Development and field evaluation of a nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) analysis to identify A. marginale-infected and A. centrale-vaccinated cattle

A nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) assay based on the amplification of the Anaplasma spp. highly conserved msp5 gene and posterior digestion with HindIII endonuclease was developed and evaluated in field samples. Results were compared using an nPC...

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Bibliographic Details

Main Authors:	Primo, María Evangelina, Bellezze, Julio, Morel, Nicolas, Mazzucco Panizza, Matilde Nahimé, Valentini, Beatriz Susana, Torioni, Susana Marta, Thompson, Carolina Soledad
Format:	info:ar-repo/semantics/artículo
Language:	Inglés
Published:	Elsevier 2022
Subjects:	Ganado Bovino RFLP Anaplasma marginale Anaplasma centrale Experimentación en Campo Cattle PCR Restriction Fragment Length Polymorphism Field Experimentation
Online Access:	http://hdl.handle.net/20.500.12123/11835 https://www.sciencedirect.com/science/article/abs/pii/S1877959X22000577 https://doi.org/10.1016/j.ttbdis.2022.101952