Expanding the benefits of Tnt1 for the identification of dominant mutations in polyploid crops: A single allelic mutation in the MsNAC39 gene produces multifoliated alfalfa
Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2022
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/12065 https://www.frontiersin.org/articles/10.3389/fpls.2021.805032/full https://doi.org/10.3389/fpls.2021.805032 |
| _version_ | 1855484902674268160 |
|---|---|
| author | Jozefkowicz, Cintia Gomez, Maria Cristina Odorizzi, Ariel Iantcheva, Anelia Ratet, Pascal Ayub, Nicolás Daniel Soto, Gabriela Cynthia |
| author_browse | Ayub, Nicolás Daniel Gomez, Maria Cristina Iantcheva, Anelia Jozefkowicz, Cintia Odorizzi, Ariel Ratet, Pascal Soto, Gabriela Cynthia |
| author_facet | Jozefkowicz, Cintia Gomez, Maria Cristina Odorizzi, Ariel Iantcheva, Anelia Ratet, Pascal Ayub, Nicolás Daniel Soto, Gabriela Cynthia |
| author_sort | Jozefkowicz, Cintia |
| collection | INTA Digital |
| description | Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we show that the Tnt1 element can be used to identify dominant mutations in allogamous tetraploid cultivated alfalfa. Specifically, we show that a single allelic mutation in the MsNAC39 gene produces multifoliate leaves (mfl) alfalfa plants, a pivot trait of breeding programs of this forage species. Finally, we discuss the potential application of a combination of preliminary screening of beneficial dominant mutants using Tnt1 mutant libraries and genome editing via the CRISPR/Cas9 system to identify target genes and to rapidly improve both autogamous and allogamous polyploid crops. |
| format | Artículo |
| id | INTA12065 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA120652022-06-10T10:52:14Z Expanding the benefits of Tnt1 for the identification of dominant mutations in polyploid crops: A single allelic mutation in the MsNAC39 gene produces multifoliated alfalfa Jozefkowicz, Cintia Gomez, Maria Cristina Odorizzi, Ariel Iantcheva, Anelia Ratet, Pascal Ayub, Nicolás Daniel Soto, Gabriela Cynthia Genetic Transformation Polyploidy Mutation Breeding Gene Editing CRISPR Transformación Genética Poliploidia Medicago sativa Mejoramiento por Mutación Edición de Genes Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we show that the Tnt1 element can be used to identify dominant mutations in allogamous tetraploid cultivated alfalfa. Specifically, we show that a single allelic mutation in the MsNAC39 gene produces multifoliate leaves (mfl) alfalfa plants, a pivot trait of breeding programs of this forage species. Finally, we discuss the potential application of a combination of preliminary screening of beneficial dominant mutants using Tnt1 mutant libraries and genome editing via the CRISPR/Cas9 system to identify target genes and to rapidly improve both autogamous and allogamous polyploid crops. Instituto de Biotecnología Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Jozefkowicz, Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Gomez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Odorizzi, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Iantcheva, Anelia. Agricultural Academy. AgroBioInstitute; Bulgaria Fil: Ratet, Pascal. Centre National de la Recherche Scientifique (CNRS). Institut National de Recherche pour l’agriculture, l’alimentation et l’environnement (INRAE). Université Paris-Saclay; Francia Fil: Ratet, Pascal. Institute of Plant Sciences Paris-Saclay. Université d’Évry; Francia Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Soto, Gabriela Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2022-06-10T10:39:03Z 2022-06-10T10:39:03Z 2022-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12065 https://www.frontiersin.org/articles/10.3389/fpls.2021.805032/full 1664-462X https://doi.org/10.3389/fpls.2021.805032 eng info:eu-repograntAgreement/INTA/2019-PE-E6-I115-001/2019-PE-E6-I115-001/AR./Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuario info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Plant Science 12 : 805032. (December 2021) |
| spellingShingle | Genetic Transformation Polyploidy Mutation Breeding Gene Editing CRISPR Transformación Genética Poliploidia Medicago sativa Mejoramiento por Mutación Edición de Genes Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Jozefkowicz, Cintia Gomez, Maria Cristina Odorizzi, Ariel Iantcheva, Anelia Ratet, Pascal Ayub, Nicolás Daniel Soto, Gabriela Cynthia Expanding the benefits of Tnt1 for the identification of dominant mutations in polyploid crops: A single allelic mutation in the MsNAC39 gene produces multifoliated alfalfa |
| title | Expanding the benefits of Tnt1 for the identification of dominant mutations in polyploid crops: A single allelic mutation in the MsNAC39 gene produces multifoliated alfalfa |
| title_full | Expanding the benefits of Tnt1 for the identification of dominant mutations in polyploid crops: A single allelic mutation in the MsNAC39 gene produces multifoliated alfalfa |
| title_fullStr | Expanding the benefits of Tnt1 for the identification of dominant mutations in polyploid crops: A single allelic mutation in the MsNAC39 gene produces multifoliated alfalfa |
| title_full_unstemmed | Expanding the benefits of Tnt1 for the identification of dominant mutations in polyploid crops: A single allelic mutation in the MsNAC39 gene produces multifoliated alfalfa |
| title_short | Expanding the benefits of Tnt1 for the identification of dominant mutations in polyploid crops: A single allelic mutation in the MsNAC39 gene produces multifoliated alfalfa |
| title_sort | expanding the benefits of tnt1 for the identification of dominant mutations in polyploid crops a single allelic mutation in the msnac39 gene produces multifoliated alfalfa |
| topic | Genetic Transformation Polyploidy Mutation Breeding Gene Editing CRISPR Transformación Genética Poliploidia Medicago sativa Mejoramiento por Mutación Edición de Genes Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas |
| url | http://hdl.handle.net/20.500.12123/12065 https://www.frontiersin.org/articles/10.3389/fpls.2021.805032/full https://doi.org/10.3389/fpls.2021.805032 |
| work_keys_str_mv | AT jozefkowiczcintia expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT gomezmariacristina expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT odorizziariel expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT iantchevaanelia expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT ratetpascal expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT ayubnicolasdaniel expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT sotogabrielacynthia expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa |