Genetic and Transcription Profile Analysis of Tissue-Specific Anthocyanin Pigmentation in Carrot Root Phloem
In purple carrots, anthocyanin pigmentation can be expressed in the entire root, or it can display tissue specific-patterns. Within the phloem, purple pigmentation can be found in the outer phloem (OP) (also called the cortex) and inner phloem (IP), or it can be confined exclusively to the OP. In th...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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MDPI
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/12033 https://www.mdpi.com/2073-4425/12/10/1464 https://doi.org/10.3390/genes12101464 |
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| author | Bannoud, Florencia Carvajal, Sofía Ellison, Shelby L. Senalik, Douglas A. Gomez Talquenca, Gonzalo Iorizzo, Massimo Simon, Philipp W. Cavagnaro, Pablo |
| author_browse | Bannoud, Florencia Carvajal, Sofía Cavagnaro, Pablo Ellison, Shelby L. Gomez Talquenca, Gonzalo Iorizzo, Massimo Senalik, Douglas A. Simon, Philipp W. |
| author_facet | Bannoud, Florencia Carvajal, Sofía Ellison, Shelby L. Senalik, Douglas A. Gomez Talquenca, Gonzalo Iorizzo, Massimo Simon, Philipp W. Cavagnaro, Pablo |
| author_sort | Bannoud, Florencia |
| collection | INTA Digital |
| description | In purple carrots, anthocyanin pigmentation can be expressed in the entire root, or it can display tissue specific-patterns. Within the phloem, purple pigmentation can be found in the outer phloem (OP) (also called the cortex) and inner phloem (IP), or it can be confined exclusively to the OP. In this work, the genetic control underlying tissue-specific anthocyanin pigmentation in the carrot root OP and IP tissues was investigated by means of linkage mapping and transcriptome (RNA-seq) and phylogenetic analyses; followed by gene expression (RT-qPCR) evaluations in two genetic backgrounds, an F2 population (3242) and the inbred B7262. Genetic mapping of ‘root outer phloem anthocyanin pigmentation’ (ROPAP) and inner phloem pigmentation (RIPAP) revealed colocalization of ROPAP with the P1 and P3 genomic regions previously known to condition pigmentation in different genetic stocks, whereas RIPAP co-localized with P3 only. Transcriptome analysis of purple OP (POP) vs. non-purple IP (NPIP) tissues, along with linkage and phylogenetic data, allowed an initial identification of 28 candidate genes, 19 of which were further evaluated by RT-qPCR in independent root samples of 3242 and B7262, revealing 15 genes consistently upregulated in the POP in both genetic backgrounds, and two genes upregulated in the POP in specific backgrounds. These include seven transcription factors, seven anthocyanin structural genes, and two genes involved in cellular transport. Altogether, our results point at DcMYB7, DcMYB113, and a MADS-box (DCAR_010757) as the main candidate genes conditioning ROPAP in 3242, whereas DcMYB7 and MADS-box condition RIPAP in this background. In 7262, DcMYB113 conditions ROPAP. |
| format | Artículo |
| id | INTA12033 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA120332022-06-07T11:26:30Z Genetic and Transcription Profile Analysis of Tissue-Specific Anthocyanin Pigmentation in Carrot Root Phloem Bannoud, Florencia Carvajal, Sofía Ellison, Shelby L. Senalik, Douglas A. Gomez Talquenca, Gonzalo Iorizzo, Massimo Simon, Philipp W. Cavagnaro, Pablo Zanahoria Daucus carota Variedades Pigmentación Antocianinas Floema Genética Carrots Varieties Pigmentation Anthocyanins Phloem Genetics In purple carrots, anthocyanin pigmentation can be expressed in the entire root, or it can display tissue specific-patterns. Within the phloem, purple pigmentation can be found in the outer phloem (OP) (also called the cortex) and inner phloem (IP), or it can be confined exclusively to the OP. In this work, the genetic control underlying tissue-specific anthocyanin pigmentation in the carrot root OP and IP tissues was investigated by means of linkage mapping and transcriptome (RNA-seq) and phylogenetic analyses; followed by gene expression (RT-qPCR) evaluations in two genetic backgrounds, an F2 population (3242) and the inbred B7262. Genetic mapping of ‘root outer phloem anthocyanin pigmentation’ (ROPAP) and inner phloem pigmentation (RIPAP) revealed colocalization of ROPAP with the P1 and P3 genomic regions previously known to condition pigmentation in different genetic stocks, whereas RIPAP co-localized with P3 only. Transcriptome analysis of purple OP (POP) vs. non-purple IP (NPIP) tissues, along with linkage and phylogenetic data, allowed an initial identification of 28 candidate genes, 19 of which were further evaluated by RT-qPCR in independent root samples of 3242 and B7262, revealing 15 genes consistently upregulated in the POP in both genetic backgrounds, and two genes upregulated in the POP in specific backgrounds. These include seven transcription factors, seven anthocyanin structural genes, and two genes involved in cellular transport. Altogether, our results point at DcMYB7, DcMYB113, and a MADS-box (DCAR_010757) as the main candidate genes conditioning ROPAP in 3242, whereas DcMYB7 and MADS-box condition RIPAP in this background. In 7262, DcMYB113 conditions ROPAP. EEA Mendoza Fil: Bannoud, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carvajal, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ellison, Shelby. University of Wisconsin. Department of Horticulture; Estados Unidos. Fil: Senalik, Douglas A. United States Department of Agriculture–Agricultural Research Service. Vegetable Crops Research Unit; Estados Unidos Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Iorizzo, Massimo. North Carolina State University. Plants for Human Health Institute; Estados Unidos Fil: Iorizzo, Massimo. North Carolina State University. Department of Horticultural Science; Estados Unidos Fil: Simon, Philipp. University of Wisconsin. Department of Horticulture; Estados Unidos. Fil: Simon, Philipp. United States Department of Agriculture–Agricultural Research Service. Vegetable Crops Research Unit; Estados Unidos Fil: Cavagnaro, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina Fil: Cavagnaro, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cavagnaro, Pablo. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Horticultura; Argentina. 2022-06-07T11:24:00Z 2022-06-07T11:24:00Z 2021-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12033 https://www.mdpi.com/2073-4425/12/10/1464 2073-4425 https://doi.org/10.3390/genes12101464 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Genes 12 (10) : 1464 (2021) |
| spellingShingle | Zanahoria Daucus carota Variedades Pigmentación Antocianinas Floema Genética Carrots Varieties Pigmentation Anthocyanins Phloem Genetics Bannoud, Florencia Carvajal, Sofía Ellison, Shelby L. Senalik, Douglas A. Gomez Talquenca, Gonzalo Iorizzo, Massimo Simon, Philipp W. Cavagnaro, Pablo Genetic and Transcription Profile Analysis of Tissue-Specific Anthocyanin Pigmentation in Carrot Root Phloem |
| title | Genetic and Transcription Profile Analysis of Tissue-Specific Anthocyanin Pigmentation in Carrot Root Phloem |
| title_full | Genetic and Transcription Profile Analysis of Tissue-Specific Anthocyanin Pigmentation in Carrot Root Phloem |
| title_fullStr | Genetic and Transcription Profile Analysis of Tissue-Specific Anthocyanin Pigmentation in Carrot Root Phloem |
| title_full_unstemmed | Genetic and Transcription Profile Analysis of Tissue-Specific Anthocyanin Pigmentation in Carrot Root Phloem |
| title_short | Genetic and Transcription Profile Analysis of Tissue-Specific Anthocyanin Pigmentation in Carrot Root Phloem |
| title_sort | genetic and transcription profile analysis of tissue specific anthocyanin pigmentation in carrot root phloem |
| topic | Zanahoria Daucus carota Variedades Pigmentación Antocianinas Floema Genética Carrots Varieties Pigmentation Anthocyanins Phloem Genetics |
| url | http://hdl.handle.net/20.500.12123/12033 https://www.mdpi.com/2073-4425/12/10/1464 https://doi.org/10.3390/genes12101464 |
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