Citrus genetic transformation: an overview of the current strategies and insights on the new emerging technologies
Citrus are among the most prevailing fruit crops produced worldwide. The implementation of effective and reliable breeding programs is essential for coping with the increasing demands of satisfactory yield and quality of the fruit as well as to deal with the negative impact of fast-spreading disease...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Frontiers Media
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/12051 https://www.frontiersin.org/articles/10.3389/fpls.2021.768197/full https://doi.org/10.3389/fpls.2021.768197 |
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| author | Conti, Gabriela Xoconostle-Cázares, Beatriz Marcelino-Pérez, Gabriel Hopp, Horacio Esteban Reyes Martinez, Carina Andrea |
| author_browse | Conti, Gabriela Hopp, Horacio Esteban Marcelino-Pérez, Gabriel Reyes Martinez, Carina Andrea Xoconostle-Cázares, Beatriz |
| author_facet | Conti, Gabriela Xoconostle-Cázares, Beatriz Marcelino-Pérez, Gabriel Hopp, Horacio Esteban Reyes Martinez, Carina Andrea |
| author_sort | Conti, Gabriela |
| collection | INTA Digital |
| description | Citrus are among the most prevailing fruit crops produced worldwide. The implementation of effective and reliable breeding programs is essential for coping with the increasing demands of satisfactory yield and quality of the fruit as well as to deal with the negative impact of fast-spreading diseases. Conventional methods are time-consuming and of difficult application because of inherent factors of citrus biology, such as their prolonged juvenile period and a complex reproductive stage, sometimes presenting infertility, self-incompatibility, parthenocarpy, or polyembryony. Moreover, certain desirable traits are absent from cultivated or wild citrus genotypes. All these features are challenging for the incorporation of the desirable traits. In this regard, genetic engineering technologies offer a series of alternative approaches that allow overcoming the difficulties of conventional breeding programs. This review gives a detailed overview of the currently used strategies for the development of genetically modified citrus. We describe different aspects regarding genotype varieties used, including elite cultivars or extensively used scions and rootstocks. Furthermore, we discuss technical aspects of citrus genetic transformation procedures via Agrobacterium, regular physical methods, and magnetofection. Finally, we describe the selection of explants considering young and mature tissues, protoplast isolation, etc. We also address current protocols and novel approaches for improving the in vitro regeneration process, which is an important bottleneck for citrus genetic transformation. This review also explores alternative emerging transformation strategies applied to citrus species such as transient and tissue localized transformation. New breeding technologies, including cisgenesis, intragenesis, and genome editing by clustered regularly interspaced short palindromic repeats (CRISPR), are also discussed. Other relevant aspects comprising new promoters and reporter genes, marker-free systems, and strategies for induction of early flowering, are also addressed. We provided a future perspective on the use of current and new technologies in citrus and its potential impact on regulatory processes. |
| format | Artículo |
| id | INTA12051 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA120512022-06-09T10:22:09Z Citrus genetic transformation: an overview of the current strategies and insights on the new emerging technologies Conti, Gabriela Xoconostle-Cázares, Beatriz Marcelino-Pérez, Gabriel Hopp, Horacio Esteban Reyes Martinez, Carina Andrea Citrus Plantas Transgénicas Regeneración in Vitro Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Biotecnología Genes Indicadores Tecnología Nueva Transgenic Plants In Vitro Regeneration CRISPR Biotechnology Reporter Genes New Technology Cisgenesis Intragenesis Cisgénesis Intragénesis Citrus are among the most prevailing fruit crops produced worldwide. The implementation of effective and reliable breeding programs is essential for coping with the increasing demands of satisfactory yield and quality of the fruit as well as to deal with the negative impact of fast-spreading diseases. Conventional methods are time-consuming and of difficult application because of inherent factors of citrus biology, such as their prolonged juvenile period and a complex reproductive stage, sometimes presenting infertility, self-incompatibility, parthenocarpy, or polyembryony. Moreover, certain desirable traits are absent from cultivated or wild citrus genotypes. All these features are challenging for the incorporation of the desirable traits. In this regard, genetic engineering technologies offer a series of alternative approaches that allow overcoming the difficulties of conventional breeding programs. This review gives a detailed overview of the currently used strategies for the development of genetically modified citrus. We describe different aspects regarding genotype varieties used, including elite cultivars or extensively used scions and rootstocks. Furthermore, we discuss technical aspects of citrus genetic transformation procedures via Agrobacterium, regular physical methods, and magnetofection. Finally, we describe the selection of explants considering young and mature tissues, protoplast isolation, etc. We also address current protocols and novel approaches for improving the in vitro regeneration process, which is an important bottleneck for citrus genetic transformation. This review also explores alternative emerging transformation strategies applied to citrus species such as transient and tissue localized transformation. New breeding technologies, including cisgenesis, intragenesis, and genome editing by clustered regularly interspaced short palindromic repeats (CRISPR), are also discussed. Other relevant aspects comprising new promoters and reporter genes, marker-free systems, and strategies for induction of early flowering, are also addressed. We provided a future perspective on the use of current and new technologies in citrus and its potential impact on regulatory processes. Instituto de Biotecnología Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Conti, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Conti, Gabriela. Universidad de Buenos Aires. Faculta de Agronomía. Cátedra de Genética; Argentina Fil: Xoconostle-Cázares, Beatriz. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional. Departamento de Biotecnología y Bioingeniería; México Fil: Marcelino-Pérez, Gabriel. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional. Departamento de Biotecnología y Bioingeniería; México Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Reyes Martinez, Carina Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Reyes Martinez, Carina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina 2022-06-09T10:16:54Z 2022-06-09T10:16:54Z 2021-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12051 https://www.frontiersin.org/articles/10.3389/fpls.2021.768197/full 1664-462X https://doi.org/10.3389/fpls.2021.768197 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Plant Science 12 : 768197 (Noviembre 2021) |
| spellingShingle | Citrus Plantas Transgénicas Regeneración in Vitro Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Biotecnología Genes Indicadores Tecnología Nueva Transgenic Plants In Vitro Regeneration CRISPR Biotechnology Reporter Genes New Technology Cisgenesis Intragenesis Cisgénesis Intragénesis Conti, Gabriela Xoconostle-Cázares, Beatriz Marcelino-Pérez, Gabriel Hopp, Horacio Esteban Reyes Martinez, Carina Andrea Citrus genetic transformation: an overview of the current strategies and insights on the new emerging technologies |
| title | Citrus genetic transformation: an overview of the current strategies and insights on the new emerging technologies |
| title_full | Citrus genetic transformation: an overview of the current strategies and insights on the new emerging technologies |
| title_fullStr | Citrus genetic transformation: an overview of the current strategies and insights on the new emerging technologies |
| title_full_unstemmed | Citrus genetic transformation: an overview of the current strategies and insights on the new emerging technologies |
| title_short | Citrus genetic transformation: an overview of the current strategies and insights on the new emerging technologies |
| title_sort | citrus genetic transformation an overview of the current strategies and insights on the new emerging technologies |
| topic | Citrus Plantas Transgénicas Regeneración in Vitro Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Biotecnología Genes Indicadores Tecnología Nueva Transgenic Plants In Vitro Regeneration CRISPR Biotechnology Reporter Genes New Technology Cisgenesis Intragenesis Cisgénesis Intragénesis |
| url | http://hdl.handle.net/20.500.12123/12051 https://www.frontiersin.org/articles/10.3389/fpls.2021.768197/full https://doi.org/10.3389/fpls.2021.768197 |
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