New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development
Aim of study: To validate and characterize new microsatellites or Simple Sequence Repeats (SSR) markers, located within genomic transcribed sequences related to growth and plant developmental traits, in Eucalyptus species. Area of study: Eucalyptus species from different Australian origins plante...
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| Format: | Artículo |
| Language: | Inglés |
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Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/12133 https://revistas.inia.es/index.php/fs/article/view/17074 https://doi.org/10.5424/fs/2020293-17074 |
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| author | Acuña, Cintia Vanesa Rivas, Juan Gabriel Aguirre, Natalia Cristina Villalba, Pamela Victoria Martinez, Maria Carolina Garcia, Martin Nahuel Hopp, Horacio Esteban Marcucci Poltri, Susana Noemi |
| author_browse | Acuña, Cintia Vanesa Aguirre, Natalia Cristina Garcia, Martin Nahuel Hopp, Horacio Esteban Marcucci Poltri, Susana Noemi Martinez, Maria Carolina Rivas, Juan Gabriel Villalba, Pamela Victoria |
| author_facet | Acuña, Cintia Vanesa Rivas, Juan Gabriel Aguirre, Natalia Cristina Villalba, Pamela Victoria Martinez, Maria Carolina Garcia, Martin Nahuel Hopp, Horacio Esteban Marcucci Poltri, Susana Noemi |
| author_sort | Acuña, Cintia Vanesa |
| collection | INTA Digital |
| description | Aim of study: To validate and characterize new microsatellites or Simple Sequence Repeats (SSR) markers, located within genomic transcribed sequences related to growth and plant developmental traits, in Eucalyptus species.
Area of study: Eucalyptus species from different Australian origins planted in Argentina.
Materials and methods: In total, 134 SSR in 129 candidate genes (CG-SSR) involved in plant development were selected and physically mapped to the E. grandis reference genome by bioinformatic tools. Experimental validation and polymorphism analysis were performed on 48 individuals from E. grandis and interspecific hybrids (E. grandis x E. camaldulensis; E. grandis x E. tereticornis), E. globulus, E. maidenii, E. dunnii and E. benthamii.
Main results: 131 out of 134 CG-SSR were mapped on the 11 chromosomes of E. grandis reference genome. Most of the 134 analyzed SSR (> 75%) were positively amplified and 39 were polymorphic in at least one species. A search of annotated genes within a 25 kbp up and downstream region of each SSR location retrieved 773 genes of interest.
Research highlights: The new validated and characterized CG-SSR are potentially suitable for comparative QTL mapping, molecular marker-assisted breeding (MAB) and population genetic studies across different species within Symphyomyrtus subgenus. |
| format | Artículo |
| id | INTA12133 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España) |
| publisherStr | Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España) |
| record_format | dspace |
| spelling | INTA121332022-06-22T10:21:51Z New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development Acuña, Cintia Vanesa Rivas, Juan Gabriel Aguirre, Natalia Cristina Villalba, Pamela Victoria Martinez, Maria Carolina Garcia, Martin Nahuel Hopp, Horacio Esteban Marcucci Poltri, Susana Noemi Eucalyptus Microsatélites Crecimiento Microsatellites Growth Genetic Markers Marcadores Genéticos Candidate Genes Plant Development Genes Candidatos Desarrollo de Plantas Aim of study: To validate and characterize new microsatellites or Simple Sequence Repeats (SSR) markers, located within genomic transcribed sequences related to growth and plant developmental traits, in Eucalyptus species. Area of study: Eucalyptus species from different Australian origins planted in Argentina. Materials and methods: In total, 134 SSR in 129 candidate genes (CG-SSR) involved in plant development were selected and physically mapped to the E. grandis reference genome by bioinformatic tools. Experimental validation and polymorphism analysis were performed on 48 individuals from E. grandis and interspecific hybrids (E. grandis x E. camaldulensis; E. grandis x E. tereticornis), E. globulus, E. maidenii, E. dunnii and E. benthamii. Main results: 131 out of 134 CG-SSR were mapped on the 11 chromosomes of E. grandis reference genome. Most of the 134 analyzed SSR (> 75%) were positively amplified and 39 were polymorphic in at least one species. A search of annotated genes within a 25 kbp up and downstream region of each SSR location retrieved 773 genes of interest. Research highlights: The new validated and characterized CG-SSR are potentially suitable for comparative QTL mapping, molecular marker-assisted breeding (MAB) and population genetic studies across different species within Symphyomyrtus subgenus. Instituto de Biotecnología Fil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Acuña, Cintia Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rivas, Juan Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Rivas, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Aguirre, Natalia Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Aguirre, Natalia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Villalba, Pamela Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Villalba, Pamela Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martinez, Maria Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Martinez, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garcia, Martin Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Garcia, Martin Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Marcucci Poltri, Susana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Marcucci Poltri, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2022-06-22T10:17:48Z 2022-06-22T10:17:48Z 2020 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12133 https://revistas.inia.es/index.php/fs/article/view/17074 2171-9845 https://doi.org/10.5424/fs/2020293-17074 eng info:eu-repograntAgreement/INTA/PNFOR-1104064/AR./Aplicación de herramientas moleculares para el uso y la conservación de la diversidad genética forestal. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España) Forest Systems 29 (3) : eSC08 (2020) |
| spellingShingle | Eucalyptus Microsatélites Crecimiento Microsatellites Growth Genetic Markers Marcadores Genéticos Candidate Genes Plant Development Genes Candidatos Desarrollo de Plantas Acuña, Cintia Vanesa Rivas, Juan Gabriel Aguirre, Natalia Cristina Villalba, Pamela Victoria Martinez, Maria Carolina Garcia, Martin Nahuel Hopp, Horacio Esteban Marcucci Poltri, Susana Noemi New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development |
| title | New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development |
| title_full | New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development |
| title_fullStr | New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development |
| title_full_unstemmed | New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development |
| title_short | New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development |
| title_sort | new validated eucalyptus ssr markers located in candidate genes involved in growth and plant development |
| topic | Eucalyptus Microsatélites Crecimiento Microsatellites Growth Genetic Markers Marcadores Genéticos Candidate Genes Plant Development Genes Candidatos Desarrollo de Plantas |
| url | http://hdl.handle.net/20.500.12123/12133 https://revistas.inia.es/index.php/fs/article/view/17074 https://doi.org/10.5424/fs/2020293-17074 |
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