Phenotypic Variability and Genetic Diversity of the Pathogenic Fungus Macrophomina phaseolina from Several Hosts and Host Specialization in Strawberry
Macrophomina phaseolina, is a pathogenic soil-borne fungus that affects more than 500 plant species, causing various types of disease to several crops, among which is the crown and root rot disease in strawberry. Its wide variability has been characterized reiteratively in the literature, but little...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2022
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/11943 https://link.springer.com/article/10.1007/s00284-022-02883-9 https://doi.org/10.1007/s00284-022-02883-9 |
| _version_ | 1855484878469988352 |
|---|---|
| author | Viejobueno, Josefina Santos, Berta de los Camacho-Sánchez, Miguel Aguado, Ana Camacho, María Salazar, Sergio Miguel |
| author_browse | Aguado, Ana Camacho, María Camacho-Sánchez, Miguel Salazar, Sergio Miguel Santos, Berta de los Viejobueno, Josefina |
| author_facet | Viejobueno, Josefina Santos, Berta de los Camacho-Sánchez, Miguel Aguado, Ana Camacho, María Salazar, Sergio Miguel |
| author_sort | Viejobueno, Josefina |
| collection | INTA Digital |
| description | Macrophomina phaseolina, is a pathogenic soil-borne fungus that affects more than 500 plant species, causing various types of disease to several crops, among which is the crown and root rot disease in strawberry. Its wide variability has been characterized reiteratively in the literature, but little is known about its virulence mechanisms. Morphological, physiological, genetic and phytopathogenic parameters were evaluated among 32 isolates of Macrophomina from different hosts occurring in Argentina and Spain. Colony characteristics, average size of microsclerotia, chlorate phenotype and mycelial growth at different temperatures (5º–40 °C), and pH (3.0–8.0) were recorded. The morphological and physiological traits were heterogeneous and did not show any association with the genetic structure nor with their pathogenicity. Most of the isolates (71.9%) exhibited chlorate-sensitive phenotype. The optimal growth temperature range was between 25 °C and 35 °C, and the optimal pH varied between 4.0 and 6.0. The genetic structure analyzed with four DNA markers (EF-1α, ITS, CAL and TUB) showed little diversity among isolates of M. phaseolina, with no clear association with the country of origin, but a significant association with the host. Based on their phylogenetic affinity, one isolate was reclassified as M. pseudophaseolina and another one as M. tecta. It is the first report of M. pseudophaseolina causing charcoal rot on beans, in Argentina, and the first report of M. tecta outside Australia. Pathogenicity tests on strawberry plants revealed marked host specialization, being the isolates obtained from strawberry more virulent than those from other hosts. |
| format | Artículo |
| id | INTA11943 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA119432022-05-24T12:40:03Z Phenotypic Variability and Genetic Diversity of the Pathogenic Fungus Macrophomina phaseolina from Several Hosts and Host Specialization in Strawberry Viejobueno, Josefina Santos, Berta de los Camacho-Sánchez, Miguel Aguado, Ana Camacho, María Salazar, Sergio Miguel Fresa Variación Fenotípica Variación Genética Macrophomina phaseolina Enfermedades Fungosas Strawberries Phenotypic Variation Genetic Variation Fungal Diseases Frutilla Macrophomina phaseolina, is a pathogenic soil-borne fungus that affects more than 500 plant species, causing various types of disease to several crops, among which is the crown and root rot disease in strawberry. Its wide variability has been characterized reiteratively in the literature, but little is known about its virulence mechanisms. Morphological, physiological, genetic and phytopathogenic parameters were evaluated among 32 isolates of Macrophomina from different hosts occurring in Argentina and Spain. Colony characteristics, average size of microsclerotia, chlorate phenotype and mycelial growth at different temperatures (5º–40 °C), and pH (3.0–8.0) were recorded. The morphological and physiological traits were heterogeneous and did not show any association with the genetic structure nor with their pathogenicity. Most of the isolates (71.9%) exhibited chlorate-sensitive phenotype. The optimal growth temperature range was between 25 °C and 35 °C, and the optimal pH varied between 4.0 and 6.0. The genetic structure analyzed with four DNA markers (EF-1α, ITS, CAL and TUB) showed little diversity among isolates of M. phaseolina, with no clear association with the country of origin, but a significant association with the host. Based on their phylogenetic affinity, one isolate was reclassified as M. pseudophaseolina and another one as M. tecta. It is the first report of M. pseudophaseolina causing charcoal rot on beans, in Argentina, and the first report of M. tecta outside Australia. Pathogenicity tests on strawberry plants revealed marked host specialization, being the isolates obtained from strawberry more virulent than those from other hosts. EEA Famaillá Fil: Viejobueno, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Santos, Berta de los. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica; España Fil: Camacho-Sánchez, Miguel. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica; España Fil: Aguado, Ana. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica; España Fil: Camacho, María. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica; España Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Salazar, Sergio Miguel. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina 2022-05-24T12:37:55Z 2022-05-24T12:37:55Z 2022-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11943 https://link.springer.com/article/10.1007/s00284-022-02883-9 0343-8651 1432-0991 https://doi.org/10.1007/s00284-022-02883-9 eng info:eu-repograntAgreement/INTA/PNHFA-1106073/AR./Aumento de la competitividad con sustentabilidad y equidad social de sistemas productivos de hortalizas frescas diferenciadas. info:eu-repograntAgreement/INTA/2019-PD-E4-I069-001/2019-PD-E4-I069-001/AR./Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetal info:eu-repograntAgreement/INTA/2019-PD-E4-I085-001/2019-PD-E4-I085-001/AR./Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno. info:eu-repo/semantics/restrictedAccess application/pdf Springer Current Microbiology 79 : article number: 189 (2022) |
| spellingShingle | Fresa Variación Fenotípica Variación Genética Macrophomina phaseolina Enfermedades Fungosas Strawberries Phenotypic Variation Genetic Variation Fungal Diseases Frutilla Viejobueno, Josefina Santos, Berta de los Camacho-Sánchez, Miguel Aguado, Ana Camacho, María Salazar, Sergio Miguel Phenotypic Variability and Genetic Diversity of the Pathogenic Fungus Macrophomina phaseolina from Several Hosts and Host Specialization in Strawberry |
| title | Phenotypic Variability and Genetic Diversity of the Pathogenic Fungus Macrophomina phaseolina from Several Hosts and Host Specialization in Strawberry |
| title_full | Phenotypic Variability and Genetic Diversity of the Pathogenic Fungus Macrophomina phaseolina from Several Hosts and Host Specialization in Strawberry |
| title_fullStr | Phenotypic Variability and Genetic Diversity of the Pathogenic Fungus Macrophomina phaseolina from Several Hosts and Host Specialization in Strawberry |
| title_full_unstemmed | Phenotypic Variability and Genetic Diversity of the Pathogenic Fungus Macrophomina phaseolina from Several Hosts and Host Specialization in Strawberry |
| title_short | Phenotypic Variability and Genetic Diversity of the Pathogenic Fungus Macrophomina phaseolina from Several Hosts and Host Specialization in Strawberry |
| title_sort | phenotypic variability and genetic diversity of the pathogenic fungus macrophomina phaseolina from several hosts and host specialization in strawberry |
| topic | Fresa Variación Fenotípica Variación Genética Macrophomina phaseolina Enfermedades Fungosas Strawberries Phenotypic Variation Genetic Variation Fungal Diseases Frutilla |
| url | http://hdl.handle.net/20.500.12123/11943 https://link.springer.com/article/10.1007/s00284-022-02883-9 https://doi.org/10.1007/s00284-022-02883-9 |
| work_keys_str_mv | AT viejobuenojosefina phenotypicvariabilityandgeneticdiversityofthepathogenicfungusmacrophominaphaseolinafromseveralhostsandhostspecializationinstrawberry AT santosbertadelos phenotypicvariabilityandgeneticdiversityofthepathogenicfungusmacrophominaphaseolinafromseveralhostsandhostspecializationinstrawberry AT camachosanchezmiguel phenotypicvariabilityandgeneticdiversityofthepathogenicfungusmacrophominaphaseolinafromseveralhostsandhostspecializationinstrawberry AT aguadoana phenotypicvariabilityandgeneticdiversityofthepathogenicfungusmacrophominaphaseolinafromseveralhostsandhostspecializationinstrawberry AT camachomaria phenotypicvariabilityandgeneticdiversityofthepathogenicfungusmacrophominaphaseolinafromseveralhostsandhostspecializationinstrawberry AT salazarsergiomiguel phenotypicvariabilityandgeneticdiversityofthepathogenicfungusmacrophominaphaseolinafromseveralhostsandhostspecializationinstrawberry |