A reference chromosomal map of the hot chili pepper Capsicum pubescens cv. “locoto” (Solanaceae)
Capsicum pubescens is a cultivated hot chili pepper, consumed in Latin American cuisine as a distinctive ingredient, and popularly known as “locoto” or “rocoto”. This taxon is also an outstanding source of resistance to biotic and abiotic stresses as well as other valuable fruit traits for breedin...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Instituto de Pesquisas Jardim Botânico do Rio de Janeiro
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/11613 https://www.scielo.br/j/rod/a/LLVygxjK7DwpmzJTWpNKmSm/?format=pdf&lang=en https://doi.org/10.1590/2175-7860202172062 |
| _version_ | 1855036605147906048 |
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| author | Aguilera, Patricia Mabel Debat, Humberto Julio Seijo, José Guillermo Grabiele, Mauro |
| author_browse | Aguilera, Patricia Mabel Debat, Humberto Julio Grabiele, Mauro Seijo, José Guillermo |
| author_facet | Aguilera, Patricia Mabel Debat, Humberto Julio Seijo, José Guillermo Grabiele, Mauro |
| author_sort | Aguilera, Patricia Mabel |
| collection | INTA Digital |
| description | Capsicum pubescens is a cultivated hot chili pepper, consumed in Latin American cuisine as a distinctive
ingredient, and popularly known as “locoto” or “rocoto”. This taxon is also an outstanding source of
resistance to biotic and abiotic stresses as well as other valuable fruit traits for breeding of the worldwidely cultivated C. annuum and related species. In this study, the chromosome complement of C. pubescens cv. “locoto” (2n = 24) was deeply characterized through a sequential combination of conventional and molecular cytogenetics approaches comprising: Ag-NOR staining, heterochromatic fluorescent C-DAPI, DAPI/AMDCMA/DA bandings, fluorescence in situ hybridization (FISH) of Capsicum-derived probes of the 5S and 18S-25S rRNA genes and different regions of spacers of the ribosomal unit, as well as telomeric probe. The markers identified were systematically combined with morphological karyotype parameters - number, size, centromeres, satellites - to produce a physical map which allowed the identification of several landmarks in each individual chromosome. The reference chromosomal map of C. pubescens here presented is the most
comprehensively developed in Capsicum so far. It is envisioned that this chromosomal map will serve as
a reference framework for the upcoming sequencing projects and as starting point to assist future genetic
mapping of important agronomic traits. |
| format | info:ar-repo/semantics/artículo |
| id | INTA11613 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Instituto de Pesquisas Jardim Botânico do Rio de Janeiro |
| publisherStr | Instituto de Pesquisas Jardim Botânico do Rio de Janeiro |
| record_format | dspace |
| spelling | INTA116132022-04-11T11:03:59Z A reference chromosomal map of the hot chili pepper Capsicum pubescens cv. “locoto” (Solanaceae) Aguilera, Patricia Mabel Debat, Humberto Julio Seijo, José Guillermo Grabiele, Mauro Heterochromatin Chillies Heterocromatina Capsicum Capsicum pubescens Guindilla Ag-NOR Locoto Bandeos Fluorescentes Rocoto Ají Fluorescent Bandings rDNA FISH Capsicum pubescens is a cultivated hot chili pepper, consumed in Latin American cuisine as a distinctive ingredient, and popularly known as “locoto” or “rocoto”. This taxon is also an outstanding source of resistance to biotic and abiotic stresses as well as other valuable fruit traits for breeding of the worldwidely cultivated C. annuum and related species. In this study, the chromosome complement of C. pubescens cv. “locoto” (2n = 24) was deeply characterized through a sequential combination of conventional and molecular cytogenetics approaches comprising: Ag-NOR staining, heterochromatic fluorescent C-DAPI, DAPI/AMDCMA/DA bandings, fluorescence in situ hybridization (FISH) of Capsicum-derived probes of the 5S and 18S-25S rRNA genes and different regions of spacers of the ribosomal unit, as well as telomeric probe. The markers identified were systematically combined with morphological karyotype parameters - number, size, centromeres, satellites - to produce a physical map which allowed the identification of several landmarks in each individual chromosome. The reference chromosomal map of C. pubescens here presented is the most comprehensively developed in Capsicum so far. It is envisioned that this chromosomal map will serve as a reference framework for the upcoming sequencing projects and as starting point to assist future genetic mapping of important agronomic traits. Capsicum pubescens es un ají picante cultivado, consumido en Latinoamérica como ingrediente distintivo y conocido como “locoto” o “rocoto”. Este taxón es un recurso sobresaliente por sus características de resistencia y tolerancia a estrés biótico/abiótico, y del fruto, que pueden ser introgresadas en programas de mejoramiento de C. annuum y especies relacionadas. En este estudio, se caracterizó detalladamente el complemento cromosómico de C. pubescens cv. “locoto” (2n = 24) a través de una combinación secuencial de herramientas citogenéticas convencionales y moleculares que incluyeron: tinción Ag-NOR, bandeos heterocromáticos fluorescentes C-DAPI, DAPI/AMD-CMA/DA, e hibridación in situ fluorescente (FISH) con sondas de genes de rRNA 5S y 18S-25S y diferentes regiones espaciadoras de la unidad ribosomal derivadas de Capsicum, y sonda telomérica. Los marcadores identificados se combinaron sistemáticamente con parámetros morfométricos del cariotipo - número, tamaño, centrómeros, satélites - para producir un mapa físico que permitió la identificación de varios caracteres de referencia en cada cromosoma individual. El mapa cromosómico de referencia de C. pubescens aquí presentado es el más completo hasta ahora desarrollado en Capsicum. Se prevé que este mapa cromosómico sirva de referencia para próximos proyectos de secuenciación y como punto de partida para asistir al mapeo genético de caracteres agronómicos importantes. Instituto de Patología Vegetal Fil: Aguilera, Patricia Mabel. Universidad Nacional de Misiones, Instituto de Biología Subtropical. Instituto de Biotecnología Misiones; Argentina Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Seijo, José Guillermo. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina Fil: Grabiele, Mauro. Universidad Nacional de Misiones, Instituto de Biología Subtropical. Instituto de Biotecnología Misiones; Argentina 2022-04-11T10:56:06Z 2022-04-11T10:56:06Z 2021 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11613 https://www.scielo.br/j/rod/a/LLVygxjK7DwpmzJTWpNKmSm/?format=pdf&lang=en 0370-6583 2175-7860 https://doi.org/10.1590/2175-7860202172062 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Instituto de Pesquisas Jardim Botânico do Rio de Janeiro Rodriguésia 72 : e01002020 (2021) |
| spellingShingle | Heterochromatin Chillies Heterocromatina Capsicum Capsicum pubescens Guindilla Ag-NOR Locoto Bandeos Fluorescentes Rocoto Ají Fluorescent Bandings rDNA FISH Aguilera, Patricia Mabel Debat, Humberto Julio Seijo, José Guillermo Grabiele, Mauro A reference chromosomal map of the hot chili pepper Capsicum pubescens cv. “locoto” (Solanaceae) |
| title | A reference chromosomal map of the hot chili pepper Capsicum pubescens cv. “locoto” (Solanaceae) |
| title_full | A reference chromosomal map of the hot chili pepper Capsicum pubescens cv. “locoto” (Solanaceae) |
| title_fullStr | A reference chromosomal map of the hot chili pepper Capsicum pubescens cv. “locoto” (Solanaceae) |
| title_full_unstemmed | A reference chromosomal map of the hot chili pepper Capsicum pubescens cv. “locoto” (Solanaceae) |
| title_short | A reference chromosomal map of the hot chili pepper Capsicum pubescens cv. “locoto” (Solanaceae) |
| title_sort | reference chromosomal map of the hot chili pepper capsicum pubescens cv locoto solanaceae |
| topic | Heterochromatin Chillies Heterocromatina Capsicum Capsicum pubescens Guindilla Ag-NOR Locoto Bandeos Fluorescentes Rocoto Ají Fluorescent Bandings rDNA FISH |
| url | http://hdl.handle.net/20.500.12123/11613 https://www.scielo.br/j/rod/a/LLVygxjK7DwpmzJTWpNKmSm/?format=pdf&lang=en https://doi.org/10.1590/2175-7860202172062 |
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