Isolation and clinical sample typing of human leptospirosis cases in Argentina
Leptospira typing is carried out using isolated strains. Because of difficulties in obtaining them, direct identification of infective Leptospira in clinical samples is a high priority.Multilocus sequence typing (MLST) proved highly discriminatory for seven pathogenic species of Leptospira, allowing...
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| Format: | Artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1192 http://www.sciencedirect.com/science/article/pii/S1567134815300630 https://doi.org/10.1016/j.meegid.2015.11.033 |
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| author | Varni, Vanina Delia Caimi, Karina Cynthia Jacob, Paulina Chiani, Yosena Landolt, Noelia Schmeling, María Fernanda Pujato, Nazarena Vanasco, Bibiana |
| author_browse | Caimi, Karina Cynthia Chiani, Yosena Jacob, Paulina Landolt, Noelia Pujato, Nazarena Schmeling, María Fernanda Vanasco, Bibiana Varni, Vanina Delia |
| author_facet | Varni, Vanina Delia Caimi, Karina Cynthia Jacob, Paulina Chiani, Yosena Landolt, Noelia Schmeling, María Fernanda Pujato, Nazarena Vanasco, Bibiana |
| author_sort | Varni, Vanina Delia |
| collection | INTA Digital |
| description | Leptospira typing is carried out using isolated strains. Because of difficulties in obtaining them, direct identification of infective Leptospira in clinical samples is a high priority.Multilocus sequence typing (MLST) proved highly discriminatory for seven pathogenic species of Leptospira, allowing isolate characterization and robust assignment to species, in addition to phylogenetic evidence for the relatedness between species. In this study we characterized Leptospira strains circulating in Argentina, using typing methods applied to human clinical samples and isolates. Phylogenetic studies based on 16S ribosomal RNA gene sequences enabled typing of 8 isolates (6 Leptospira interrogans, one Leptospirawolffii and one Leptospira broomii) and 58 out of 85 (68.2%) clinical samples (55 L. interrogans, 2 Leptospira meyeri, and one Leptospira kirschneri). MLST results for the L. interrogans isolates indicated that five were probably Canicola serogroup (ST37) and one was probably Icterohaemorrhagiae serogroup (ST17). Eleven clinical samples (21.6%), provided MLST interpretable data: five were probably Pyrogenes serogroup (ST13), four Sejroe (ST20), one Autumnalis (ST22) and one Canicola (ST37). To the best of our knowledge this study is the first report of the use of an MLST typing scheme with seven loci to identify
Leptospira directly from clinical samples in Argentina. The use of clinical samples presents the advantage of the possibility of knowing the infecting strain without resorting to isolates. This study also allowed, for the first time, the characterization of isolates of intermediate pathogenicity species (L. wolffii and L. broomii) from symptomatic patients. |
| format | Artículo |
| id | INTA1192 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA11922019-03-22T13:46:34Z Isolation and clinical sample typing of human leptospirosis cases in Argentina Varni, Vanina Delia Caimi, Karina Cynthia Jacob, Paulina Chiani, Yosena Landolt, Noelia Schmeling, María Fernanda Pujato, Nazarena Vanasco, Bibiana Leptospirosis Enfermedades Humanas Ensayos Clínicos Técnicas de Aislamiento Human Diseases Clinical Trials Isolation Techniques Leptospira typing is carried out using isolated strains. Because of difficulties in obtaining them, direct identification of infective Leptospira in clinical samples is a high priority.Multilocus sequence typing (MLST) proved highly discriminatory for seven pathogenic species of Leptospira, allowing isolate characterization and robust assignment to species, in addition to phylogenetic evidence for the relatedness between species. In this study we characterized Leptospira strains circulating in Argentina, using typing methods applied to human clinical samples and isolates. Phylogenetic studies based on 16S ribosomal RNA gene sequences enabled typing of 8 isolates (6 Leptospira interrogans, one Leptospirawolffii and one Leptospira broomii) and 58 out of 85 (68.2%) clinical samples (55 L. interrogans, 2 Leptospira meyeri, and one Leptospira kirschneri). MLST results for the L. interrogans isolates indicated that five were probably Canicola serogroup (ST37) and one was probably Icterohaemorrhagiae serogroup (ST17). Eleven clinical samples (21.6%), provided MLST interpretable data: five were probably Pyrogenes serogroup (ST13), four Sejroe (ST20), one Autumnalis (ST22) and one Canicola (ST37). To the best of our knowledge this study is the first report of the use of an MLST typing scheme with seven loci to identify Leptospira directly from clinical samples in Argentina. The use of clinical samples presents the advantage of the possibility of knowing the infecting strain without resorting to isolates. This study also allowed, for the first time, the characterization of isolates of intermediate pathogenicity species (L. wolffii and L. broomii) from symptomatic patients. Inst. de Biotecnología Fil: Varni, Vanina Delia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Jacob, Paulina. Instituto Nacional de Enfermedades Respiratorias “Dr. E. Coni”; Argentina. Administración Nacional de Laboratorios e Institutos de Salud; Argentina Fil: Landolt, Noelia. Instituto Nacional de Enfermedades Respiratorias “Dr. E. Coni”; Argentina. Administración Nacional de Laboratorios e Institutos de Salud; Argentina Fil: Schmeling, María Fernanda. Instituto Nacional de Enfermedades Respiratorias “Dr. E. Coni”; Argentina. Administración Nacional de Laboratorios e Institutos de Salud; Argentina Fil: Pujato, Nazarena. Instituto Nacional de Enfermedades Respiratorias “Dr. E. Coni”; Argentina. Administración Nacional de Laboratorios e Institutos de Salud; Argentina Fil: Vanasco, Bibiana. Instituto Nacional de Enfermedades Respiratorias “Dr. E. Coni”; Argentina. Administración Nacional de Laboratorios e Institutos de Salud; Argentina 2017-09-11T14:46:23Z 2017-09-11T14:46:23Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1192 http://www.sciencedirect.com/science/article/pii/S1567134815300630 1567-1348 (Print) 1567-7257 (Online) https://doi.org/10.1016/j.meegid.2015.11.033 eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) Infection, genetics and evolution 37 : 245-251. (January 2016) |
| spellingShingle | Leptospirosis Enfermedades Humanas Ensayos Clínicos Técnicas de Aislamiento Human Diseases Clinical Trials Isolation Techniques Varni, Vanina Delia Caimi, Karina Cynthia Jacob, Paulina Chiani, Yosena Landolt, Noelia Schmeling, María Fernanda Pujato, Nazarena Vanasco, Bibiana Isolation and clinical sample typing of human leptospirosis cases in Argentina |
| title | Isolation and clinical sample typing of human leptospirosis cases in Argentina |
| title_full | Isolation and clinical sample typing of human leptospirosis cases in Argentina |
| title_fullStr | Isolation and clinical sample typing of human leptospirosis cases in Argentina |
| title_full_unstemmed | Isolation and clinical sample typing of human leptospirosis cases in Argentina |
| title_short | Isolation and clinical sample typing of human leptospirosis cases in Argentina |
| title_sort | isolation and clinical sample typing of human leptospirosis cases in argentina |
| topic | Leptospirosis Enfermedades Humanas Ensayos Clínicos Técnicas de Aislamiento Human Diseases Clinical Trials Isolation Techniques |
| url | http://hdl.handle.net/20.500.12123/1192 http://www.sciencedirect.com/science/article/pii/S1567134815300630 https://doi.org/10.1016/j.meegid.2015.11.033 |
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