Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize
Maize (Zea mays L.) is a highly valuable crop in Argentina, frequently contaminated with the mycotoxins produced by Aspergillus flavus. Biocontrol products formulated with atoxigenic (nontoxic) strains of this fungal species are well known as an effective method to reduce this contamination. In the...
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| Format: | Artículo |
| Language: | Inglés |
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American Phytopathological Society
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/12018 https://apsjournals.apsnet.org/doi/10.1094/PHYTO-07-17-0255-R https://doi.org/10.1094/PHYTO-07-17-0255-R |
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| author | Camiletti, Boris Xavier Moral, Juan Asensio, Claudia Mariana Torrico Ramallo, Ada Karina Lucini, Enrique Iván Gimenez, Maria De La Paz Michailides, Themis J. |
| author_browse | Asensio, Claudia Mariana Camiletti, Boris Xavier Gimenez, Maria De La Paz Lucini, Enrique Iván Michailides, Themis J. Moral, Juan Torrico Ramallo, Ada Karina |
| author_facet | Camiletti, Boris Xavier Moral, Juan Asensio, Claudia Mariana Torrico Ramallo, Ada Karina Lucini, Enrique Iván Gimenez, Maria De La Paz Michailides, Themis J. |
| author_sort | Camiletti, Boris Xavier |
| collection | INTA Digital |
| description | Maize (Zea mays L.) is a highly valuable crop in Argentina, frequently contaminated with the mycotoxins produced by Aspergillus flavus. Biocontrol products formulated with atoxigenic (nontoxic) strains of this fungal species are well known as an effective method to reduce this contamination. In the present study, 83 A. flavus isolates from two maize regions of Argentina were characterized and evaluated for their ability to produce or lack of producing mycotoxins in order to select atoxigenic strains to be used as potential biocontrol agents (BCA). All of the isolates were tested for aflatoxin and cyclopiazonic acid (CPA) production in maize kernels and a liquid culture medium. Genetic diversity of the nonaflatoxigenic isolates was evaluated by analysis of vegetative compatibility groups (VCG) and confirmation of deletions in the aflatoxin biosynthesis cluster. Eight atoxigenic isolates were compared for their ability to reduce aflatoxin and CPA contamination in maize kernels in coinoculation tests. The A. flavus population was composed of 32% aflatoxin and CPA producers and 52% CPA producers, and 16% was determined as atoxigenic. All of the aflatoxin producer isolates also produced CPA. Aflatoxin and CPA production was significantly higher in maize kernels than in liquid medium. The 57 nonaflatoxigenic strains formed six VCG, with AM1 and AM5 being the dominant groups, with a frequency of 58 and 35%, respectively. In coinoculation experiments, all of the atoxigenic strains reduced aflatoxin from 54 to 83% and CPA from 60 to 97%. Members of group AM1 showed a greater aflatoxin reduction than members of AM5 (72 versus 66%) but no differences were detected in CPA production. Here, we described for the first time atoxigenic isolates of A. flavus that show promise to be used as BCA in maize crops in Argentina. This innovating biological control approach should be considered, developed further, and used by the maize industry to preserve the quality properties and food safety of maize kernels in Argentina. |
| format | Artículo |
| id | INTA12018 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | American Phytopathological Society |
| publisherStr | American Phytopathological Society |
| record_format | dspace |
| spelling | INTA120182022-06-06T10:28:21Z Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize Camiletti, Boris Xavier Moral, Juan Asensio, Claudia Mariana Torrico Ramallo, Ada Karina Lucini, Enrique Iván Gimenez, Maria De La Paz Michailides, Themis J. Aspergillus Flavus Zea Mays Argentina Aflatoxinas Maíz Micotoxinas Maize Mycotoxins Maize (Zea mays L.) is a highly valuable crop in Argentina, frequently contaminated with the mycotoxins produced by Aspergillus flavus. Biocontrol products formulated with atoxigenic (nontoxic) strains of this fungal species are well known as an effective method to reduce this contamination. In the present study, 83 A. flavus isolates from two maize regions of Argentina were characterized and evaluated for their ability to produce or lack of producing mycotoxins in order to select atoxigenic strains to be used as potential biocontrol agents (BCA). All of the isolates were tested for aflatoxin and cyclopiazonic acid (CPA) production in maize kernels and a liquid culture medium. Genetic diversity of the nonaflatoxigenic isolates was evaluated by analysis of vegetative compatibility groups (VCG) and confirmation of deletions in the aflatoxin biosynthesis cluster. Eight atoxigenic isolates were compared for their ability to reduce aflatoxin and CPA contamination in maize kernels in coinoculation tests. The A. flavus population was composed of 32% aflatoxin and CPA producers and 52% CPA producers, and 16% was determined as atoxigenic. All of the aflatoxin producer isolates also produced CPA. Aflatoxin and CPA production was significantly higher in maize kernels than in liquid medium. The 57 nonaflatoxigenic strains formed six VCG, with AM1 and AM5 being the dominant groups, with a frequency of 58 and 35%, respectively. In coinoculation experiments, all of the atoxigenic strains reduced aflatoxin from 54 to 83% and CPA from 60 to 97%. Members of group AM1 showed a greater aflatoxin reduction than members of AM5 (72 versus 66%) but no differences were detected in CPA production. Here, we described for the first time atoxigenic isolates of A. flavus that show promise to be used as BCA in maize crops in Argentina. This innovating biological control approach should be considered, developed further, and used by the maize industry to preserve the quality properties and food safety of maize kernels in Argentina. Instituto de Patología Vegetal Fil: Camiletti, B.X. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Fil: Camiletti, B.X. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina Fil: Moral, Juan. University of Córdoba. Campus de Rabanales. Departamento de Agronomía; España Fil: Moral, Juan. University of California. Kearney Agricultural Research and Extension Center; Estados Unidos Fil: Asensio, Claudia M. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Química Biológica; Argentina Fil: Asensio, Claudia M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Torrico Ramallo, Ada Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Lucini, Enrique I. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Microbiología Agrícola; Argentina Fil: Gimenez, Maria De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Michailides, Themis J. University of California. Kearney Agricultural Research and Extension Center; Estados Unidos 2022-06-06T10:19:51Z 2022-06-06T10:19:51Z 2018-05-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12018 https://apsjournals.apsnet.org/doi/10.1094/PHYTO-07-17-0255-R 0031-949X https://doi.org/10.1094/PHYTO-07-17-0255-R eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Phytopathological Society Phytopathology 108 (7) : 818-828 (2018) |
| spellingShingle | Aspergillus Flavus Zea Mays Argentina Aflatoxinas Maíz Micotoxinas Maize Mycotoxins Camiletti, Boris Xavier Moral, Juan Asensio, Claudia Mariana Torrico Ramallo, Ada Karina Lucini, Enrique Iván Gimenez, Maria De La Paz Michailides, Themis J. Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize |
| title | Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize |
| title_full | Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize |
| title_fullStr | Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize |
| title_full_unstemmed | Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize |
| title_short | Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize |
| title_sort | characterization of argentinian endemic aspergillus flavus isolates and their potential use as biocontrol agents for mycotoxins in maize |
| topic | Aspergillus Flavus Zea Mays Argentina Aflatoxinas Maíz Micotoxinas Maize Mycotoxins |
| url | http://hdl.handle.net/20.500.12123/12018 https://apsjournals.apsnet.org/doi/10.1094/PHYTO-07-17-0255-R https://doi.org/10.1094/PHYTO-07-17-0255-R |
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