Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia
We have previously shown the extensive loss of genes during the domestication of alfalfa rhizobia and the high nitrous oxide emission associated with the extreme genomic instability of commercial inoculants. In the present note, we describe the molecular mechanism involved in the evolution of alfalf...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/11573 https://link.springer.com/article/10.1007/s00248-021-01761-4 https://doi.org/10.1007/s00248-021-01761-4 |
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| author | Frare, Romina Alejandra Pascuan, Cecilia Gabriela Galindo Sotomonte, Luisa Fernanda McCormick, Wayne Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
| author_browse | Ayub, Nicolás Daniel Frare, Romina Alejandra Galindo Sotomonte, Luisa Fernanda McCormick, Wayne Pascuan, Cecilia Gabriela Soto, Gabriela Cynthia |
| author_facet | Frare, Romina Alejandra Pascuan, Cecilia Gabriela Galindo Sotomonte, Luisa Fernanda McCormick, Wayne Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
| author_sort | Frare, Romina Alejandra |
| collection | INTA Digital |
| description | We have previously shown the extensive loss of genes during the domestication of alfalfa rhizobia and the high nitrous oxide emission associated with the extreme genomic instability of commercial inoculants. In the present note, we describe the molecular mechanism involved in the evolution of alfalfa rhizobia. Genomic analysis showed that most of the gene losses in inoculants are due to large genomic deletions rather than to small deletions or point mutations, a fact consistent with recurrent DNA double-strand breaks (DSBs) at numerous locations throughout the microbial genome. Genetic analysis showed that the loss of the NO-detoxifying enzyme HmpA in inoculants results in growth inhibition and high DSB levels under nitrosative stress, and large genomic deletions in planta but not in the soil. Therefore, besides its known function in the effective establishment of the symbiosis, HmpA can play a critical role in the preservation of the genomic integrity of alfalfa rhizobia under host-derived nitrosative stress. |
| format | Artículo |
| id | INTA11573 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA115732022-04-06T15:39:03Z Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia Frare, Romina Alejandra Pascuan, Cecilia Gabriela Galindo Sotomonte, Luisa Fernanda McCormick, Wayne Soto, Gabriela Cynthia Ayub, Nicolás Daniel DNA Damage Domestication Nitric Oxide Daño al ADN Domesticación Óxido Nítrico Medicago sativa Rhizobiaceae Evolutionary Mechanism Lucerne Mecanismo Evolutivo Alfalfa We have previously shown the extensive loss of genes during the domestication of alfalfa rhizobia and the high nitrous oxide emission associated with the extreme genomic instability of commercial inoculants. In the present note, we describe the molecular mechanism involved in the evolution of alfalfa rhizobia. Genomic analysis showed that most of the gene losses in inoculants are due to large genomic deletions rather than to small deletions or point mutations, a fact consistent with recurrent DNA double-strand breaks (DSBs) at numerous locations throughout the microbial genome. Genetic analysis showed that the loss of the NO-detoxifying enzyme HmpA in inoculants results in growth inhibition and high DSB levels under nitrosative stress, and large genomic deletions in planta but not in the soil. Therefore, besides its known function in the effective establishment of the symbiosis, HmpA can play a critical role in the preservation of the genomic integrity of alfalfa rhizobia under host-derived nitrosative stress. Instituto de Biotecnología Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Frare, Romina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Pascuan, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Galindo Sotomonte, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Galindo Sotomonte, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Galindo Sotomonte, Luisa Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: McCormick, Wayne. Ottawa Research and Development Centre; Canadá Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Soto, Gabriela Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2022-04-06T15:32:27Z 2022-04-06T15:32:27Z 2022-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11573 https://link.springer.com/article/10.1007/s00248-021-01761-4 1432-184X https://doi.org/10.1007/s00248-021-01761-4 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Microbial Ecology 83 (2) : 501-505 (Febrero 2022) |
| spellingShingle | DNA Damage Domestication Nitric Oxide Daño al ADN Domesticación Óxido Nítrico Medicago sativa Rhizobiaceae Evolutionary Mechanism Lucerne Mecanismo Evolutivo Alfalfa Frare, Romina Alejandra Pascuan, Cecilia Gabriela Galindo Sotomonte, Luisa Fernanda McCormick, Wayne Soto, Gabriela Cynthia Ayub, Nicolás Daniel Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia |
| title | Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia |
| title_full | Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia |
| title_fullStr | Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia |
| title_full_unstemmed | Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia |
| title_short | Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia |
| title_sort | exploring the role of the no detoxifying enzyme hmpa in the evolution of domesticated alfalfa rhizobia |
| topic | DNA Damage Domestication Nitric Oxide Daño al ADN Domesticación Óxido Nítrico Medicago sativa Rhizobiaceae Evolutionary Mechanism Lucerne Mecanismo Evolutivo Alfalfa |
| url | http://hdl.handle.net/20.500.12123/11573 https://link.springer.com/article/10.1007/s00248-021-01761-4 https://doi.org/10.1007/s00248-021-01761-4 |
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