Generation of a multi‑herbicide‑tolerant alfalfa by using base editing
We present the first report on base editing in alfalfa. Specifically, we showed edited alfalfa with tolerance to both sulfonylurea- and imidazolinone-type herbicides. Alfalfa (Medicago sativa) is one of the most important forage legumes worldwide. However, alfalfa-breeding programs are limited by th...
| Main Authors: | , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Springer
2022
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/11592 https://link.springer.com/article/10.1007/s00299-021-02827-w https://doi.org/10.1007/s00299-021-02827-w |
| _version_ | 1855484811556159488 |
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| author | Bottero, Ana Emilia Gomez, Maria Cristina Stritzler, Margarita Tajima, Hiromi Frare, Romina Alejandra Pascuan, Cecilia Gabriela Blumwald, Eduardo Ayub, Nicolás Daniel Soto, Gabriela Cynthia |
| author_browse | Ayub, Nicolás Daniel Blumwald, Eduardo Bottero, Ana Emilia Frare, Romina Alejandra Gomez, Maria Cristina Pascuan, Cecilia Gabriela Soto, Gabriela Cynthia Stritzler, Margarita Tajima, Hiromi |
| author_facet | Bottero, Ana Emilia Gomez, Maria Cristina Stritzler, Margarita Tajima, Hiromi Frare, Romina Alejandra Pascuan, Cecilia Gabriela Blumwald, Eduardo Ayub, Nicolás Daniel Soto, Gabriela Cynthia |
| author_sort | Bottero, Ana Emilia |
| collection | INTA Digital |
| description | We present the first report on base editing in alfalfa. Specifically, we showed edited alfalfa with tolerance to both sulfonylurea- and imidazolinone-type herbicides. Alfalfa (Medicago sativa) is one of the most important forage legumes worldwide. However, alfalfa-breeding programs are limited by the particular reproductive features of the species itself. Cultivated alfalfa is an allogamous perennial polyploid species that exhibits high levels of self-incompatibility and strong inbreeding depression (Dieterich Mabin et al. 2021). Consequently, the rapid and inexpensive introgression of both transgenes and edited alleles into elite alfalfa germplasm requires the use of dominant traits (Bottero et al. 2021; Jozefkowicz et al. 2018). In this context, the commercial application of full knockout of recessive genes in alfalfa efficiently generated via the CRISPR/Cas9 system (Bottero et al. 2021; Chen et al. 2020; Wolabu et al. 2020). |
| format | Artículo |
| id | INTA11592 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA115922022-04-08T10:34:43Z Generation of a multi‑herbicide‑tolerant alfalfa by using base editing Bottero, Ana Emilia Gomez, Maria Cristina Stritzler, Margarita Tajima, Hiromi Frare, Romina Alejandra Pascuan, Cecilia Gabriela Blumwald, Eduardo Ayub, Nicolás Daniel Soto, Gabriela Cynthia Medicago sativa Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Herbicidas CRISPR Herbicides Base Editing Lucerne Edición Base Alfalfa We present the first report on base editing in alfalfa. Specifically, we showed edited alfalfa with tolerance to both sulfonylurea- and imidazolinone-type herbicides. Alfalfa (Medicago sativa) is one of the most important forage legumes worldwide. However, alfalfa-breeding programs are limited by the particular reproductive features of the species itself. Cultivated alfalfa is an allogamous perennial polyploid species that exhibits high levels of self-incompatibility and strong inbreeding depression (Dieterich Mabin et al. 2021). Consequently, the rapid and inexpensive introgression of both transgenes and edited alleles into elite alfalfa germplasm requires the use of dominant traits (Bottero et al. 2021; Jozefkowicz et al. 2018). In this context, the commercial application of full knockout of recessive genes in alfalfa efficiently generated via the CRISPR/Cas9 system (Bottero et al. 2021; Chen et al. 2020; Wolabu et al. 2020). Instituto de Biotecnología Fil: Bottero, Ana Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Bottero, Ana Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Bottero, Ana Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Gomez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Stritzler, Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tajima, Hiromi. University of California. Department of Plant Sciences; Estados Unidos Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Frare, Romina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Pascuan, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Blumwald, Eduardo. University of California. Department of Plant Sciences; Estados Unidos Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2022-04-08T10:29:28Z 2022-04-08T10:29:28Z 2022-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11592 https://link.springer.com/article/10.1007/s00299-021-02827-w 1432-203X https://doi.org/10.1007/s00299-021-02827-w eng info:eu-repograntAgreement/INTA/2019-PE-E6-I115-001/2019-PE-E6-I115-001/AR./Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuario info:eu-repo/semantics/restrictedAccess application/pdf Springer Plant Cell Reports 41 (2) : 493-495 (Febrero 2022) |
| spellingShingle | Medicago sativa Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Herbicidas CRISPR Herbicides Base Editing Lucerne Edición Base Alfalfa Bottero, Ana Emilia Gomez, Maria Cristina Stritzler, Margarita Tajima, Hiromi Frare, Romina Alejandra Pascuan, Cecilia Gabriela Blumwald, Eduardo Ayub, Nicolás Daniel Soto, Gabriela Cynthia Generation of a multi‑herbicide‑tolerant alfalfa by using base editing |
| title | Generation of a multi‑herbicide‑tolerant alfalfa by using base editing |
| title_full | Generation of a multi‑herbicide‑tolerant alfalfa by using base editing |
| title_fullStr | Generation of a multi‑herbicide‑tolerant alfalfa by using base editing |
| title_full_unstemmed | Generation of a multi‑herbicide‑tolerant alfalfa by using base editing |
| title_short | Generation of a multi‑herbicide‑tolerant alfalfa by using base editing |
| title_sort | generation of a multi herbicide tolerant alfalfa by using base editing |
| topic | Medicago sativa Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Herbicidas CRISPR Herbicides Base Editing Lucerne Edición Base Alfalfa |
| url | http://hdl.handle.net/20.500.12123/11592 https://link.springer.com/article/10.1007/s00299-021-02827-w https://doi.org/10.1007/s00299-021-02827-w |
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