Polymorphisms of 20 regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis

Mycobacterium tuberculosis and Mycobacterium bovis are responsible for tuberculosis in humans and animals, respectively. Both species are closely related and belong to the Mycobacterium tuberculosis complex (MTC). M. tuberculosis is the most ancient species from which M. bovis and other members of t...

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Autores principales: Bigi, María Mercedes, Blanco, Federico Carlos, Araujo, Flabio Ribeiro de, Thacker, Tyler C., Zumarraga, Martin Jose, Cataldi, Angel Adrian, Soria, Marcelo Abel, Bigi, Fabiana
Formato: Artículo
Lenguaje:Inglés
Publicado: 2017
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/1186
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author Bigi, María Mercedes
Blanco, Federico Carlos
Araujo, Flabio Ribeiro de
Thacker, Tyler C.
Zumarraga, Martin Jose
Cataldi, Angel Adrian
Soria, Marcelo Abel
Bigi, Fabiana
author_browse Araujo, Flabio Ribeiro de
Bigi, Fabiana
Bigi, María Mercedes
Blanco, Federico Carlos
Cataldi, Angel Adrian
Soria, Marcelo Abel
Thacker, Tyler C.
Zumarraga, Martin Jose
author_facet Bigi, María Mercedes
Blanco, Federico Carlos
Araujo, Flabio Ribeiro de
Thacker, Tyler C.
Zumarraga, Martin Jose
Cataldi, Angel Adrian
Soria, Marcelo Abel
Bigi, Fabiana
author_sort Bigi, María Mercedes
collection INTA Digital
description Mycobacterium tuberculosis and Mycobacterium bovis are responsible for tuberculosis in humans and animals, respectively. Both species are closely related and belong to the Mycobacterium tuberculosis complex (MTC). M. tuberculosis is the most ancient species from which M. bovis and other members of the MTC evolved. The genome of M. bovis is over >99.95% identical to that of M. tuberculosis but with seven deletions ranging in size from 1 to 12.7 kb. In addition, 1200 single nucleotide mutations in coding regions distinguish M. bovis from M. tuberculosis. In the present study, we assessed 75 M. tuberculosis genomes and 23 M. bovis genomes to identify non-synonymous mutations in 202 coding sequences of regulatory genes between both species. We identified species-specific variants in 20 regulatory proteins and confirmed differential expression of hypoxia-related genes between M. bovis and M. tuberculosis
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spelling INTA11862019-06-05T16:49:39Z Polymorphisms of 20 regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis Bigi, María Mercedes Blanco, Federico Carlos Araujo, Flabio Ribeiro de Thacker, Tyler C. Zumarraga, Martin Jose Cataldi, Angel Adrian Soria, Marcelo Abel Bigi, Fabiana Enfermedades de los Animales Mycobacterium Tuberculosis Mycobacterium Bovis Enfermedades Infecciosas Polimorfismo Animal Diseases Infectious Diseases Polymorphism Mycobacterium tuberculosis and Mycobacterium bovis are responsible for tuberculosis in humans and animals, respectively. Both species are closely related and belong to the Mycobacterium tuberculosis complex (MTC). M. tuberculosis is the most ancient species from which M. bovis and other members of the MTC evolved. The genome of M. bovis is over >99.95% identical to that of M. tuberculosis but with seven deletions ranging in size from 1 to 12.7 kb. In addition, 1200 single nucleotide mutations in coding regions distinguish M. bovis from M. tuberculosis. In the present study, we assessed 75 M. tuberculosis genomes and 23 M. bovis genomes to identify non-synonymous mutations in 202 coding sequences of regulatory genes between both species. We identified species-specific variants in 20 regulatory proteins and confirmed differential expression of hypoxia-related genes between M. bovis and M. tuberculosis Inst. de Biotecnología Fil: Bigi, María Mercedes. Universidad de Buenos Aires; Argentina Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Araujo, Flabio R. EMBRAPA Mato Grosso do Sul; Brasil Fil: Thacker, Tyler C. United States Department of Agriculture. Agricultural Research Service. National Animal Disease Center; Estados Unidos Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2017-09-11T12:26:47Z 2017-09-11T12:26:47Z 2016 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1186 0385-5600 (Print) 1348-0421 (Online) DOI: 10.1111/1348-0421.12402 eng info:eu-repo/semantics/restrictedAccess application/pdf Microbiology and Immunology 60 (8) : 552–560. (August 2016)
spellingShingle Enfermedades de los Animales
Mycobacterium Tuberculosis
Mycobacterium Bovis
Enfermedades Infecciosas
Polimorfismo
Animal Diseases
Infectious Diseases
Polymorphism
Bigi, María Mercedes
Blanco, Federico Carlos
Araujo, Flabio Ribeiro de
Thacker, Tyler C.
Zumarraga, Martin Jose
Cataldi, Angel Adrian
Soria, Marcelo Abel
Bigi, Fabiana
Polymorphisms of 20 regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis
title Polymorphisms of 20 regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis
title_full Polymorphisms of 20 regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis
title_fullStr Polymorphisms of 20 regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis
title_full_unstemmed Polymorphisms of 20 regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis
title_short Polymorphisms of 20 regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis
title_sort polymorphisms of 20 regulatory proteins between mycobacterium tuberculosis and mycobacterium bovis
topic Enfermedades de los Animales
Mycobacterium Tuberculosis
Mycobacterium Bovis
Enfermedades Infecciosas
Polimorfismo
Animal Diseases
Infectious Diseases
Polymorphism
url http://hdl.handle.net/20.500.12123/1186
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