Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry
The effect of antagonistic bacteria to control Macrophomina phaseolina was evaluated under in vitro, growth chamber, greenhouse and field conditions. A total of 177 bacteria, isolated from Athrocaulon macrostachyum rhizosphere of the Lebrija marsh, were screened for their potential against M. phaseo...
| Main Authors: | , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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MDPI
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/11797 https://www.mdpi.com/2311-7524/7/11/457 https://doi.org/10.3390/horticulturae7110457 |
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| author | Viejobueno, Josefina Rodríguez-Berbel, Natalia Miranda, Luis Santos, Berta de los Camacho, María |
| author_browse | Camacho, María Miranda, Luis Rodríguez-Berbel, Natalia Santos, Berta de los Viejobueno, Josefina |
| author_facet | Viejobueno, Josefina Rodríguez-Berbel, Natalia Miranda, Luis Santos, Berta de los Camacho, María |
| author_sort | Viejobueno, Josefina |
| collection | INTA Digital |
| description | The effect of antagonistic bacteria to control Macrophomina phaseolina was evaluated under in vitro, growth chamber, greenhouse and field conditions. A total of 177 bacteria, isolated from Athrocaulon macrostachyum rhizosphere of the Lebrija marsh, were screened for their potential against M. phaseolina (causes charcoal rot in strawberry) by dual culture assay. Of these isolates, 14 most promising strains were molecularly identified by the 16S rDNA sequencing method using the EzBioCloud database. These strains were tested for in vitro hydrolytic enzymes, HCN production, and biocontrol against M. phaseolina in strawberry plants. All the 14 strains produced, at least, one hydrolytic enzymatic activity and one of them, which belongs to Brevibacterium genus (Hvs8), showed the lowest records of disease incidence (20%) and severity (0.4). With these results, greenhouse and field trials were carried out with the Hvs8 strain, compared to non-treated control. In the greenhouse assays, Hvs8 strain increased root dry mass by 30%, over the control. In the field trials, production and fruit quality were not significantly different between Hvs8 treatment and non-treated control, but plant mortality and plant mortality associated to M. phaseolina decreased by more than 24% and 65% respectively, in Hvs8 treatment. This study suggests that Brevibacterium sp. Hvs8 strain could be a candidate for controlling charcoal rot in strawberry. |
| format | Artículo |
| id | INTA11797 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA117972022-05-03T17:15:37Z Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry Viejobueno, Josefina Rodríguez-Berbel, Natalia Miranda, Luis Santos, Berta de los Camacho, María Fresa Enfermedades de las Plantas Control de Enfermedades de Plantas Macrophomina phaseolina Control Biológico Bacterias Antagonistas Strawberries Plant Diseases Plant Disease Control Biological Control Antagonistic Bacteria Frutilla Charcoal Rot Disease The effect of antagonistic bacteria to control Macrophomina phaseolina was evaluated under in vitro, growth chamber, greenhouse and field conditions. A total of 177 bacteria, isolated from Athrocaulon macrostachyum rhizosphere of the Lebrija marsh, were screened for their potential against M. phaseolina (causes charcoal rot in strawberry) by dual culture assay. Of these isolates, 14 most promising strains were molecularly identified by the 16S rDNA sequencing method using the EzBioCloud database. These strains were tested for in vitro hydrolytic enzymes, HCN production, and biocontrol against M. phaseolina in strawberry plants. All the 14 strains produced, at least, one hydrolytic enzymatic activity and one of them, which belongs to Brevibacterium genus (Hvs8), showed the lowest records of disease incidence (20%) and severity (0.4). With these results, greenhouse and field trials were carried out with the Hvs8 strain, compared to non-treated control. In the greenhouse assays, Hvs8 strain increased root dry mass by 30%, over the control. In the field trials, production and fruit quality were not significantly different between Hvs8 treatment and non-treated control, but plant mortality and plant mortality associated to M. phaseolina decreased by more than 24% and 65% respectively, in Hvs8 treatment. This study suggests that Brevibacterium sp. Hvs8 strain could be a candidate for controlling charcoal rot in strawberry. EEA Famaillá Fil: Viejobueno, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Rodríguez-Berbel, Natalia. University of Almeria. Department of Agronomy & Center for Intensive Mediterranean Agronomy and Agri-Food Biotechnology (CIAIMBITAL); España Fil: Miranda, Luis. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica; España Fil: Santos, Berta de los. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica; España Fil: Camacho, María. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica; España 2022-05-03T17:12:21Z 2022-05-03T17:12:21Z 2021-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11797 https://www.mdpi.com/2311-7524/7/11/457 2311-7524 https://doi.org/10.3390/horticulturae7110457 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Horticulturae 7 (11) : 457. (November 2021) |
| spellingShingle | Fresa Enfermedades de las Plantas Control de Enfermedades de Plantas Macrophomina phaseolina Control Biológico Bacterias Antagonistas Strawberries Plant Diseases Plant Disease Control Biological Control Antagonistic Bacteria Frutilla Charcoal Rot Disease Viejobueno, Josefina Rodríguez-Berbel, Natalia Miranda, Luis Santos, Berta de los Camacho, María Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry |
| title | Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry |
| title_full | Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry |
| title_fullStr | Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry |
| title_full_unstemmed | Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry |
| title_short | Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry |
| title_sort | potential bacterial antagonists for the control of charcoal rot macrophomina phaseolina in strawberry |
| topic | Fresa Enfermedades de las Plantas Control de Enfermedades de Plantas Macrophomina phaseolina Control Biológico Bacterias Antagonistas Strawberries Plant Diseases Plant Disease Control Biological Control Antagonistic Bacteria Frutilla Charcoal Rot Disease |
| url | http://hdl.handle.net/20.500.12123/11797 https://www.mdpi.com/2311-7524/7/11/457 https://doi.org/10.3390/horticulturae7110457 |
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