Molecular characterization of the 5S rDNA non-transcribed spacer and reconstruction of phylogenetic relationships in Capsicum
Capsicum includes ca. 41 species of chili peppers. In this original report we PCR amplified, cloned, sequenced and characterized the 5S rDNA non-transcribed spacer -NTS- in 23 taxa of nine clades of Capsicum, divergent at geographical origin and fruit and chromosome traits, and compared the NTS feat...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Instituto de Pesquisas Jardim Botânico do Rio de Janeiro
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/11612 https://www.scielo.br/j/rod/a/mKbrh6B3GsMRvJj3cf9fVFL/?format=pdf&lang=en https://doi.org/10.1590/2175-7860202172071 |
| _version_ | 1855484815482028032 |
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| author | Grabiele, Mauro Aguilera, Patricia Mabel Ducasse, Daniel Adrian Debat, Humberto Julio |
| author_browse | Aguilera, Patricia Mabel Debat, Humberto Julio Ducasse, Daniel Adrian Grabiele, Mauro |
| author_facet | Grabiele, Mauro Aguilera, Patricia Mabel Ducasse, Daniel Adrian Debat, Humberto Julio |
| author_sort | Grabiele, Mauro |
| collection | INTA Digital |
| description | Capsicum includes ca. 41 species of chili peppers. In this original report we PCR amplified, cloned, sequenced and characterized the 5S rDNA non-transcribed spacer -NTS- in 23 taxa of nine clades of Capsicum, divergent at geographical origin and fruit and chromosome traits, and compared the NTS features throughout Solanaceae. According to GC content, inner variability and regulatory elements, the NTS organizes into three distinct structural regions; genetic variability at the NTS in Capsicum and related genus clusters into defined taxa hierarchies. Based on the reconstruction of a maximum-likelihood phylogenetic tree and phylogenetic networks, NTS sequences of Capsicum and related taxa grouped into well recognized categories -genus, section, clade,
species, variety-. An evolutionary scenario arose from combined genetic and phylogenetic NTS data, in
which monophyly and lineage diversification over time of Capsicum are addressed. Our analysis is original to include all domesticated species of Capsicum prevailing in germplasm collections and breeding programs, together with a large group of wild taxa that demanded further genetic characterization. The NTS set up as a double purpose marker in Capsicum, to directly evaluate genetic variability and reconstruct phylogenetic relationships to a broad extent, and constitutes a valuable tool for germplasm characterization and evolutionary studies within Solanaceae. |
| format | Artículo |
| id | INTA11612 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Instituto de Pesquisas Jardim Botânico do Rio de Janeiro |
| publisherStr | Instituto de Pesquisas Jardim Botânico do Rio de Janeiro |
| record_format | dspace |
| spelling | INTA116122022-04-11T10:52:18Z Molecular characterization of the 5S rDNA non-transcribed spacer and reconstruction of phylogenetic relationships in Capsicum Grabiele, Mauro Aguilera, Patricia Mabel Ducasse, Daniel Adrian Debat, Humberto Julio Chillies Capsicum Annuum Variación Genética Filogenia Genetic Variation Phylogeny Guindilla Chili Peppers Genetic Variability Molecular Double Purpose Marker Ribosomal NTS Ajíes Variabilidad Genética Marcador Molecular de Doble Propósito NTS Ribosómico Capsicum includes ca. 41 species of chili peppers. In this original report we PCR amplified, cloned, sequenced and characterized the 5S rDNA non-transcribed spacer -NTS- in 23 taxa of nine clades of Capsicum, divergent at geographical origin and fruit and chromosome traits, and compared the NTS features throughout Solanaceae. According to GC content, inner variability and regulatory elements, the NTS organizes into three distinct structural regions; genetic variability at the NTS in Capsicum and related genus clusters into defined taxa hierarchies. Based on the reconstruction of a maximum-likelihood phylogenetic tree and phylogenetic networks, NTS sequences of Capsicum and related taxa grouped into well recognized categories -genus, section, clade, species, variety-. An evolutionary scenario arose from combined genetic and phylogenetic NTS data, in which monophyly and lineage diversification over time of Capsicum are addressed. Our analysis is original to include all domesticated species of Capsicum prevailing in germplasm collections and breeding programs, together with a large group of wild taxa that demanded further genetic characterization. The NTS set up as a double purpose marker in Capsicum, to directly evaluate genetic variability and reconstruct phylogenetic relationships to a broad extent, and constitutes a valuable tool for germplasm characterization and evolutionary studies within Solanaceae. Capsicum incluye ca. 41 especies de ajíes. En este trabajo original, el espaciador no-transcrito (NTS) del ADNr 5S fue PCR-amplificado, clonado, secuenciado y caracterizado en 23 taxones de nueve clonados de Capsicum, divergentes en origen, fruto y cromosomas, y comparado a lo largo de Solanaceae. El NTS se organiza en tres regiones estructurales distintas de acuerdo a contenido GC, variabilidad y elementos reguladores; la variabilidad genética del NTS en Capsicum y géneros relacionados se agrupó en categorías taxonómicas definidas. Las secuencias NTS de Capsicum y taxa relacionados también se agruparon en categorías reconocidas -género, sección, clonado, especie, variedad- durante la reconstrucción de un árbol filogenético de máxima-verosimilitud y diversas redes filogenéticas. De la combinación de datos genéticos y filogenéticos del NTS surge un escenario evolutivo que considera monofilia y diversificación de Capsicum a lo largo del tiempo. Nuestro análisis es original al incluir todas las especies domesticadas de Capsicum, mayoritarias en colecciones y programas, además de un amplio número de ajíes silvestres que demandaban mayor caracterización genética. El NTS constituye un marcador de doble propósito en Capsicum, al evaluar directamente variabilidad genética y reconstruir relaciones filogenéticas extensas, además de ser útil a la caracterización de germoplasma y estudios evolutivos en Solanaceae. Instituto de Patología Vegetal Fil: Grabiele, Mauro. Universidad Nacional de Misiones, Instituto de Biología Subtropical. Instituto de Biotecnología Misiones; Argentina Fil: Aguilera, Patricia Mabel. Universidad Nacional de Misiones, Instituto de Biología Subtropical. Instituto de Biotecnología Misiones; Argentina Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2022-04-11T10:39:29Z 2022-04-11T10:39:29Z 2021 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11612 https://www.scielo.br/j/rod/a/mKbrh6B3GsMRvJj3cf9fVFL/?format=pdf&lang=en 0370-6583 2175-7860 https://doi.org/10.1590/2175-7860202172071 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Instituto de Pesquisas Jardim Botânico do Rio de Janeiro Rodriguésia 72 : e02062019 (2021) |
| spellingShingle | Chillies Capsicum Annuum Variación Genética Filogenia Genetic Variation Phylogeny Guindilla Chili Peppers Genetic Variability Molecular Double Purpose Marker Ribosomal NTS Ajíes Variabilidad Genética Marcador Molecular de Doble Propósito NTS Ribosómico Grabiele, Mauro Aguilera, Patricia Mabel Ducasse, Daniel Adrian Debat, Humberto Julio Molecular characterization of the 5S rDNA non-transcribed spacer and reconstruction of phylogenetic relationships in Capsicum |
| title | Molecular characterization of the 5S rDNA non-transcribed spacer and reconstruction of phylogenetic relationships in Capsicum |
| title_full | Molecular characterization of the 5S rDNA non-transcribed spacer and reconstruction of phylogenetic relationships in Capsicum |
| title_fullStr | Molecular characterization of the 5S rDNA non-transcribed spacer and reconstruction of phylogenetic relationships in Capsicum |
| title_full_unstemmed | Molecular characterization of the 5S rDNA non-transcribed spacer and reconstruction of phylogenetic relationships in Capsicum |
| title_short | Molecular characterization of the 5S rDNA non-transcribed spacer and reconstruction of phylogenetic relationships in Capsicum |
| title_sort | molecular characterization of the 5s rdna non transcribed spacer and reconstruction of phylogenetic relationships in capsicum |
| topic | Chillies Capsicum Annuum Variación Genética Filogenia Genetic Variation Phylogeny Guindilla Chili Peppers Genetic Variability Molecular Double Purpose Marker Ribosomal NTS Ajíes Variabilidad Genética Marcador Molecular de Doble Propósito NTS Ribosómico |
| url | http://hdl.handle.net/20.500.12123/11612 https://www.scielo.br/j/rod/a/mKbrh6B3GsMRvJj3cf9fVFL/?format=pdf&lang=en https://doi.org/10.1590/2175-7860202172071 |
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