Expanding the size limit of RNA viruses: Evidence of a novel divergent nidovirus in California sea hare, with a ~35.9 kb virus genome
While RNA viruses thrive with massive structural and functional diversity, their genomes size variation is particularly low, ranging only from ~2-to-33 kb. Here, I present the characterization of RNA sequences corresponding to the first virus associated with Aplysia californica. Genome structure and...
| Autor principal: | |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
BioRxiv
2022
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/12109 https://www.biorxiv.org/content/10.1101/307678v1 https://doi.org/10.1101/307678 |
| _version_ | 1855484910707408896 |
|---|---|
| author | Debat, Humberto Julio |
| author_browse | Debat, Humberto Julio |
| author_facet | Debat, Humberto Julio |
| author_sort | Debat, Humberto Julio |
| collection | INTA Digital |
| description | While RNA viruses thrive with massive structural and functional diversity, their genomes size variation is particularly low, ranging only from ~2-to-33 kb. Here, I present the characterization of RNA sequences corresponding to the first virus associated with Aplysia californica. Genome structure and domain architecture suggest that the identified virus is a novel member of Nidovirales. The proposed aplysia californica nido-like virus (AcNV), with a genome sequence of ca.35,906 nt, represents the longest ever recorded RNA virus yet. Phylogenetic insights indicate that AcNV clusters in a major phylloclade of unclassified invertebrate nidoviruses, Roniviridae, and Mesoniviridae. Basal branching in this emerging cluster could indicate that AcNV is a member of a novel divergent clade within Nidovirales. Further, virus RNA detection in multiple independent studies suggests that AcNV is neurotropic with a broad cell/tissue/organ tropism, supported by AcNV occurrence in diverse organs, including the first detection of a Nidovirales in single specific neurons. |
| format | Artículo |
| id | INTA12109 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | BioRxiv |
| publisherStr | BioRxiv |
| record_format | dspace |
| spelling | INTA121092022-06-16T10:50:10Z Expanding the size limit of RNA viruses: Evidence of a novel divergent nidovirus in California sea hare, with a ~35.9 kb virus genome Debat, Humberto Julio Nidovirales RNA Viruses Virología Aplysia Californica Virus Discovery Single-Cell Virology Neurotropic Virus While RNA viruses thrive with massive structural and functional diversity, their genomes size variation is particularly low, ranging only from ~2-to-33 kb. Here, I present the characterization of RNA sequences corresponding to the first virus associated with Aplysia californica. Genome structure and domain architecture suggest that the identified virus is a novel member of Nidovirales. The proposed aplysia californica nido-like virus (AcNV), with a genome sequence of ca.35,906 nt, represents the longest ever recorded RNA virus yet. Phylogenetic insights indicate that AcNV clusters in a major phylloclade of unclassified invertebrate nidoviruses, Roniviridae, and Mesoniviridae. Basal branching in this emerging cluster could indicate that AcNV is a member of a novel divergent clade within Nidovirales. Further, virus RNA detection in multiple independent studies suggests that AcNV is neurotropic with a broad cell/tissue/organ tropism, supported by AcNV occurrence in diverse organs, including the first detection of a Nidovirales in single specific neurons. Instituto de Patología Vegetal Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2022-06-16T10:39:56Z 2022-06-16T10:39:56Z 2018-04-24 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/12109 https://www.biorxiv.org/content/10.1101/307678v1 https://doi.org/10.1101/307678 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BioRxiv BioRxiv : the preprint server for biology (April 24, 2018) |
| spellingShingle | Nidovirales RNA Viruses Virología Aplysia Californica Virus Discovery Single-Cell Virology Neurotropic Virus Debat, Humberto Julio Expanding the size limit of RNA viruses: Evidence of a novel divergent nidovirus in California sea hare, with a ~35.9 kb virus genome |
| title | Expanding the size limit of RNA viruses: Evidence of a novel divergent nidovirus in California sea hare, with a ~35.9 kb virus genome |
| title_full | Expanding the size limit of RNA viruses: Evidence of a novel divergent nidovirus in California sea hare, with a ~35.9 kb virus genome |
| title_fullStr | Expanding the size limit of RNA viruses: Evidence of a novel divergent nidovirus in California sea hare, with a ~35.9 kb virus genome |
| title_full_unstemmed | Expanding the size limit of RNA viruses: Evidence of a novel divergent nidovirus in California sea hare, with a ~35.9 kb virus genome |
| title_short | Expanding the size limit of RNA viruses: Evidence of a novel divergent nidovirus in California sea hare, with a ~35.9 kb virus genome |
| title_sort | expanding the size limit of rna viruses evidence of a novel divergent nidovirus in california sea hare with a 35 9 kb virus genome |
| topic | Nidovirales RNA Viruses Virología Aplysia Californica Virus Discovery Single-Cell Virology Neurotropic Virus |
| url | http://hdl.handle.net/20.500.12123/12109 https://www.biorxiv.org/content/10.1101/307678v1 https://doi.org/10.1101/307678 |
| work_keys_str_mv | AT debathumbertojulio expandingthesizelimitofrnavirusesevidenceofanoveldivergentnidovirusincaliforniaseaharewitha359kbvirusgenome |