Immunomodulatory effect of baculovirus in chickens : how it modifies the immune response against infectious bursal disease virus
Several reports have shown that baculoviruses (BVs) have strong adjuvant properties on the mammalian immune system. Recent studies of our group demonstrated the ability of BV to stimulate the innate immunity in chickens. In this investigation, we aimed to assess the potential antiviral effect of BV...
| Main Authors: | , , , , , , |
|---|---|
| Format: | info:eu-repo/semantics/article |
| Language: | Inglés |
| Published: |
2017
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/1196 https://doi.org/10.1016/j.antiviral.2016.04.004 |
| _version_ | 1855034763956453376 |
|---|---|
| author | Chimeno Zoth, Silvina Andrea Carballeda, Juan Manuel Gravisaco, María José Lucero, Maria Soledad Richetta, Matias Daniel Gomez, Evangelina Raquel Berinstein, Analia |
| author_browse | Berinstein, Analia Carballeda, Juan Manuel Chimeno Zoth, Silvina Andrea Gomez, Evangelina Raquel Gravisaco, María José Lucero, Maria Soledad Richetta, Matias Daniel |
| author_facet | Chimeno Zoth, Silvina Andrea Carballeda, Juan Manuel Gravisaco, María José Lucero, Maria Soledad Richetta, Matias Daniel Gomez, Evangelina Raquel Berinstein, Analia |
| author_sort | Chimeno Zoth, Silvina Andrea |
| collection | INTA Digital |
| description | Several reports have shown that baculoviruses (BVs) have strong adjuvant properties on the mammalian immune system. Recent studies of our group demonstrated the ability of BV to stimulate the innate immunity in chickens. In this investigation, we aimed to assess the potential antiviral effect of BV given both, before and after infectious bursal disease virus (IBDV). In the first case, specific pathogen free chickens were intravenously inoculated with 5 × 10(7) pfu of Autographa californica nuclear polyhedrosis virus and 3 h later were orally administered 2.5 × 10(5) egg infectious doses 50 of IBDV. In the second case, chickens received IBDV 3 h before BV inoculation. Five days later, chickens were bled and euthanized. RNA from the bursa was analyzed for cytokine production. Also, bursae were used for virus recovery, and processed for lymphocyte isolation. The results showed that the administration of BV 3 h after the inoculation with IBDV produced important changes in the effect that IBDV causes in the bursa. BV reduced the infiltration of T lymphocytes, decreased the expression pattern of IL-6 and IFN-γ and inhibited IBDV replication. The results herein presented demonstrate that this Lepidopteran virus shows antiviral activity in chickens under experimental conditions. Investigations under field conditions have to be done to probe this strategy as a valuable sanitary tool for the treatment and prevention of chicken diseases |
| format | info:eu-repo/semantics/article |
| id | INTA1196 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA11962017-11-28T12:58:42Z Immunomodulatory effect of baculovirus in chickens : how it modifies the immune response against infectious bursal disease virus Chimeno Zoth, Silvina Andrea Carballeda, Juan Manuel Gravisaco, María José Lucero, Maria Soledad Richetta, Matias Daniel Gomez, Evangelina Raquel Berinstein, Analia Enfermedades de los Animales Pollo Virus Bursitis Infecciosa Baculovirus Animal Diseases Chickens Infectious Bursal Disease Virus Several reports have shown that baculoviruses (BVs) have strong adjuvant properties on the mammalian immune system. Recent studies of our group demonstrated the ability of BV to stimulate the innate immunity in chickens. In this investigation, we aimed to assess the potential antiviral effect of BV given both, before and after infectious bursal disease virus (IBDV). In the first case, specific pathogen free chickens were intravenously inoculated with 5 × 10(7) pfu of Autographa californica nuclear polyhedrosis virus and 3 h later were orally administered 2.5 × 10(5) egg infectious doses 50 of IBDV. In the second case, chickens received IBDV 3 h before BV inoculation. Five days later, chickens were bled and euthanized. RNA from the bursa was analyzed for cytokine production. Also, bursae were used for virus recovery, and processed for lymphocyte isolation. The results showed that the administration of BV 3 h after the inoculation with IBDV produced important changes in the effect that IBDV causes in the bursa. BV reduced the infiltration of T lymphocytes, decreased the expression pattern of IL-6 and IFN-γ and inhibited IBDV replication. The results herein presented demonstrate that this Lepidopteran virus shows antiviral activity in chickens under experimental conditions. Investigations under field conditions have to be done to probe this strategy as a valuable sanitary tool for the treatment and prevention of chicken diseases Inst. de Biotecnología Fil: Chimeno Zoth, Silvina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carballeda, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Lucero, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Richetta, Matias Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Agencia Nacional de Promoción Científica y Técnica; Argentina Fil: Gomez, Evangelina Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Berinstein, Analia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-09-11T16:42:29Z 2017-09-11T16:42:29Z 2016 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1196 0166-3542 (Print) 1872-9096 (Online) https://doi.org/10.1016/j.antiviral.2016.04.004 eng info:eu-repo/semantics/restrictedAccess application/pdf Antiviral Research 131 : 35-39 (July 2016) |
| spellingShingle | Enfermedades de los Animales Pollo Virus Bursitis Infecciosa Baculovirus Animal Diseases Chickens Infectious Bursal Disease Virus Chimeno Zoth, Silvina Andrea Carballeda, Juan Manuel Gravisaco, María José Lucero, Maria Soledad Richetta, Matias Daniel Gomez, Evangelina Raquel Berinstein, Analia Immunomodulatory effect of baculovirus in chickens : how it modifies the immune response against infectious bursal disease virus |
| title | Immunomodulatory effect of baculovirus in chickens : how it modifies the immune response against infectious bursal disease virus |
| title_full | Immunomodulatory effect of baculovirus in chickens : how it modifies the immune response against infectious bursal disease virus |
| title_fullStr | Immunomodulatory effect of baculovirus in chickens : how it modifies the immune response against infectious bursal disease virus |
| title_full_unstemmed | Immunomodulatory effect of baculovirus in chickens : how it modifies the immune response against infectious bursal disease virus |
| title_short | Immunomodulatory effect of baculovirus in chickens : how it modifies the immune response against infectious bursal disease virus |
| title_sort | immunomodulatory effect of baculovirus in chickens how it modifies the immune response against infectious bursal disease virus |
| topic | Enfermedades de los Animales Pollo Virus Bursitis Infecciosa Baculovirus Animal Diseases Chickens Infectious Bursal Disease Virus |
| url | http://hdl.handle.net/20.500.12123/1196 https://doi.org/10.1016/j.antiviral.2016.04.004 |
| work_keys_str_mv | AT chimenozothsilvinaandrea immunomodulatoryeffectofbaculovirusinchickenshowitmodifiestheimmuneresponseagainstinfectiousbursaldiseasevirus AT carballedajuanmanuel immunomodulatoryeffectofbaculovirusinchickenshowitmodifiestheimmuneresponseagainstinfectiousbursaldiseasevirus AT gravisacomariajose immunomodulatoryeffectofbaculovirusinchickenshowitmodifiestheimmuneresponseagainstinfectiousbursaldiseasevirus AT luceromariasoledad immunomodulatoryeffectofbaculovirusinchickenshowitmodifiestheimmuneresponseagainstinfectiousbursaldiseasevirus AT richettamatiasdaniel immunomodulatoryeffectofbaculovirusinchickenshowitmodifiestheimmuneresponseagainstinfectiousbursaldiseasevirus AT gomezevangelinaraquel immunomodulatoryeffectofbaculovirusinchickenshowitmodifiestheimmuneresponseagainstinfectiousbursaldiseasevirus AT berinsteinanalia immunomodulatoryeffectofbaculovirusinchickenshowitmodifiestheimmuneresponseagainstinfectiousbursaldiseasevirus |