Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle
Vaccines against bovine babesiosis must, ideally, induce a humoral immune response characterized by neutralizing antibodies against conserved epitopes and a cellular Th1 immune response. In Babesia bovis, proteins such as AMA-1, MSA-2c, and RAP-1 have been characterized and antibodies against these...
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2022
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/11574 https://www.sciencedirect.com/science/article/pii/S0264410X22000494 https://doi.org/10.1016/j.vaccine.2022.01.023 |
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| author | Hidalgo-Ruiz, Mario Mejia-López, Susana Pérez-Serrano, Rosa M. Zaldívar-Lelo de Larrea, Guadalupe Ganzinelli, Sabrina Belen Florin-Christensen, Monica Suarez, Carlos Esteban Hernández-Ortiz, Rubén Mercado-Uriostegui, Miguel A. Rodríguez-Torres, Angelina Carvajal-Gamez, Bertha I. Camacho-Nuez, Minerva Wilkowsky, Silvina Elizabeth Mosqueda, Juan |
| author_browse | Camacho-Nuez, Minerva Carvajal-Gamez, Bertha I. Florin-Christensen, Monica Ganzinelli, Sabrina Belen Hernández-Ortiz, Rubén Hidalgo-Ruiz, Mario Mejia-López, Susana Mercado-Uriostegui, Miguel A. Mosqueda, Juan Pérez-Serrano, Rosa M. Rodríguez-Torres, Angelina Suarez, Carlos Esteban Wilkowsky, Silvina Elizabeth Zaldívar-Lelo de Larrea, Guadalupe |
| author_facet | Hidalgo-Ruiz, Mario Mejia-López, Susana Pérez-Serrano, Rosa M. Zaldívar-Lelo de Larrea, Guadalupe Ganzinelli, Sabrina Belen Florin-Christensen, Monica Suarez, Carlos Esteban Hernández-Ortiz, Rubén Mercado-Uriostegui, Miguel A. Rodríguez-Torres, Angelina Carvajal-Gamez, Bertha I. Camacho-Nuez, Minerva Wilkowsky, Silvina Elizabeth Mosqueda, Juan |
| author_sort | Hidalgo-Ruiz, Mario |
| collection | INTA Digital |
| description | Vaccines against bovine babesiosis must, ideally, induce a humoral immune response characterized by neutralizing antibodies against conserved epitopes and a cellular Th1 immune response. In Babesia bovis, proteins such as AMA-1, MSA-2c, and RAP-1 have been characterized and antibodies against these proteins have shown a neutralizing effect, demonstrating the implication of B and T-cell epitopes in the immune response. There is evidence of the existence of B and T-cell epitopes in these proteins, however, it remains to be defined, the presence of conserved peptides in strains from around the world containing B and T-cell epitopes, and their role in the generation of a long-lasting immunity. The aim in this paper was to identify peptides of Babesia bovis AMA-1, MSA-2c, and RAP-1 that elicit a neutralizing and long-lasting Th1 immune response. Peptides containing B-cell epitopes of AMA-1, MSA-2c and RAP-1, were identified. The immune response generated by each peptide was characterized in cattle. All peptides tested induced antibodies that recognized intraerythrocytic parasites, however, only 5 peptides generated neutralizing antibodies in vitro: P2AMA-1 (6.28%), P3MSA-2c (10.27%), P4MSA-2c (10.42%), P1RAP-1 (32.45%), and P4RAP-1 (36.98%). When these neutralizing antibodies were evaluated as a pool, the inhibition percentage of invasion increased to 52.37%. When the T cellular response was evaluated, two peptides: P3MSA2c and P2AMA1 induced a higher percentage (>70%) of activated CD4 +/CD45RO+ T cells than unstimulated cells. Additionally, both peptides induced the production of gamma interferon (IFN-) in PBMCs from vaccinated cattle after one year proving the implication of a long-lasting Th1 immune response. In conclusion, we identified conserved peptides containing B and T-cell epitopes in antigens of B. bovis that elicit a Th1 immune response and showed evidence that peptides from the same protein elicit different immune responses, which has implication for vaccine development in bovine babesiosis. |
| format | Artículo |
| id | INTA11574 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA115742022-04-07T10:52:41Z Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle Hidalgo-Ruiz, Mario Mejia-López, Susana Pérez-Serrano, Rosa M. Zaldívar-Lelo de Larrea, Guadalupe Ganzinelli, Sabrina Belen Florin-Christensen, Monica Suarez, Carlos Esteban Hernández-Ortiz, Rubén Mercado-Uriostegui, Miguel A. Rodríguez-Torres, Angelina Carvajal-Gamez, Bertha I. Camacho-Nuez, Minerva Wilkowsky, Silvina Elizabeth Mosqueda, Juan Babesia bovis Linfocitos-t Interferonas Anticuerpos Respuesta Inmunológica Neutralización T-lymphocytes Interferons Antibodies Immune Response Neutralization T-cell Células-t Vaccines against bovine babesiosis must, ideally, induce a humoral immune response characterized by neutralizing antibodies against conserved epitopes and a cellular Th1 immune response. In Babesia bovis, proteins such as AMA-1, MSA-2c, and RAP-1 have been characterized and antibodies against these proteins have shown a neutralizing effect, demonstrating the implication of B and T-cell epitopes in the immune response. There is evidence of the existence of B and T-cell epitopes in these proteins, however, it remains to be defined, the presence of conserved peptides in strains from around the world containing B and T-cell epitopes, and their role in the generation of a long-lasting immunity. The aim in this paper was to identify peptides of Babesia bovis AMA-1, MSA-2c, and RAP-1 that elicit a neutralizing and long-lasting Th1 immune response. Peptides containing B-cell epitopes of AMA-1, MSA-2c and RAP-1, were identified. The immune response generated by each peptide was characterized in cattle. All peptides tested induced antibodies that recognized intraerythrocytic parasites, however, only 5 peptides generated neutralizing antibodies in vitro: P2AMA-1 (6.28%), P3MSA-2c (10.27%), P4MSA-2c (10.42%), P1RAP-1 (32.45%), and P4RAP-1 (36.98%). When these neutralizing antibodies were evaluated as a pool, the inhibition percentage of invasion increased to 52.37%. When the T cellular response was evaluated, two peptides: P3MSA2c and P2AMA1 induced a higher percentage (>70%) of activated CD4 +/CD45RO+ T cells than unstimulated cells. Additionally, both peptides induced the production of gamma interferon (IFN-) in PBMCs from vaccinated cattle after one year proving the implication of a long-lasting Th1 immune response. In conclusion, we identified conserved peptides containing B and T-cell epitopes in antigens of B. bovis that elicit a Th1 immune response and showed evidence that peptides from the same protein elicit different immune responses, which has implication for vaccine development in bovine babesiosis. Instituto de Patobiología Fil: Hidalgo-Ruiz, Mario. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México Fil: Mejia-López, Susana. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México Fil: Pérez-Serrano, Rosa M. Universidad Autónoma de Querétaro. Facultad de Medicina; México Fil: Zaldívar-Lelo de Larrea, Guadalupe. Universidad Autónoma de Querétaro. Facultad de Medicina; México Fil: Ganzinelli Sabrina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria (IPVET); Argentina Fil: Ganzinelli Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria (IPVET); Argentina Fil: Florin-Christensen, Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Suarez, Carlos Esteban. United States Department of Agricultural-Agricultural Research Service. Animal Disease Research Unit; Estados Unidos Fil: Hernández-Ortiz, Rubén. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP). Centro de Estudios e Investigaciones para el Desarrollo Docente (CENID). Salud Animal e Inocuidad; México Fil: Mercado-Uriostegui, Miguel A. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México Fil: Rodríguez-Torres, Angelina. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México Fil: Carvajal-Gamez, Bertha I. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México Fil: Camacho-Nuez, Minerva. Universidad Autónoma de la Ciudad de México. Posgrado en Ciencias Genómicas; México Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Mosqueda, Juan. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México 2022-04-07T10:43:44Z 2022-04-07T10:43:44Z 2022-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11574 https://www.sciencedirect.com/science/article/pii/S0264410X22000494 0264-410X https://doi.org/10.1016/j.vaccine.2022.01.023 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Vaccine 40 (8) : 1108-1115 (Febrero 2022) |
| spellingShingle | Babesia bovis Linfocitos-t Interferonas Anticuerpos Respuesta Inmunológica Neutralización T-lymphocytes Interferons Antibodies Immune Response Neutralization T-cell Células-t Hidalgo-Ruiz, Mario Mejia-López, Susana Pérez-Serrano, Rosa M. Zaldívar-Lelo de Larrea, Guadalupe Ganzinelli, Sabrina Belen Florin-Christensen, Monica Suarez, Carlos Esteban Hernández-Ortiz, Rubén Mercado-Uriostegui, Miguel A. Rodríguez-Torres, Angelina Carvajal-Gamez, Bertha I. Camacho-Nuez, Minerva Wilkowsky, Silvina Elizabeth Mosqueda, Juan Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle |
| title | Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle |
| title_full | Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle |
| title_fullStr | Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle |
| title_full_unstemmed | Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle |
| title_short | Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle |
| title_sort | babesia bovis ama 1 msa 2c and rap 1 contain conserved b and t cell epitopes which generate neutralizing antibodies and a long lasting th1 immune response in vaccinated cattle |
| topic | Babesia bovis Linfocitos-t Interferonas Anticuerpos Respuesta Inmunológica Neutralización T-lymphocytes Interferons Antibodies Immune Response Neutralization T-cell Células-t |
| url | http://hdl.handle.net/20.500.12123/11574 https://www.sciencedirect.com/science/article/pii/S0264410X22000494 https://doi.org/10.1016/j.vaccine.2022.01.023 |
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