Pharmacologycal activity of peperina (Minthostachys verticillata) on gastrointestinal tract
Ethnopharmacological relevance: Minthostachys verticillata (Griseb.) Epling (Lamiaceae), known as Peperina is a medicinal native plant, with a traditional use as a digestive, antispasmodic and antidiarrheic. Aim Of The Study: Despite its folkloric use, no scientific evaluation of this plant relate...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/11761 https://www.sciencedirect.com/science/article/pii/S037887412033600X https://doi.org/10.1016/j.jep.2020.113712 |
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| author | Rodríguez Basso, Angeles Carranza, A. Zainutti, V.M. Bach, Hernán Gerónimo Gorzalczany, S.B. |
| author_browse | Bach, Hernán Gerónimo Carranza, A. Gorzalczany, S.B. Rodríguez Basso, Angeles Zainutti, V.M. |
| author_facet | Rodríguez Basso, Angeles Carranza, A. Zainutti, V.M. Bach, Hernán Gerónimo Gorzalczany, S.B. |
| author_sort | Rodríguez Basso, Angeles |
| collection | INTA Digital |
| description | Ethnopharmacological relevance: Minthostachys verticillata (Griseb.) Epling (Lamiaceae), known as Peperina is a medicinal native plant, with a traditional use as a digestive, antispasmodic and antidiarrheic.
Aim Of The Study: Despite its folkloric use, no scientific evaluation of this plant related to the astrointestinal inflammatory process has been carried out to date. The present study aims to assess the effects of M. verticillata on gastrointestinal system in experimental models.
Materials and methods: M. verticillata (250 and 500 mg/kg) was orally tested in a colitis model induced by acetic acid. Colon weight/length ratio, oxidative stress (oxidized and reduced glutathione), histological changes using Alcian blue and hematoxylin & eosin staining and expression of IL1β, TNFα, iNOS, COX-2 were evaluated. The effect of the extract in three additional in vivo models were studied: intestinal motility and diarrhea induced by ricin oil, and visceral pain induced by intracolonic administration of capsaicin. Finally, the activity on con centration response curves of acetylcholine, calcium chloride, potassium and serotonin were achieved in isolated rat jejunum.
Results: In the colitis model, M. verticillata induced a significant reduction in the colon weight/length ratio,
oxidative stress and expression levels of IL-1β, iNOS and COX-2. Also, the extract diminished the severity of microscopic tissue damage and showed protective effect on goblet cells. Intestinal motility, diarrhea, visceral pain-related behaviors and referred hyperalgesia were significantly reduced when the animals were treated with the extract. Furthermore, in isolated jejunum, M. verticillata significantly reduced the contraction induced by serotonin and acetylcholine. Likewise, the extract non-competitively inhibited the response-concentration induced by CaCl2 and inhibited both low and high K+-induced contractions.
Conclusions: This is the first study to validate traditional use of M. verticillata for digestive disorders and
demonstrated that its aqueous extract could represent a promising strategy in targeting the multifactorial
pathophysiology of inflammatory bowel disease. |
| format | Artículo |
| id | INTA11761 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA117612024-02-26T12:10:00Z Pharmacologycal activity of peperina (Minthostachys verticillata) on gastrointestinal tract Rodríguez Basso, Angeles Carranza, A. Zainutti, V.M. Bach, Hernán Gerónimo Gorzalczany, S.B. Minthostachys Peperina Minthostachys verticillata Ethnopharmacological relevance: Minthostachys verticillata (Griseb.) Epling (Lamiaceae), known as Peperina is a medicinal native plant, with a traditional use as a digestive, antispasmodic and antidiarrheic. Aim Of The Study: Despite its folkloric use, no scientific evaluation of this plant related to the astrointestinal inflammatory process has been carried out to date. The present study aims to assess the effects of M. verticillata on gastrointestinal system in experimental models. Materials and methods: M. verticillata (250 and 500 mg/kg) was orally tested in a colitis model induced by acetic acid. Colon weight/length ratio, oxidative stress (oxidized and reduced glutathione), histological changes using Alcian blue and hematoxylin & eosin staining and expression of IL1β, TNFα, iNOS, COX-2 were evaluated. The effect of the extract in three additional in vivo models were studied: intestinal motility and diarrhea induced by ricin oil, and visceral pain induced by intracolonic administration of capsaicin. Finally, the activity on con centration response curves of acetylcholine, calcium chloride, potassium and serotonin were achieved in isolated rat jejunum. Results: In the colitis model, M. verticillata induced a significant reduction in the colon weight/length ratio, oxidative stress and expression levels of IL-1β, iNOS and COX-2. Also, the extract diminished the severity of microscopic tissue damage and showed protective effect on goblet cells. Intestinal motility, diarrhea, visceral pain-related behaviors and referred hyperalgesia were significantly reduced when the animals were treated with the extract. Furthermore, in isolated jejunum, M. verticillata significantly reduced the contraction induced by serotonin and acetylcholine. Likewise, the extract non-competitively inhibited the response-concentration induced by CaCl2 and inhibited both low and high K+-induced contractions. Conclusions: This is the first study to validate traditional use of M. verticillata for digestive disorders and demonstrated that its aqueous extract could represent a promising strategy in targeting the multifactorial pathophysiology of inflammatory bowel disease. Fil: Rodríguez Basso, A. Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Catedra de Farmacología, Buenos Aires, Argentina Fil: Carranza, A. CONICET- Universidad de Buenos Aires. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional (IATIMET), Buenos Aires, Argentina Fil: Zainutti, V.M. Hospital Interzonal de Agudos “Evita”, Buenos Aires, Argentina Fil: Bach, Hernán Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Gorzalczany, S.B. Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Catedra de Farmacología, Buenos Aires, Argentina 2022-04-28T13:41:44Z 2022-04-28T13:41:44Z 2020-12-25 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11761 https://www.sciencedirect.com/science/article/pii/S037887412033600X 0378-8741 https://doi.org/10.1016/j.jep.2020.113712 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Journal of Ethnopharmacology 269 : 113712. (April 2021) |
| spellingShingle | Minthostachys Peperina Minthostachys verticillata Rodríguez Basso, Angeles Carranza, A. Zainutti, V.M. Bach, Hernán Gerónimo Gorzalczany, S.B. Pharmacologycal activity of peperina (Minthostachys verticillata) on gastrointestinal tract |
| title | Pharmacologycal activity of peperina (Minthostachys verticillata) on gastrointestinal tract |
| title_full | Pharmacologycal activity of peperina (Minthostachys verticillata) on gastrointestinal tract |
| title_fullStr | Pharmacologycal activity of peperina (Minthostachys verticillata) on gastrointestinal tract |
| title_full_unstemmed | Pharmacologycal activity of peperina (Minthostachys verticillata) on gastrointestinal tract |
| title_short | Pharmacologycal activity of peperina (Minthostachys verticillata) on gastrointestinal tract |
| title_sort | pharmacologycal activity of peperina minthostachys verticillata on gastrointestinal tract |
| topic | Minthostachys Peperina Minthostachys verticillata |
| url | http://hdl.handle.net/20.500.12123/11761 https://www.sciencedirect.com/science/article/pii/S037887412033600X https://doi.org/10.1016/j.jep.2020.113712 |
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