Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
This study investigated terpene biosynthesis in different tissues (root, protobulb, leaf sheath and blade) of in vitro -grown garlic plants either infected or not (control) with Sclerotium cepivorum, the causative agent of Allium White Rot disease. The terpenes identified by gas chromatography–elect...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1161 http://ri.conicet.gov.ar/handle/11336/22405 http://www.sciencedirect.com/science/article/pii/S0031942215000503?via%3Dihub https://doi.org/10.1016/j.phytochem.2015.02.003 |
| _version_ | 1855482838939336704 |
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| author | Pontin, Mariela Ana Piccoli, Patricia Noemí Burba, Jose Luis Bottini, Ambrosio Rubén |
| author_browse | Bottini, Ambrosio Rubén Burba, Jose Luis Piccoli, Patricia Noemí Pontin, Mariela Ana |
| author_facet | Pontin, Mariela Ana Piccoli, Patricia Noemí Burba, Jose Luis Bottini, Ambrosio Rubén |
| author_sort | Pontin, Mariela Ana |
| collection | INTA Digital |
| description | This study investigated terpene biosynthesis in different tissues (root, protobulb, leaf sheath and blade) of in vitro -grown garlic plants either infected or not (control) with Sclerotium cepivorum, the causative agent of Allium White Rot disease. The terpenes identified by gas chromatography–electron impact mass spectrometry (GC-EIMS) in infected plants were nerolidol, phytol, squalene, a-pinene, terpinolene, limo-nene, 1,8-cineole and c-terpinene, whose levels significantly increased when exposed to the fungus. Consistent with this, an increase in terpene synthase (TPS) activity was measured in infected plants. Among the terpenes identified, nerolidol, a-pinene and terpinolene were the most abundant with antifungal activity against S. cepivorum being assessed in vitro by mycelium growth inhibition. Nerolidol and terpinolene significantly reduced sclerotia production, while a-pinene stimulated it in a concentration-dependent manner. Parallel to fungal growth inhibition, electron microscopy observations
established morphological alterations in the hyphae exposed to terpinolene and nerolidol. Differences in hyphal EtBr uptake suggested that one of the antifungal mechanisms of nerolidol and terpinolene migh be disruption of fungal membrane integrity |
| format | Artículo |
| id | INTA1161 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA11612018-02-20T15:44:39Z Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum Pontin, Mariela Ana Piccoli, Patricia Noemí Burba, Jose Luis Bottini, Ambrosio Rubén Ecología de la planta Allium Sativum Ajo Terpenoidos Sclerotium Cepivorum Propiedades Antimicósicas Garlic Terpenoids Antifungal Properties Terpenos This study investigated terpene biosynthesis in different tissues (root, protobulb, leaf sheath and blade) of in vitro -grown garlic plants either infected or not (control) with Sclerotium cepivorum, the causative agent of Allium White Rot disease. The terpenes identified by gas chromatography–electron impact mass spectrometry (GC-EIMS) in infected plants were nerolidol, phytol, squalene, a-pinene, terpinolene, limo-nene, 1,8-cineole and c-terpinene, whose levels significantly increased when exposed to the fungus. Consistent with this, an increase in terpene synthase (TPS) activity was measured in infected plants. Among the terpenes identified, nerolidol, a-pinene and terpinolene were the most abundant with antifungal activity against S. cepivorum being assessed in vitro by mycelium growth inhibition. Nerolidol and terpinolene significantly reduced sclerotia production, while a-pinene stimulated it in a concentration-dependent manner. Parallel to fungal growth inhibition, electron microscopy observations established morphological alterations in the hyphae exposed to terpinolene and nerolidol. Differences in hyphal EtBr uptake suggested that one of the antifungal mechanisms of nerolidol and terpinolene migh be disruption of fungal membrane integrity EEA La Consulta Fil: Pontin, Mariela Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Burba, José Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina Fil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina 2017-09-07T12:46:16Z 2017-09-07T12:46:16Z 2015-07 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion info:ar-repo/semantics/artículo http://hdl.handle.net/20.500.12123/1161 http://ri.conicet.gov.ar/handle/11336/22405 http://www.sciencedirect.com/science/article/pii/S0031942215000503?via%3Dihub 0031-9422 (Print) 1873-3700 (Online) https://doi.org/10.1016/j.phytochem.2015.02.003 eng info:eu-repo/semantics/restrictedAccess application/pdf Mendoza (province) Phytochemistry 115 :152-160. (July 2015) |
| spellingShingle | Ecología de la planta Allium Sativum Ajo Terpenoidos Sclerotium Cepivorum Propiedades Antimicósicas Garlic Terpenoids Antifungal Properties Terpenos Pontin, Mariela Ana Piccoli, Patricia Noemí Burba, Jose Luis Bottini, Ambrosio Rubén Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum |
| title | Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum |
| title_full | Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum |
| title_fullStr | Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum |
| title_full_unstemmed | Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum |
| title_short | Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum |
| title_sort | allium sativum produces terpenes with fungistatic properties in response to infection with sclerotium cepivorum |
| topic | Ecología de la planta Allium Sativum Ajo Terpenoidos Sclerotium Cepivorum Propiedades Antimicósicas Garlic Terpenoids Antifungal Properties Terpenos |
| url | http://hdl.handle.net/20.500.12123/1161 http://ri.conicet.gov.ar/handle/11336/22405 http://www.sciencedirect.com/science/article/pii/S0031942215000503?via%3Dihub https://doi.org/10.1016/j.phytochem.2015.02.003 |
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