Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower
The nature of non-target-site herbicide resistance (NTSR) to imidazolinone (IMI) in HA425 sunflower (Helianthus annuus L.) has not yet been fully characterized but could be related to xenobiotic metabolism. The objective of this study was to evaluate the role of cytochrome P450 monooxygenases (P450s...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Wiley
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/11724 https://acsess.onlinelibrary.wiley.com/doi/abs/10.1002/csc2.20138 https://doi.org/10.1002/csc2.20138 |
| _version_ | 1855484837208522752 |
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| author | Vega, Tatiana Gil, Mercedes Martin, Gabriela Moschen, Sebastian Nicolas Picardi, Liliana Nestares, Graciela |
| author_browse | Gil, Mercedes Martin, Gabriela Moschen, Sebastian Nicolas Nestares, Graciela Picardi, Liliana Vega, Tatiana |
| author_facet | Vega, Tatiana Gil, Mercedes Martin, Gabriela Moschen, Sebastian Nicolas Picardi, Liliana Nestares, Graciela |
| author_sort | Vega, Tatiana |
| collection | INTA Digital |
| description | The nature of non-target-site herbicide resistance (NTSR) to imidazolinone (IMI) in HA425 sunflower (Helianthus annuus L.) has not yet been fully characterized but could be related to xenobiotic metabolism. The objective of this study was to evaluate the role of cytochrome P450 monooxygenases (P450s) and other detoxification-related proteins in NTSR in sunflower. Two sunflower inbred lines were used: HA 425, which is IMI resistant (Imisun), and HA 89, which is IMI susceptible. The growth response to the IMI herbicide imazethapyr in combination with the P450 inhibitors 1-aminobenzotriazole (ABT) or piperonyl butoxide (PBO) was evaluated in 15-d-old sunflower plantlets. Roots were collected, and label-free quantitation (LFQ) proteomic analysis was carried out to characterize the NTSR mechanisms involved in the IMI resistance trait in sunflower. The increased phytotoxicity of imazethapyr observed in the resistant line when ABT or PBO were present agrees with the hypothesis that NTSR mechanisms may contribute to herbicide resistance in sunflower. The herbicide treatment also led to changes in the levels of biotic and abiotic stress-related proteins, glutathione S-transferases, and cytochrome P450s, among others. Plant growth and root protein expression response to IMI herbicides in sunflower would be a combination of stress-related and detoxification mechanisms. Understanding the basis of NTSR becomes helpful to exploit this trait in sunflower crop and to develop xenobiotic-resistant, soil-remediating cultivars. |
| format | Artículo |
| id | INTA11724 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA117242022-04-25T14:34:46Z Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower Vega, Tatiana Gil, Mercedes Martin, Gabriela Moschen, Sebastian Nicolas Picardi, Liliana Nestares, Graciela Girasol Herbicidas Resistencia a los Herbicidas Estres Desintoxicación Helianthus annuus Sunflowers Herbicides Herbicide Resistance Stress Detoxification The nature of non-target-site herbicide resistance (NTSR) to imidazolinone (IMI) in HA425 sunflower (Helianthus annuus L.) has not yet been fully characterized but could be related to xenobiotic metabolism. The objective of this study was to evaluate the role of cytochrome P450 monooxygenases (P450s) and other detoxification-related proteins in NTSR in sunflower. Two sunflower inbred lines were used: HA 425, which is IMI resistant (Imisun), and HA 89, which is IMI susceptible. The growth response to the IMI herbicide imazethapyr in combination with the P450 inhibitors 1-aminobenzotriazole (ABT) or piperonyl butoxide (PBO) was evaluated in 15-d-old sunflower plantlets. Roots were collected, and label-free quantitation (LFQ) proteomic analysis was carried out to characterize the NTSR mechanisms involved in the IMI resistance trait in sunflower. The increased phytotoxicity of imazethapyr observed in the resistant line when ABT or PBO were present agrees with the hypothesis that NTSR mechanisms may contribute to herbicide resistance in sunflower. The herbicide treatment also led to changes in the levels of biotic and abiotic stress-related proteins, glutathione S-transferases, and cytochrome P450s, among others. Plant growth and root protein expression response to IMI herbicides in sunflower would be a combination of stress-related and detoxification mechanisms. Understanding the basis of NTSR becomes helpful to exploit this trait in sunflower crop and to develop xenobiotic-resistant, soil-remediating cultivars. EEA Famaillá Fil: Vega, Tatiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias Agrarias; Argentina Fil: Vega, Tatiana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias; Argentina Fil: Gil, Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias Agrarias; Argentina Fil: Gil, Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias; Argentina Fil: Martin, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias Agrarias; Argentina Fil: Martin, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias; Argentina Fil: Moschen, Sebastian Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina. Fil: Moschen, Sebastian Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Picardi, Liliana. Universidad Nacional de Rosario. Consejo de Investigaciones; Argentina Fil: Nestares, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias Agrarias; Argentina Fil: Nestares, Graciela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias; Argentina 2022-04-25T14:29:49Z 2022-04-25T14:29:49Z 2020-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11724 https://acsess.onlinelibrary.wiley.com/doi/abs/10.1002/csc2.20138 1435-0653 https://doi.org/10.1002/csc2.20138 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Crop Science 60 (4) : 1809-1822 (July-August 2020) |
| spellingShingle | Girasol Herbicidas Resistencia a los Herbicidas Estres Desintoxicación Helianthus annuus Sunflowers Herbicides Herbicide Resistance Stress Detoxification Vega, Tatiana Gil, Mercedes Martin, Gabriela Moschen, Sebastian Nicolas Picardi, Liliana Nestares, Graciela Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower |
| title | Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower |
| title_full | Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower |
| title_fullStr | Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower |
| title_full_unstemmed | Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower |
| title_short | Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower |
| title_sort | stress response and detoxification mechanisms involved in non target site herbicide resistance in sunflower |
| topic | Girasol Herbicidas Resistencia a los Herbicidas Estres Desintoxicación Helianthus annuus Sunflowers Herbicides Herbicide Resistance Stress Detoxification |
| url | http://hdl.handle.net/20.500.12123/11724 https://acsess.onlinelibrary.wiley.com/doi/abs/10.1002/csc2.20138 https://doi.org/10.1002/csc2.20138 |
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