State of the art of genetic engineering in potato: from the first report to its future potential
Potato (Solanum tuberosum L.) is a crop of world importance that produces tubers of high nutritional quality. It is considered one of the promising crops to overcome the challenges of poverty and hunger worldwide. However, it is exposed to different biotic and abiotic stresses that can cause signifi...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/12050 https://www.frontiersin.org/articles/10.3389/fpls.2021.768233/full https://doi.org/10.3389/fpls.2021.768233 |
| _version_ | 1855036685339852800 |
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| author | Nahirñak, Vanesa Almasia, Natalia Ines Gonzalez, Matías Nicolás Massa, Gabriela Alejandra Decima Oneto, Cecilia Andrea Feingold, Sergio Enrique Hopp, Horacio Esteban Vazquez Rovere, Cecilia |
| author_browse | Almasia, Natalia Ines Decima Oneto, Cecilia Andrea Feingold, Sergio Enrique Gonzalez, Matías Nicolás Hopp, Horacio Esteban Massa, Gabriela Alejandra Nahirñak, Vanesa Vazquez Rovere, Cecilia |
| author_facet | Nahirñak, Vanesa Almasia, Natalia Ines Gonzalez, Matías Nicolás Massa, Gabriela Alejandra Decima Oneto, Cecilia Andrea Feingold, Sergio Enrique Hopp, Horacio Esteban Vazquez Rovere, Cecilia |
| author_sort | Nahirñak, Vanesa |
| collection | INTA Digital |
| description | Potato (Solanum tuberosum L.) is a crop of world importance that produces tubers of high nutritional quality. It is considered one of the promising crops to overcome the challenges of poverty and hunger worldwide. However, it is exposed to different biotic and abiotic stresses that can cause significant losses in production. Thus, potato is a candidate of special relevance for improvements through conventional breeding and biotechnology. Since conventional breeding is time-consuming and challenging, genetic engineering provides the opportunity to introduce/switch-off genes of interest without altering the allelic combination that characterize successful commercial cultivars or to induce targeted sequence modifications by New Breeding Techniques. There is a variety of methods for potato improvement via genetic transformation. Most of them incorporate genes of interest into the nuclear genome; nevertheless, the development of plastid transformation protocols broadened the available approaches for potato breeding. Although all methods have their advantages and disadvantages, Agrobacterium-mediated transformation is the most used approach. Alternative methods such as particle bombardment, protoplast transfection with polyethylene glycol and microinjection are also effective. Independently of the DNA delivery approach, critical steps for a successful transformation are a rapid and efficient regeneration protocol and a selection system. Several critical factors affect the transformation efficiency: vector type, insert size, Agrobacterium strain, explant type, composition of the subculture media, selective agent, among others. Moreover, transient or stable transformation, constitutive or inducible promoters, antibiotic/herbicide resistance or marker-free strategies can be considered. Although great efforts have been made to optimize all the parameters, potato transformation protocols are highly genotype-dependent. Genome editing technologies provide promising tools in genetic engineering allowing precise modification of targeted sequences. Interestingly, transient expression of genome editing components in potato protoplasts was reported to generate edited plants without the integration of any foreign DNA, which is a valuable aspect from both a scientific and a regulatory perspective. In this review, current challenges and opportunities concerning potato genetic engineering strategies developed to date are discussed. We describe their critical parameters and constrains, and the potential application of the available tools for functional analyses or biotechnological purposes. Public concerns and safety issues are also addressed. |
| format | info:ar-repo/semantics/artículo |
| id | INTA12050 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA120502022-06-09T10:12:00Z State of the art of genetic engineering in potato: from the first report to its future potential Nahirñak, Vanesa Almasia, Natalia Ines Gonzalez, Matías Nicolás Massa, Gabriela Alejandra Decima Oneto, Cecilia Andrea Feingold, Sergio Enrique Hopp, Horacio Esteban Vazquez Rovere, Cecilia Potatoes Genetic Engineering Biotechnology Agrobacterium New Technology Gene Editing Papa Ingeniería Genética Biotecnología Solanum tuberosum Tecnología Nueva Edición de Genes Potato (Solanum tuberosum L.) is a crop of world importance that produces tubers of high nutritional quality. It is considered one of the promising crops to overcome the challenges of poverty and hunger worldwide. However, it is exposed to different biotic and abiotic stresses that can cause significant losses in production. Thus, potato is a candidate of special relevance for improvements through conventional breeding and biotechnology. Since conventional breeding is time-consuming and challenging, genetic engineering provides the opportunity to introduce/switch-off genes of interest without altering the allelic combination that characterize successful commercial cultivars or to induce targeted sequence modifications by New Breeding Techniques. There is a variety of methods for potato improvement via genetic transformation. Most of them incorporate genes of interest into the nuclear genome; nevertheless, the development of plastid transformation protocols broadened the available approaches for potato breeding. Although all methods have their advantages and disadvantages, Agrobacterium-mediated transformation is the most used approach. Alternative methods such as particle bombardment, protoplast transfection with polyethylene glycol and microinjection are also effective. Independently of the DNA delivery approach, critical steps for a successful transformation are a rapid and efficient regeneration protocol and a selection system. Several critical factors affect the transformation efficiency: vector type, insert size, Agrobacterium strain, explant type, composition of the subculture media, selective agent, among others. Moreover, transient or stable transformation, constitutive or inducible promoters, antibiotic/herbicide resistance or marker-free strategies can be considered. Although great efforts have been made to optimize all the parameters, potato transformation protocols are highly genotype-dependent. Genome editing technologies provide promising tools in genetic engineering allowing precise modification of targeted sequences. Interestingly, transient expression of genome editing components in potato protoplasts was reported to generate edited plants without the integration of any foreign DNA, which is a valuable aspect from both a scientific and a regulatory perspective. In this review, current challenges and opportunities concerning potato genetic engineering strategies developed to date are discussed. We describe their critical parameters and constrains, and the potential application of the available tools for functional analyses or biotechnological purposes. Public concerns and safety issues are also addressed. Instituto de Biotecnología Fil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Nahirñak, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Almasia, Natalia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: González, Matías Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: González, Matías Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Massa, Gabriela Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Massa, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Massa, Gabriela Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Décima Oneto, Cecilia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Décima Oneto, Cecilia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Décima Oneto, Cecilia Andrea. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Feingold, Sergio Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Feingold, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Vazquez Rovere, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2022-06-09T10:05:29Z 2022-06-09T10:05:29Z 2022-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12050 https://www.frontiersin.org/articles/10.3389/fpls.2021.768233/full 1664-462X https://doi.org/10.3389/fpls.2021.768233 eng info:eu-repograntAgreement/INTA/2019-PE-E6-I509-001/2019-PE-E6-I509-001/AR./Mejoramiento genético de especies hortícolas de uso semi extensivo: PAPA y BATATA info:eu-repograntAgreement/INTA/2019-PE-E6-I115-001/2019-PE-E6-I115-001/AR./Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuario info:eu-repograntAgreement/INTA/2019-PD-E4-I085-001/2019-PD-E4-I085-001/AR./Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno. info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001/2019-PD-E6-I116-001/AR./Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Plant Science 12 : 768233 (Enero 2022) |
| spellingShingle | Potatoes Genetic Engineering Biotechnology Agrobacterium New Technology Gene Editing Papa Ingeniería Genética Biotecnología Solanum tuberosum Tecnología Nueva Edición de Genes Nahirñak, Vanesa Almasia, Natalia Ines Gonzalez, Matías Nicolás Massa, Gabriela Alejandra Decima Oneto, Cecilia Andrea Feingold, Sergio Enrique Hopp, Horacio Esteban Vazquez Rovere, Cecilia State of the art of genetic engineering in potato: from the first report to its future potential |
| title | State of the art of genetic engineering in potato: from the first report to its future potential |
| title_full | State of the art of genetic engineering in potato: from the first report to its future potential |
| title_fullStr | State of the art of genetic engineering in potato: from the first report to its future potential |
| title_full_unstemmed | State of the art of genetic engineering in potato: from the first report to its future potential |
| title_short | State of the art of genetic engineering in potato: from the first report to its future potential |
| title_sort | state of the art of genetic engineering in potato from the first report to its future potential |
| topic | Potatoes Genetic Engineering Biotechnology Agrobacterium New Technology Gene Editing Papa Ingeniería Genética Biotecnología Solanum tuberosum Tecnología Nueva Edición de Genes |
| url | http://hdl.handle.net/20.500.12123/12050 https://www.frontiersin.org/articles/10.3389/fpls.2021.768233/full https://doi.org/10.3389/fpls.2021.768233 |
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