Molecular and serological detection of Babesia bovis- and Babesia bigemina-infection in bovines and water buffaloes raised jointly in an endemic field
Babesia bovis and Babesia bigemina are causative agents of bovine babesiosis, a tick-borne disease of cattle in tropical and subtropical regions. Babesia spp. infection adversely affects cattle health and can be fatal resulting in considerable economic loss worldwide. Under endemic stability conditi...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1213 http://www.sciencedirect.com/science/article/pii/S030440171530114X?via%3Dihub https://doi.org/10.1016/j.vetpar.2015.12.030 |
| _version_ | 1855034766549581824 |
|---|---|
| author | Romero-Salas, Dora Mira, Anabela Mosqueda, Juan García-Vázquez, Zeferino Hidalgo-Ruiz, Mario Ortiz Vela, Noot Aditya Perez de Leóne, Adalberto Angel Florin-Christensen, Monica Schnittger, Leonhard |
| author_browse | Florin-Christensen, Monica García-Vázquez, Zeferino Hidalgo-Ruiz, Mario Mira, Anabela Mosqueda, Juan Ortiz Vela, Noot Aditya Perez de Leóne, Adalberto Angel Romero-Salas, Dora Schnittger, Leonhard |
| author_facet | Romero-Salas, Dora Mira, Anabela Mosqueda, Juan García-Vázquez, Zeferino Hidalgo-Ruiz, Mario Ortiz Vela, Noot Aditya Perez de Leóne, Adalberto Angel Florin-Christensen, Monica Schnittger, Leonhard |
| author_sort | Romero-Salas, Dora |
| collection | INTA Digital |
| description | Babesia bovis and Babesia bigemina are causative agents of bovine babesiosis, a tick-borne disease of cattle in tropical and subtropical regions. Babesia spp. infection adversely affects cattle health and can be fatal resulting in considerable economic loss worldwide. Under endemic stability conditions, herds contain high numbers of chronically infected, asymptomatic carrier animals, in which no parasitemia is detected by microscopic blood smear examination. In addition to bovines, also water buffaloes are infected by both Babesia spp. commonly leading to a subclinical infection. The infection rate (by nPCR) and herd exposure (by IFAT) of bovines and water buffaloes reared under similar field conditions in an area of endemic stability were determined and compared. In order to optimize direct parasite detection, highly sensitive nPCR assays were developed and applied, allowing the detection of as little as 0.1 fg DNA of each Babesia pathogen. Significantly lower percentages (p < 0.001) of seropositive water buffaloes compared to bovines were observed for B. bovis (71.4% vs. 98%) and B. bigemina (85% vs. 100%). Interestingly, in comparison, differences noticed between water buffaloes and bovines were considerably larger with direct parasite detection by nPCR (16.2% vs. 82.3% and 24% vs. 94.1% for B. bovis and B. bigemina, respectively). As expected, bovines subjected to monthly acaricide applications exhibited a significant lower infection rate as determined by nPCR than bovines not subjected to these measures (B. bovis 33.3% vs. 90.7%, p < 0.001; B. bigemina 80% vs. 96.5%, p < 0.001, for treated vs. untreated animals). Interestingly no differences between these groups were observed with respect to seropositivity, suggesting similar rates of parasite exposure (B. bovis 100% vs. 97.7%, p < 0.001; B. bigemina 100% vs. 100%, p < 0.001). Importantly, a significantly higher number of water buffaloes as determined by nPCR were infected when reared jointly with bovines not subjected to tick control than when reared jointly with bovines subjected to tick control (B. bovis 31.6% vs. 9.5%, p < 0.01; B. bigemina 42.1% vs. 9.5%, p < 0.01, for water buffaloes reared with untreated vs. treated bovines) and/or when reared without bovines (B. bovis 31.6% vs. 11.6%, p < 0.01; B. bigemina 42.1% vs. 20%, p < 0.01). An accumulation of seropositivity and a decline of infection rates were observed in older animals, while differences observed with regard to gender may warrant further investigation. In summary, our findings suggest that water buffaloes are much more capable to limit or eliminate Babesia infection, possibly due to a more capable immune defense. Furthermore, an increased Babesia spp. parasite reservoir of bovines seems to increase the infection rate of water buffaloes when both are reared on the same pasture. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1213 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA12132019-01-24T17:49:33Z Molecular and serological detection of Babesia bovis- and Babesia bigemina-infection in bovines and water buffaloes raised jointly in an endemic field Romero-Salas, Dora Mira, Anabela Mosqueda, Juan García-Vázquez, Zeferino Hidalgo-Ruiz, Mario Ortiz Vela, Noot Aditya Perez de Leóne, Adalberto Angel Florin-Christensen, Monica Schnittger, Leonhard Babesia Bovis Babesia Bigemina Bovinae Búfalo de Agua Inmunodiagnóstico Epidemiología Water Buffaloes Immunodiagnosis Epidemiology Babesia bovis and Babesia bigemina are causative agents of bovine babesiosis, a tick-borne disease of cattle in tropical and subtropical regions. Babesia spp. infection adversely affects cattle health and can be fatal resulting in considerable economic loss worldwide. Under endemic stability conditions, herds contain high numbers of chronically infected, asymptomatic carrier animals, in which no parasitemia is detected by microscopic blood smear examination. In addition to bovines, also water buffaloes are infected by both Babesia spp. commonly leading to a subclinical infection. The infection rate (by nPCR) and herd exposure (by IFAT) of bovines and water buffaloes reared under similar field conditions in an area of endemic stability were determined and compared. In order to optimize direct parasite detection, highly sensitive nPCR assays were developed and applied, allowing the detection of as little as 0.1 fg DNA of each Babesia pathogen. Significantly lower percentages (p < 0.001) of seropositive water buffaloes compared to bovines were observed for B. bovis (71.4% vs. 98%) and B. bigemina (85% vs. 100%). Interestingly, in comparison, differences noticed between water buffaloes and bovines were considerably larger with direct parasite detection by nPCR (16.2% vs. 82.3% and 24% vs. 94.1% for B. bovis and B. bigemina, respectively). As expected, bovines subjected to monthly acaricide applications exhibited a significant lower infection rate as determined by nPCR than bovines not subjected to these measures (B. bovis 33.3% vs. 90.7%, p < 0.001; B. bigemina 80% vs. 96.5%, p < 0.001, for treated vs. untreated animals). Interestingly no differences between these groups were observed with respect to seropositivity, suggesting similar rates of parasite exposure (B. bovis 100% vs. 97.7%, p < 0.001; B. bigemina 100% vs. 100%, p < 0.001). Importantly, a significantly higher number of water buffaloes as determined by nPCR were infected when reared jointly with bovines not subjected to tick control than when reared jointly with bovines subjected to tick control (B. bovis 31.6% vs. 9.5%, p < 0.01; B. bigemina 42.1% vs. 9.5%, p < 0.01, for water buffaloes reared with untreated vs. treated bovines) and/or when reared without bovines (B. bovis 31.6% vs. 11.6%, p < 0.01; B. bigemina 42.1% vs. 20%, p < 0.01). An accumulation of seropositivity and a decline of infection rates were observed in older animals, while differences observed with regard to gender may warrant further investigation. In summary, our findings suggest that water buffaloes are much more capable to limit or eliminate Babesia infection, possibly due to a more capable immune defense. Furthermore, an increased Babesia spp. parasite reservoir of bovines seems to increase the infection rate of water buffaloes when both are reared on the same pasture. Fil: Romero-Salas, Dora. Universidad Veracruzana. Facultad de Medicina Veterinaria y Zootecnia. Laboratorio de Parasitología; México Fil: Mira, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Mosqueda, Juan. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México Fil: García-Vázquez, Zeferino. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria; México Fil: Hidalgo-Ruiz, Mario. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México Fil: Ortiz Vela, Noot Aditya. Universidad Veracruzana. Facultad de Medicina Veterinaria y Zootecnia. Laboratorio de Parasitología; México Fil: Perez de Leóne, Adalberto Angel. United States Department of Agriculture. Knipling-Bushland U. S. Livestock Insects Research Laboratory; Estados Unidos Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-09-14T12:00:53Z 2017-09-14T12:00:53Z 2016-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1213 http://www.sciencedirect.com/science/article/pii/S030440171530114X?via%3Dihub 0304-4017 https://doi.org/10.1016/j.vetpar.2015.12.030 eng info:eu-repo/semantics/restrictedAccess application/pdf Veterinary parasitology 217 : 101–107. (2016) |
| spellingShingle | Babesia Bovis Babesia Bigemina Bovinae Búfalo de Agua Inmunodiagnóstico Epidemiología Water Buffaloes Immunodiagnosis Epidemiology Romero-Salas, Dora Mira, Anabela Mosqueda, Juan García-Vázquez, Zeferino Hidalgo-Ruiz, Mario Ortiz Vela, Noot Aditya Perez de Leóne, Adalberto Angel Florin-Christensen, Monica Schnittger, Leonhard Molecular and serological detection of Babesia bovis- and Babesia bigemina-infection in bovines and water buffaloes raised jointly in an endemic field |
| title | Molecular and serological detection of Babesia bovis- and Babesia bigemina-infection in bovines and water buffaloes raised jointly in an endemic field |
| title_full | Molecular and serological detection of Babesia bovis- and Babesia bigemina-infection in bovines and water buffaloes raised jointly in an endemic field |
| title_fullStr | Molecular and serological detection of Babesia bovis- and Babesia bigemina-infection in bovines and water buffaloes raised jointly in an endemic field |
| title_full_unstemmed | Molecular and serological detection of Babesia bovis- and Babesia bigemina-infection in bovines and water buffaloes raised jointly in an endemic field |
| title_short | Molecular and serological detection of Babesia bovis- and Babesia bigemina-infection in bovines and water buffaloes raised jointly in an endemic field |
| title_sort | molecular and serological detection of babesia bovis and babesia bigemina infection in bovines and water buffaloes raised jointly in an endemic field |
| topic | Babesia Bovis Babesia Bigemina Bovinae Búfalo de Agua Inmunodiagnóstico Epidemiología Water Buffaloes Immunodiagnosis Epidemiology |
| url | http://hdl.handle.net/20.500.12123/1213 http://www.sciencedirect.com/science/article/pii/S030440171530114X?via%3Dihub https://doi.org/10.1016/j.vetpar.2015.12.030 |
| work_keys_str_mv | AT romerosalasdora molecularandserologicaldetectionofbabesiabovisandbabesiabigeminainfectioninbovinesandwaterbuffaloesraisedjointlyinanendemicfield AT miraanabela molecularandserologicaldetectionofbabesiabovisandbabesiabigeminainfectioninbovinesandwaterbuffaloesraisedjointlyinanendemicfield AT mosquedajuan molecularandserologicaldetectionofbabesiabovisandbabesiabigeminainfectioninbovinesandwaterbuffaloesraisedjointlyinanendemicfield AT garciavazquezzeferino molecularandserologicaldetectionofbabesiabovisandbabesiabigeminainfectioninbovinesandwaterbuffaloesraisedjointlyinanendemicfield AT hidalgoruizmario molecularandserologicaldetectionofbabesiabovisandbabesiabigeminainfectioninbovinesandwaterbuffaloesraisedjointlyinanendemicfield AT ortizvelanootaditya molecularandserologicaldetectionofbabesiabovisandbabesiabigeminainfectioninbovinesandwaterbuffaloesraisedjointlyinanendemicfield AT perezdeleoneadalbertoangel molecularandserologicaldetectionofbabesiabovisandbabesiabigeminainfectioninbovinesandwaterbuffaloesraisedjointlyinanendemicfield AT florinchristensenmonica molecularandserologicaldetectionofbabesiabovisandbabesiabigeminainfectioninbovinesandwaterbuffaloesraisedjointlyinanendemicfield AT schnittgerleonhard molecularandserologicaldetectionofbabesiabovisandbabesiabigeminainfectioninbovinesandwaterbuffaloesraisedjointlyinanendemicfield |