The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene
The barley chloroplast mutator (cpm) is a nuclear gene mutant that induces a wide spectrum of cytoplasmically inherited chlorophyll deficiencies. Plastome instability of cpm seedlings was determined by identification of a particular landscape of polymorphisms that suggests failures in a plastome mis...
| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/11564 https://www.mdpi.com/1422-0067/23/3/1814 https://doi.org/10.3390/ijms23031814 |
| _version_ | 1855484806241976320 |
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| author | Lencina, Franco Daniel Landau, Alejandra Mabel Prina, Alberto Raul |
| author_browse | Landau, Alejandra Mabel Lencina, Franco Daniel Prina, Alberto Raul |
| author_facet | Lencina, Franco Daniel Landau, Alejandra Mabel Prina, Alberto Raul |
| author_sort | Lencina, Franco Daniel |
| collection | INTA Digital |
| description | The barley chloroplast mutator (cpm) is a nuclear gene mutant that induces a wide spectrum of cytoplasmically inherited chlorophyll deficiencies. Plastome instability of cpm seedlings was determined by identification of a particular landscape of polymorphisms that suggests failures in a plastome mismatch repair (MMR) protein. In Arabidopsis, MSH genes encode proteins that are in charge of mismatch repair and have anti-recombination activity. In this work, barley homologs of these genes were identified, and their sequences were analyzed in control and cpm mutant seedlings. A substitution, leading to a premature stop codon and a truncated MSH1 protein, was identified in the Msh1 gene of cpm plants. The relationship between this mutation and the presence of chlorophyll deficiencies was established in progenies from crosses and backcrosses. These results strongly suggest that the mutation identified in the Msh1 gene of the cpm mutant is responsible for the observed plastome instabilities. Interestingly, comparison of mutant phenotypes and molecular changes induced by the barley cpm mutant with those of Arabidopsis MSH1 mutants revealed marked differences. |
| format | Artículo |
| id | INTA11564 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA115642022-04-06T10:18:36Z The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene Lencina, Franco Daniel Landau, Alejandra Mabel Prina, Alberto Raul Barley Chloroplasts Mutants Cebada Cloroplasto Mutantes Barley Chloroplast Mutator Msh1 gene Plastome Mutante del Cloroplasto de Cebada Gen Msh1 Plastoma The barley chloroplast mutator (cpm) is a nuclear gene mutant that induces a wide spectrum of cytoplasmically inherited chlorophyll deficiencies. Plastome instability of cpm seedlings was determined by identification of a particular landscape of polymorphisms that suggests failures in a plastome mismatch repair (MMR) protein. In Arabidopsis, MSH genes encode proteins that are in charge of mismatch repair and have anti-recombination activity. In this work, barley homologs of these genes were identified, and their sequences were analyzed in control and cpm mutant seedlings. A substitution, leading to a premature stop codon and a truncated MSH1 protein, was identified in the Msh1 gene of cpm plants. The relationship between this mutation and the presence of chlorophyll deficiencies was established in progenies from crosses and backcrosses. These results strongly suggest that the mutation identified in the Msh1 gene of the cpm mutant is responsible for the observed plastome instabilities. Interestingly, comparison of mutant phenotypes and molecular changes induced by the barley cpm mutant with those of Arabidopsis MSH1 mutants revealed marked differences. Instituto de Genética Fil: Lencina, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Landau, Alejandra Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Prina, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2022-04-06T10:08:43Z 2022-04-06T10:08:43Z 2022-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11564 https://www.mdpi.com/1422-0067/23/3/1814 1422-0067 https://doi.org/10.3390/ijms23031814 eng info:eu-repograntAgreement/INTA/PNBIO-1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos. info:eu-repograntAgreement/INTA/2019-PE-E6-I115-001/2019-PE-E6-I115-001/AR./Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuario info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI International Journal of Molecular Sciences 23 (3) : 1814 (Febrero 2022) |
| spellingShingle | Barley Chloroplasts Mutants Cebada Cloroplasto Mutantes Barley Chloroplast Mutator Msh1 gene Plastome Mutante del Cloroplasto de Cebada Gen Msh1 Plastoma Lencina, Franco Daniel Landau, Alejandra Mabel Prina, Alberto Raul The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene |
| title | The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene |
| title_full | The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene |
| title_fullStr | The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene |
| title_full_unstemmed | The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene |
| title_short | The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene |
| title_sort | barley chloroplast mutator cpm mutant all roads lead to the msh1 gene |
| topic | Barley Chloroplasts Mutants Cebada Cloroplasto Mutantes Barley Chloroplast Mutator Msh1 gene Plastome Mutante del Cloroplasto de Cebada Gen Msh1 Plastoma |
| url | http://hdl.handle.net/20.500.12123/11564 https://www.mdpi.com/1422-0067/23/3/1814 https://doi.org/10.3390/ijms23031814 |
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