The Role of Diversity in Mediating Microbiota Structural and Functional Differences in Two Sympatric Species of Abalone Under Stressed Withering Syndrome Conditions
Withering syndrome (WS) is a gastro-intestinal (GI) infectious disease likely affecting all abalone species worldwide. Structural and functional changes in abalone GI microbiotas under WS-stressed conditions remain poorly investigated. It is unclear if interspecific microbiota differences, such as t...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/12004 https://link.springer.com/article/10.1007/s00248-022-01970-5 https://doi.org/10.1007/s00248-022-01970-5 |
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| author | Cicala, Francesco Cisterna-Céliz, José Alejandro Paolinelli, Marcos Moore, James D. Sevigny, Joseph Rocha-Olivares, Axayácatl |
| author_browse | Cicala, Francesco Cisterna-Céliz, José Alejandro Moore, James D. Paolinelli, Marcos Rocha-Olivares, Axayácatl Sevigny, Joseph |
| author_facet | Cicala, Francesco Cisterna-Céliz, José Alejandro Paolinelli, Marcos Moore, James D. Sevigny, Joseph Rocha-Olivares, Axayácatl |
| author_sort | Cicala, Francesco |
| collection | INTA Digital |
| description | Withering syndrome (WS) is a gastro-intestinal (GI) infectious disease likely affecting all abalone species worldwide. Structural and functional changes in abalone GI microbiotas under WS-stressed conditions remain poorly investigated. It is unclear if interspecific microbiota differences, such as the presence of certain microbes, their abundance, and functional capabilities, may be involved in the occurrence of this disease. Bacterial microbiotas of healthy Haliotis fulgens and Haliotis corrugata are mainly composed by Tenericutes, Proteobacteria, Fusobacteria, and Spirochaetes. We previously reported species-specific structural and functional profiles of those communities and suggested that they are of consequence to the different susceptibility of each species to WS. Here, we address this question by comparing the structure and function of healthy and dysbiotic microbiota through 454 pyrosequencing and PICRUSt 2, respectively. Our findings suggest that the extent to which WS-stressed conditions may explain structural and functional differences in GI microbiota is contingent on the microbiota diversity itself. Indeed, microbiota differences between stressed and healthy abalone were marginal in the more complex bacterial communities of H. corrugata, in which no significant structural or functional changes were detected. Conversely, significant structural changes were observed in the less complex bacterial microbiota of H. fulgens. Moreover, structural alterations led to a significant downregulation of some metabolic activities conducted by GI bacteria. Accordingly, results suggest that gastro-intestinal bacterial diversity appears to be related with both the health of abalone and the etiology of WS. |
| format | info:ar-repo/semantics/artículo |
| id | INTA12004 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA120042022-06-02T13:16:33Z The Role of Diversity in Mediating Microbiota Structural and Functional Differences in Two Sympatric Species of Abalone Under Stressed Withering Syndrome Conditions Cicala, Francesco Cisterna-Céliz, José Alejandro Paolinelli, Marcos Moore, James D. Sevigny, Joseph Rocha-Olivares, Axayácatl Flora Microbiana Oreja de Mar Microbial Flora Abalones Haliotis corrugata Haliotis fulgens Microbiota Síndrome de Marchitamiento Withering Syndrome Withering syndrome (WS) is a gastro-intestinal (GI) infectious disease likely affecting all abalone species worldwide. Structural and functional changes in abalone GI microbiotas under WS-stressed conditions remain poorly investigated. It is unclear if interspecific microbiota differences, such as the presence of certain microbes, their abundance, and functional capabilities, may be involved in the occurrence of this disease. Bacterial microbiotas of healthy Haliotis fulgens and Haliotis corrugata are mainly composed by Tenericutes, Proteobacteria, Fusobacteria, and Spirochaetes. We previously reported species-specific structural and functional profiles of those communities and suggested that they are of consequence to the different susceptibility of each species to WS. Here, we address this question by comparing the structure and function of healthy and dysbiotic microbiota through 454 pyrosequencing and PICRUSt 2, respectively. Our findings suggest that the extent to which WS-stressed conditions may explain structural and functional differences in GI microbiota is contingent on the microbiota diversity itself. Indeed, microbiota differences between stressed and healthy abalone were marginal in the more complex bacterial communities of H. corrugata, in which no significant structural or functional changes were detected. Conversely, significant structural changes were observed in the less complex bacterial microbiota of H. fulgens. Moreover, structural alterations led to a significant downregulation of some metabolic activities conducted by GI bacteria. Accordingly, results suggest that gastro-intestinal bacterial diversity appears to be related with both the health of abalone and the etiology of WS. EEA Mendoza Fil: Cicala, Francesco. CICESE. Department of Biological Oceanography; México Fil: Cicala, Francesco. CICESE. Department of Biomedical Innovation; México Fil: Cisterna-Céliz, José Alejandro. CICESE. Department of Biological Oceanography; México Fil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Paolinelli, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moore, James D. University of California at Davis. Bodega Marine Laboratory; Estados Unidos Fil: Sevigny, Joseph. University of New Hampshire. Hubbard Center for Genome Studies; Estados Unidos Fil: Rocha-Olivares, Axayácatl. CICESE. Department of Biological Oceanography; México 2022-06-02T12:52:45Z 2022-06-02T12:52:45Z 2022-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12004 https://link.springer.com/article/10.1007/s00248-022-01970-5 0095-3628 1432-184X https://doi.org/10.1007/s00248-022-01970-5 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Microbial Ecology (Published: 22 January 2022) |
| spellingShingle | Flora Microbiana Oreja de Mar Microbial Flora Abalones Haliotis corrugata Haliotis fulgens Microbiota Síndrome de Marchitamiento Withering Syndrome Cicala, Francesco Cisterna-Céliz, José Alejandro Paolinelli, Marcos Moore, James D. Sevigny, Joseph Rocha-Olivares, Axayácatl The Role of Diversity in Mediating Microbiota Structural and Functional Differences in Two Sympatric Species of Abalone Under Stressed Withering Syndrome Conditions |
| title | The Role of Diversity in Mediating Microbiota Structural and Functional Differences in Two Sympatric Species of Abalone Under Stressed Withering Syndrome Conditions |
| title_full | The Role of Diversity in Mediating Microbiota Structural and Functional Differences in Two Sympatric Species of Abalone Under Stressed Withering Syndrome Conditions |
| title_fullStr | The Role of Diversity in Mediating Microbiota Structural and Functional Differences in Two Sympatric Species of Abalone Under Stressed Withering Syndrome Conditions |
| title_full_unstemmed | The Role of Diversity in Mediating Microbiota Structural and Functional Differences in Two Sympatric Species of Abalone Under Stressed Withering Syndrome Conditions |
| title_short | The Role of Diversity in Mediating Microbiota Structural and Functional Differences in Two Sympatric Species of Abalone Under Stressed Withering Syndrome Conditions |
| title_sort | role of diversity in mediating microbiota structural and functional differences in two sympatric species of abalone under stressed withering syndrome conditions |
| topic | Flora Microbiana Oreja de Mar Microbial Flora Abalones Haliotis corrugata Haliotis fulgens Microbiota Síndrome de Marchitamiento Withering Syndrome |
| url | http://hdl.handle.net/20.500.12123/12004 https://link.springer.com/article/10.1007/s00248-022-01970-5 https://doi.org/10.1007/s00248-022-01970-5 |
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