Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress
Although sunflower (Helianthus annuus L.) is categorized as a medium drought-sensitive crop, in a changing climate scenario and/or with the onset of early droughts, the crop may be affected by water stress. This study characterizes and compares the gene expression profiles between aerial part and ro...
| Main Authors: | , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2022
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/11725 https://link.springer.com/article/10.1007/s11105-020-01192-4 https://doi.org/10.1007/s11105-020-01192-4 |
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| author | Escalante, Maximiliano Vigliocco, Ana Edit Moschen, Sebastian Nicolas Fernandez, Paula Del Carmen Heinz, Ruth Amelia Garcia-Garcia, Francisco Di Rienzo, Julio A. Andrade, Andrea Alemano, Sergio Gabriel |
| author_browse | Alemano, Sergio Gabriel Andrade, Andrea Di Rienzo, Julio A. Escalante, Maximiliano Fernandez, Paula Del Carmen Garcia-Garcia, Francisco Heinz, Ruth Amelia Moschen, Sebastian Nicolas Vigliocco, Ana Edit |
| author_facet | Escalante, Maximiliano Vigliocco, Ana Edit Moschen, Sebastian Nicolas Fernandez, Paula Del Carmen Heinz, Ruth Amelia Garcia-Garcia, Francisco Di Rienzo, Julio A. Andrade, Andrea Alemano, Sergio Gabriel |
| author_sort | Escalante, Maximiliano |
| collection | INTA Digital |
| description | Although sunflower (Helianthus annuus L.) is categorized as a medium drought-sensitive crop, in a changing climate scenario and/or with the onset of early droughts, the crop may be affected by water stress. This study characterizes and compares the gene expression profiles between aerial part and roots of two sunflower inbred lines with contrasting response to water stress: B59 (sensitive) and B71 (tolerant) under non-stress and water stress. Microarray analysis revealed that water stress induced significant changes in gene expression of both lines. The B59 line had a higher number of genes differentially expressed in water-stressed seedlings compared with B71 line. In both lines, most of the water stress responding genes was upregulated. In B59, the number of genes specifically upregulated in aerial part was higher than that observed in B71. In roots, B71 had more upregulated genes compared with B59. Genes involved in signaling pathway of hormones, components of redox system, and secondary metabolites were enriched in both organs of two lines. The knowledge generated could be helpful to provide tools, that together with the genetic engineering and molecular breeding, it would contribute for the development of water stress-tolerant varieties in crop plants. |
| format | info:ar-repo/semantics/artículo |
| id | INTA11725 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA117252022-08-12T16:21:10Z Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress Escalante, Maximiliano Vigliocco, Ana Edit Moschen, Sebastian Nicolas Fernandez, Paula Del Carmen Heinz, Ruth Amelia Garcia-Garcia, Francisco Di Rienzo, Julio A. Andrade, Andrea Alemano, Sergio Gabriel Girasol Helianthus annuus Expresión Génica Estrés de Sequia Lineas Consanguíneas Sunflowers Gene Expression Profiling Gene Expression Drought Stress Inbred Lines Water Stress Although sunflower (Helianthus annuus L.) is categorized as a medium drought-sensitive crop, in a changing climate scenario and/or with the onset of early droughts, the crop may be affected by water stress. This study characterizes and compares the gene expression profiles between aerial part and roots of two sunflower inbred lines with contrasting response to water stress: B59 (sensitive) and B71 (tolerant) under non-stress and water stress. Microarray analysis revealed that water stress induced significant changes in gene expression of both lines. The B59 line had a higher number of genes differentially expressed in water-stressed seedlings compared with B71 line. In both lines, most of the water stress responding genes was upregulated. In B59, the number of genes specifically upregulated in aerial part was higher than that observed in B71. In roots, B71 had more upregulated genes compared with B59. Genes involved in signaling pathway of hormones, components of redox system, and secondary metabolites were enriched in both organs of two lines. The knowledge generated could be helpful to provide tools, that together with the genetic engineering and molecular breeding, it would contribute for the development of water stress-tolerant varieties in crop plants. Instituto de Biotecnología Fil: Escalante, Maximiliano. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; Argentina Fil: Vigliocco, Ana E. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; Argentina Fil: Moschen, Sebastian Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Moschen, Sebastian Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Fernandez, Paula Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fernandez, Paula Del Carmen. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Heinz, Ruth Amelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Heinz, Ruth Amelia. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: García-García, Francisco. Centro de Investigación Príncipe Felipe. Department of Bioinformatics and Genomics; España. Fil: Di Rienzo, Julio A. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina Fil: Andrade, Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; Argentina Fil: Andrade, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alemano, Sergio. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; Argentina 2022-04-25T15:08:18Z 2022-04-25T15:08:18Z 2020-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11725 https://link.springer.com/article/10.1007/s11105-020-01192-4 0735-9640 1572-9818 https://doi.org/10.1007/s11105-020-01192-4 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Plant Molecular Biology Reporter 38 : 222–237 (2020) |
| spellingShingle | Girasol Helianthus annuus Expresión Génica Estrés de Sequia Lineas Consanguíneas Sunflowers Gene Expression Profiling Gene Expression Drought Stress Inbred Lines Water Stress Escalante, Maximiliano Vigliocco, Ana Edit Moschen, Sebastian Nicolas Fernandez, Paula Del Carmen Heinz, Ruth Amelia Garcia-Garcia, Francisco Di Rienzo, Julio A. Andrade, Andrea Alemano, Sergio Gabriel Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress |
| title | Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress |
| title_full | Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress |
| title_fullStr | Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress |
| title_full_unstemmed | Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress |
| title_short | Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress |
| title_sort | transcriptomic analysis reveals a differential gene expression profile between two sunflower inbred lines with different ability to tolerate water stress |
| topic | Girasol Helianthus annuus Expresión Génica Estrés de Sequia Lineas Consanguíneas Sunflowers Gene Expression Profiling Gene Expression Drought Stress Inbred Lines Water Stress |
| url | http://hdl.handle.net/20.500.12123/11725 https://link.springer.com/article/10.1007/s11105-020-01192-4 https://doi.org/10.1007/s11105-020-01192-4 |
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