Incidence of Tetracycline and Erythromycin Resistance in Meat-Associated Bacteria: Impact of Different Livestock Management Strategies

The extensive use of antibiotics as growth promoters, or their continued abusive misuse to cure or prevent the onset of bacterial infections as occurs in the intensive farming, may have played a pivotal role in the spread of reservoirs of antibiotic resistance (AR) among food-associated bacteria inc...

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Autores principales: Fontana, Cecilia Alejandra, Patrone, Vania, López, Constanza María, Morelli, Lorenzo, Rebecchi, Annalisa
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: MDPI 2022
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/11751
https://www.mdpi.com/2076-2607/9/10/2111
https://doi.org/10.3390/microorganisms9102111
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author Fontana, Cecilia Alejandra
Patrone, Vania
López, Constanza María
Morelli, Lorenzo
Rebecchi, Annalisa
author_browse Fontana, Cecilia Alejandra
López, Constanza María
Morelli, Lorenzo
Patrone, Vania
Rebecchi, Annalisa
author_facet Fontana, Cecilia Alejandra
Patrone, Vania
López, Constanza María
Morelli, Lorenzo
Rebecchi, Annalisa
author_sort Fontana, Cecilia Alejandra
collection INTA Digital
description The extensive use of antibiotics as growth promoters, or their continued abusive misuse to cure or prevent the onset of bacterial infections as occurs in the intensive farming, may have played a pivotal role in the spread of reservoirs of antibiotic resistance (AR) among food-associated bacteria including pathogens representing risks to human health. The present study compares the incidence of tetracycline and erythromycin resistances in lactic acid bacteria (LAB) and coagulase negative staphylococci (CNS) from fermented products manufacturing using meat from intensive animal husbandry (industrialized manufacturing Italian salami) and from extensive farms (artisanal sausages facilities pork and llama Argentinean sausages). A higher incidence of tetracycline resistance (TET-R) compared to erythromycin resistance (ERY-R) was observed among the 205 isolates. Unlike CNS strains, the LAB showed a significant correlation between the TET-R and the ERY-R phenotypes. Genotypic assessment shows a high correlation with tetK and tetM for the TET-R strains and with ermB and ermC for the ERY-R strains. Multiple correspondence analyses have highlighted the association between AR phenotypes and CNS species isolated from Italian salami, while the susceptible phenotypes were associated with the LAB species from Argentinean sausages. Since antibiotic resistance in meat-associated bacteria is a very complex phenomenon, the assessment of bacterial resistance in different environmental contexts with diverse farming practices and food production technologies will help in monitoring the factors influencing AR emergence and spread in animal production.
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spelling INTA117512022-04-27T17:12:02Z Incidence of Tetracycline and Erythromycin Resistance in Meat-Associated Bacteria: Impact of Different Livestock Management Strategies Fontana, Cecilia Alejandra Patrone, Vania López, Constanza María Morelli, Lorenzo Rebecchi, Annalisa Tetraciclina Eritromicina Manejo del Ganado Resistencia a los Antibióticos Bacterias Acidolácticas Cerdo Llama Tetracyclines Erythromycin Livestock Management Resistance to Antibiotics Lactic Acid Bacteria Swine Llamas The extensive use of antibiotics as growth promoters, or their continued abusive misuse to cure or prevent the onset of bacterial infections as occurs in the intensive farming, may have played a pivotal role in the spread of reservoirs of antibiotic resistance (AR) among food-associated bacteria including pathogens representing risks to human health. The present study compares the incidence of tetracycline and erythromycin resistances in lactic acid bacteria (LAB) and coagulase negative staphylococci (CNS) from fermented products manufacturing using meat from intensive animal husbandry (industrialized manufacturing Italian salami) and from extensive farms (artisanal sausages facilities pork and llama Argentinean sausages). A higher incidence of tetracycline resistance (TET-R) compared to erythromycin resistance (ERY-R) was observed among the 205 isolates. Unlike CNS strains, the LAB showed a significant correlation between the TET-R and the ERY-R phenotypes. Genotypic assessment shows a high correlation with tetK and tetM for the TET-R strains and with ermB and ermC for the ERY-R strains. Multiple correspondence analyses have highlighted the association between AR phenotypes and CNS species isolated from Italian salami, while the susceptible phenotypes were associated with the LAB species from Argentinean sausages. Since antibiotic resistance in meat-associated bacteria is a very complex phenomenon, the assessment of bacterial resistance in different environmental contexts with diverse farming practices and food production technologies will help in monitoring the factors influencing AR emergence and spread in animal production. EEA Famaillá Fil: Fontana, Cecilia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Fontana, Cecilia Alejandra. Universita Cattolica del Sacro Cuore. Centro Ricerche Biotecnologiche; Italia Fil: Patrone, Vania. Università Cattolica del Sacro Cuore. DISTAS; Italia Fil: López, Constanza María. Universita Cattolica del Sacro Cuore. Centro Ricerche Biotecnologiche; Italia Fil: Morelli, Lorenzo. Universita Cattolica del Sacro Cuore. Centro Ricerche Biotecnologiche; Italia Fil: Morelli, Lorenzo. Università Cattolica del Sacro Cuore. DISTAS; Italia Fil: Rebecchi, Annalisa. Universita Cattolica del Sacro Cuore. Centro Ricerche Biotecnologiche; Italia Fil: Rebecchi, Annalisa. Università Cattolica del Sacro Cuore. DISTAS; Italia 2022-04-27T17:08:59Z 2022-04-27T17:08:59Z 2021-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11751 https://www.mdpi.com/2076-2607/9/10/2111 2076-2607 https://doi.org/10.3390/microorganisms9102111 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Microorganisms 9 (10) : 2111. (October 2021)
spellingShingle Tetraciclina
Eritromicina
Manejo del Ganado
Resistencia a los Antibióticos
Bacterias Acidolácticas
Cerdo
Llama
Tetracyclines
Erythromycin
Livestock Management
Resistance to Antibiotics
Lactic Acid Bacteria
Swine
Llamas
Fontana, Cecilia Alejandra
Patrone, Vania
López, Constanza María
Morelli, Lorenzo
Rebecchi, Annalisa
Incidence of Tetracycline and Erythromycin Resistance in Meat-Associated Bacteria: Impact of Different Livestock Management Strategies
title Incidence of Tetracycline and Erythromycin Resistance in Meat-Associated Bacteria: Impact of Different Livestock Management Strategies
title_full Incidence of Tetracycline and Erythromycin Resistance in Meat-Associated Bacteria: Impact of Different Livestock Management Strategies
title_fullStr Incidence of Tetracycline and Erythromycin Resistance in Meat-Associated Bacteria: Impact of Different Livestock Management Strategies
title_full_unstemmed Incidence of Tetracycline and Erythromycin Resistance in Meat-Associated Bacteria: Impact of Different Livestock Management Strategies
title_short Incidence of Tetracycline and Erythromycin Resistance in Meat-Associated Bacteria: Impact of Different Livestock Management Strategies
title_sort incidence of tetracycline and erythromycin resistance in meat associated bacteria impact of different livestock management strategies
topic Tetraciclina
Eritromicina
Manejo del Ganado
Resistencia a los Antibióticos
Bacterias Acidolácticas
Cerdo
Llama
Tetracyclines
Erythromycin
Livestock Management
Resistance to Antibiotics
Lactic Acid Bacteria
Swine
Llamas
url http://hdl.handle.net/20.500.12123/11751
https://www.mdpi.com/2076-2607/9/10/2111
https://doi.org/10.3390/microorganisms9102111
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