Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture = Variación somaclonal en banana evaluada por los perfiles de polimorfismo en la longitud de los fragmentos amplificados en ciclos tempranos de cultivo in vitro
Banana micropropagation for obtaining free-virus plants frequently provokes somaclonal variation that could increase useful genetic variability in this asexually propagated crop. Both exploring the cycle of in vitro culture in which somaclonal variation occurs and the amount...
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| Format: | Artículo |
| Language: | Inglés |
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Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/12139 https://revistas.unc.edu.ar/index.php/agris/article/view/33260 https://doi.org/10.31047/1668.298x.v38.n2.33260 |
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| author | Ermini, José Luis Tenaglia, Gerardo Carlos Parisod, C. Pratta, Guillermo Raúl |
| author_browse | Ermini, José Luis Parisod, C. Pratta, Guillermo Raúl Tenaglia, Gerardo Carlos |
| author_facet | Ermini, José Luis Tenaglia, Gerardo Carlos Parisod, C. Pratta, Guillermo Raúl |
| author_sort | Ermini, José Luis |
| collection | INTA Digital |
| description | Banana micropropagation for obtaining free-virus plants frequently provokes somaclonal variation that could increase useful genetic variability in this asexually propagated crop. Both exploring the cycle of in vitro culture in which somaclonal variation occurs and the amount of generated polymorphism, are necessary. In this work, preliminary results of somaclonal variation during early cycles of banana in vitro culture are reported. Four randomly selected regenerated plants from the fifth cycle and two samples from the mother plant were analyzed. A total of 36 AFLP primer combinations were assayed, and 24 of them produced amplicons varying among 50-500 bp. The mother plant presented a total of 125 different amplicons while the regenerated plants jointly showed 131 different amplicons with a mean of 119.75 ± 3.97 per individual. High level of DNA polymorphism (24.43 %) was found among micropropagated plants and, additionally, the occurrence of somaclonal variation at earlier cycles was suggested by multivariate analysis of Principal Coordinates. In this study, somaclonal variation at early cycles of banana micropropagation was validated and the adequacy of AFLP technique to assess it at the molecular level was verified. The phenotypic effects of the detected somaclonal variations remain to be evaluated. |
| format | Artículo |
| id | INTA12139 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba |
| publisherStr | Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba |
| record_format | dspace |
| spelling | INTA121392024-01-10T11:13:07Z Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture = Variación somaclonal en banana evaluada por los perfiles de polimorfismo en la longitud de los fragmentos amplificados en ciclos tempranos de cultivo in vitro Ermini, José Luis Tenaglia, Gerardo Carlos Parisod, C. Pratta, Guillermo Raúl Banano Variación Somaclonal Micropropagación Polimorfismo de Longitud de Fragmentos Amplificados Variación Genética Cultivo in Vitro Bananas Somaclonal Variation Micropropagation Amplified Fragment Length Polymorphism Genetic Variation In Vitro Culture AFLP Banana micropropagation for obtaining free-virus plants frequently provokes somaclonal variation that could increase useful genetic variability in this asexually propagated crop. Both exploring the cycle of in vitro culture in which somaclonal variation occurs and the amount of generated polymorphism, are necessary. In this work, preliminary results of somaclonal variation during early cycles of banana in vitro culture are reported. Four randomly selected regenerated plants from the fifth cycle and two samples from the mother plant were analyzed. A total of 36 AFLP primer combinations were assayed, and 24 of them produced amplicons varying among 50-500 bp. The mother plant presented a total of 125 different amplicons while the regenerated plants jointly showed 131 different amplicons with a mean of 119.75 ± 3.97 per individual. High level of DNA polymorphism (24.43 %) was found among micropropagated plants and, additionally, the occurrence of somaclonal variation at earlier cycles was suggested by multivariate analysis of Principal Coordinates. In this study, somaclonal variation at early cycles of banana micropropagation was validated and the adequacy of AFLP technique to assess it at the molecular level was verified. The phenotypic effects of the detected somaclonal variations remain to be evaluated. La micropropagación de banana para obtener plantas libres de virus frecuentemente provoca variación somaclonal que puede incrementar la variabilidad genética en cultivos de reproducción asexual. Es necesario explorar el ciclo del cultivo in vitro en que se produce esta variación así como cuantificar el porcentaje de polimorfismo. Este trabajo presenta resultados de variación somaclonal en ciclos tempranos de micropropagación de banana. Cuatro plantas tomadas al azar del quinto ciclo de regeneración y dos muestras de la planta madre se caracterizaron con 36 combinaciones de cebadores de AFLP. Veinticuatro combinaciones produjeron amplicones en un rango entre 50-500 pb. La planta madre presentó en total 125 amplicones mientras que en conjunto las plantas regeneradas mostraron 131 amplicones, con una media de 119,75 ± 3,97 por individuo. Se detectó un alto porcentaje de polimorfismo (24,43 %) en las plantas micropropagadas y, adicionalmente, análisis multivariados de coordenadas principales sugirieron la ocurrencia de variación somaclonal en ciclos de regeneración anteriores. En este estudio se validó la ocurrencia de variación somacloanl en ciclos tempranos de la micropropagación en banana y se verificó que la técnica de AFLP es adecuada para evaluarla a nivel molecular. Queda pendiente evaluar los efectos fenotípicos de estas variantes somaclonales. IPAF Región NEA Fil: Ermini, José Luis. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Biología. Cátedra de Genética; Argentina. Fil: Tenaglia, Gerardo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación y Desarrollo Tecnológico para la Agricultura Familiar Región NEA; Argentina Fil: Parisod, C. Universität Bern. Institute für Pflanzenwissenschaften. Ecological Genomics; Suiza Fil: Pratta, Guillermo. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Biología. Cátedra de Genética; Argentina. Fil: Pratta, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina Fil: Pratta, Guillermo. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina 2022-06-22T11:38:11Z 2022-06-22T11:38:11Z 2021-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12139 https://revistas.unc.edu.ar/index.php/agris/article/view/33260 1668-298X https://doi.org/10.31047/1668.298x.v38.n2.33260 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba AgrisScientia 38 (2) : 143-148 (2021) |
| spellingShingle | Banano Variación Somaclonal Micropropagación Polimorfismo de Longitud de Fragmentos Amplificados Variación Genética Cultivo in Vitro Bananas Somaclonal Variation Micropropagation Amplified Fragment Length Polymorphism Genetic Variation In Vitro Culture AFLP Ermini, José Luis Tenaglia, Gerardo Carlos Parisod, C. Pratta, Guillermo Raúl Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture = Variación somaclonal en banana evaluada por los perfiles de polimorfismo en la longitud de los fragmentos amplificados en ciclos tempranos de cultivo in vitro |
| title | Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture = Variación somaclonal en banana evaluada por los perfiles de polimorfismo en la longitud de los fragmentos amplificados en ciclos tempranos de cultivo in vitro |
| title_full | Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture = Variación somaclonal en banana evaluada por los perfiles de polimorfismo en la longitud de los fragmentos amplificados en ciclos tempranos de cultivo in vitro |
| title_fullStr | Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture = Variación somaclonal en banana evaluada por los perfiles de polimorfismo en la longitud de los fragmentos amplificados en ciclos tempranos de cultivo in vitro |
| title_full_unstemmed | Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture = Variación somaclonal en banana evaluada por los perfiles de polimorfismo en la longitud de los fragmentos amplificados en ciclos tempranos de cultivo in vitro |
| title_short | Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture = Variación somaclonal en banana evaluada por los perfiles de polimorfismo en la longitud de los fragmentos amplificados en ciclos tempranos de cultivo in vitro |
| title_sort | banana somaclonal variation assessed by amplified fragment length polymorphism profiles at early cycles of in vitro culture variacion somaclonal en banana evaluada por los perfiles de polimorfismo en la longitud de los fragmentos amplificados en ciclos tempranos de cultivo in vitro |
| topic | Banano Variación Somaclonal Micropropagación Polimorfismo de Longitud de Fragmentos Amplificados Variación Genética Cultivo in Vitro Bananas Somaclonal Variation Micropropagation Amplified Fragment Length Polymorphism Genetic Variation In Vitro Culture AFLP |
| url | http://hdl.handle.net/20.500.12123/12139 https://revistas.unc.edu.ar/index.php/agris/article/view/33260 https://doi.org/10.31047/1668.298x.v38.n2.33260 |
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