLOS Pathogens 12(4) : e1005588 (2016) |
| spellingShingle | Enfermedades de los Animales Virus Leucemia Bovina ARN Oncogenicidad Animal Diseases Bovine Leukaemia Virus RNA Oncogenicity Gillet, Nicolas A. Hamaidia, Malik Brogniez, Alix de Gutierrez, Gerónimo Renotte, Nathalie Reichert, Michal Trono, Karina Gabriela Willems, Luc Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo |
| title | Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo |
| title_full | Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo |
| title_fullStr | Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo |
| title_full_unstemmed | Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo |
| title_short | Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo |
| title_sort | bovine leukemia virus small noncoding rnas are functional elements that regulate replication and contribute to oncogenesis in vivo |
| topic | Enfermedades de los Animales Virus Leucemia Bovina ARN Oncogenicidad Animal Diseases Bovine Leukaemia Virus RNA Oncogenicity |
| url | http://hdl.handle.net/20.500.12123/1218 http://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1005588&type=printable https://doi.org/10.1371/journal.ppat.1005588 |
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