Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv a...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1187 https://academic.oup.com/jxb/article/67/14/4091/2197644 https://doi.org/10.1093/jxb/erw185 |
| _version_ | 1855482844027027456 |
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| author | Coluccio Leskow, Carla Dominguez, Pia Guadalupe Díaz Zirpolo, José Antonio Obata, Toshihiro Kamenetzky, Laura Costa, Hernán Taboga, Oscar Alberto Marti, Hector Ruben Keurentjes, Joost Sulpice, Ronan Ishihara, Hirofumi Stitt, Mark Fernie, Alisdair R. Carrari, Fernando |
| author_browse | Carrari, Fernando Coluccio Leskow, Carla Costa, Hernán Dominguez, Pia Guadalupe Díaz Zirpolo, José Antonio Fernie, Alisdair R. Ishihara, Hirofumi Kamenetzky, Laura Keurentjes, Joost Marti, Hector Ruben Obata, Toshihiro Stitt, Mark Sulpice, Ronan Taboga, Oscar Alberto |
| author_facet | Coluccio Leskow, Carla Dominguez, Pia Guadalupe Díaz Zirpolo, José Antonio Obata, Toshihiro Kamenetzky, Laura Costa, Hernán Taboga, Oscar Alberto Marti, Hector Ruben Keurentjes, Joost Sulpice, Ronan Ishihara, Hirofumi Stitt, Mark Fernie, Alisdair R. Carrari, Fernando |
| author_sort | Coluccio Leskow, Carla |
| collection | INTA Digital |
| description | Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde Island (CVI), with the CVI allele conferring both higher Inv activity and longer radicles. The aim of the current work is to understand the mechanism(s) underlying this QTL by analyzing structural and functional differences of vac-Inv from both accessions. Relative transcript abundance analyzed by quantitative real-time PCR (qRT-PCR) showed similar expression patterns in both accessions; however, DNA sequence analyses revealed several polymorphisms that lead to changes in the corresponding protein sequence. Moreover, activity assays revealed higher vac-Inv activity in genotypes carrying the CVI allele than in those carrying the Ler allele. Analyses of purified recombinant proteins showed a similar K m for both alleles and a slightly higher V max for that of Ler. Treatment of plant extracts with foaming to release possible interacting Inv inhibitory protein(s) led to a large increase in activity for the Ler allele, but no changes for genotypes carrying the CVI allele. qRT-PCR analyses of two vac-Inv inhibitors in seedlings from parental and NIL genotypes revealed different expression patterns. Taken together, these results demonstrate that the vac-Inv QTL affects root biomass accumulation and also carbon partitioning through a differential regulation of vac-Inv inhibitors at the mRNA level. |
| format | Artículo |
| id | INTA1187 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA11872019-02-22T14:18:03Z Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development Coluccio Leskow, Carla Dominguez, Pia Guadalupe Díaz Zirpolo, José Antonio Obata, Toshihiro Kamenetzky, Laura Costa, Hernán Taboga, Oscar Alberto Marti, Hector Ruben Keurentjes, Joost Sulpice, Ronan Ishihara, Hirofumi Stitt, Mark Fernie, Alisdair R. Carrari, Fernando Fitomejoramiento Arabidopsis Biomasa Loci de Rasgos Cuantitativos Plant Breeding Biomass Quantitative Trait Loci Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde Island (CVI), with the CVI allele conferring both higher Inv activity and longer radicles. The aim of the current work is to understand the mechanism(s) underlying this QTL by analyzing structural and functional differences of vac-Inv from both accessions. Relative transcript abundance analyzed by quantitative real-time PCR (qRT-PCR) showed similar expression patterns in both accessions; however, DNA sequence analyses revealed several polymorphisms that lead to changes in the corresponding protein sequence. Moreover, activity assays revealed higher vac-Inv activity in genotypes carrying the CVI allele than in those carrying the Ler allele. Analyses of purified recombinant proteins showed a similar K m for both alleles and a slightly higher V max for that of Ler. Treatment of plant extracts with foaming to release possible interacting Inv inhibitory protein(s) led to a large increase in activity for the Ler allele, but no changes for genotypes carrying the CVI allele. qRT-PCR analyses of two vac-Inv inhibitors in seedlings from parental and NIL genotypes revealed different expression patterns. Taken together, these results demonstrate that the vac-Inv QTL affects root biomass accumulation and also carbon partitioning through a differential regulation of vac-Inv inhibitors at the mRNA level. Inst. de Biotecnología Fil: Coluccio Leskow, Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Díaz Zirpolo, José Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Obata, Toshihiro. Max Planck Institute for Molecular Plant Physiology; Alemania Fil: Kamenetzky, Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Marti, Marcelo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Keurentjes, Joost. Wageningen University. Laboratory of Genetics; Holanda Fil: Sulpice, Ronan. Max Planck Institute for Molecular Plant Physiology; Alemania Fil: Ishihara, Hirofumi. Max Planck Institute for Molecular Plant Physiology; Alemania Fil: Stitt, Mark. Max Planck Institute for Molecular Plant Physiology; Alemania Fil: Fernie, Alisdair R. Max Planck Institute for Molecular Plant Physiology; Alemania Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2017-09-11T12:55:58Z 2017-09-11T12:55:58Z 2016 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1187 https://academic.oup.com/jxb/article/67/14/4091/2197644 0022-0957 (Print) 1460-2431 (Online) https://doi.org/10.1093/jxb/erw185 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Journal of experimental botany 67 (14) : 4091–4103 (July 2016) |
| spellingShingle | Fitomejoramiento Arabidopsis Biomasa Loci de Rasgos Cuantitativos Plant Breeding Biomass Quantitative Trait Loci Coluccio Leskow, Carla Dominguez, Pia Guadalupe Díaz Zirpolo, José Antonio Obata, Toshihiro Kamenetzky, Laura Costa, Hernán Taboga, Oscar Alberto Marti, Hector Ruben Keurentjes, Joost Sulpice, Ronan Ishihara, Hirofumi Stitt, Mark Fernie, Alisdair R. Carrari, Fernando Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development |
| title | Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development |
| title_full | Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development |
| title_fullStr | Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development |
| title_full_unstemmed | Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development |
| title_short | Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development |
| title_sort | allelic differences in a vacuolar invertase affect arabidopsis growth at early plant development |
| topic | Fitomejoramiento Arabidopsis Biomasa Loci de Rasgos Cuantitativos Plant Breeding Biomass Quantitative Trait Loci |
| url | http://hdl.handle.net/20.500.12123/1187 https://academic.oup.com/jxb/article/67/14/4091/2197644 https://doi.org/10.1093/jxb/erw185 |
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