Analysis of the gut bacterial community of wild larvae of Anastrepha fraterculus sp. 1: effect of host fruit, environment, and prominent stable associations of the genera Wolbachia, Tatumella, and Enterobacter
The genus Anastrepha (Diptera Tephritidae) includes some of the most important fruit fly pests in the Americas. Here, we studied the gut bacterial community of 3rd instar larvae of Anastrepha fraterculus sp. 1 through Next Generation Sequencing (lllumina) of the V3-V4 hypervariable region within the...
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| Format: | Artículo |
| Language: | Inglés |
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Frontiers Media
2022
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/11550 https://www.frontiersin.org/articles/10.3389/fmicb.2022.822990/full https://doi.org/10.3389/fmicb.2022.822990 |
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| author | Salgueiro, Julieta Nussenbaum, Ana Laura Milla, Fabian Horacio Asimakis, Elias D. Goane, Lucía Ruiz, María Josefina Bachmann, Guillermo Enrique Vera, María Teresa Stathopoulou, Panagiota Bourtzis, Kostas Deutscher, Ania T. Lanzavecchia, Silvia Beatriz Tsiamis, George Segura, Diego Fernando |
| author_browse | Asimakis, Elias D. Bachmann, Guillermo Enrique Bourtzis, Kostas Deutscher, Ania T. Goane, Lucía Lanzavecchia, Silvia Beatriz Milla, Fabian Horacio Nussenbaum, Ana Laura Ruiz, María Josefina Salgueiro, Julieta Segura, Diego Fernando Stathopoulou, Panagiota Tsiamis, George Vera, María Teresa |
| author_facet | Salgueiro, Julieta Nussenbaum, Ana Laura Milla, Fabian Horacio Asimakis, Elias D. Goane, Lucía Ruiz, María Josefina Bachmann, Guillermo Enrique Vera, María Teresa Stathopoulou, Panagiota Bourtzis, Kostas Deutscher, Ania T. Lanzavecchia, Silvia Beatriz Tsiamis, George Segura, Diego Fernando |
| author_sort | Salgueiro, Julieta |
| collection | INTA Digital |
| description | The genus Anastrepha (Diptera Tephritidae) includes some of the most important fruit fly pests in the Americas. Here, we studied the gut bacterial community of 3rd instar larvae of Anastrepha fraterculus sp. 1 through Next Generation Sequencing (lllumina) of the V3-V4 hypervariable region within the 16S rRNA gene. Gut bacterial communities were compared between host species (guava and peach), and geographical origins (Concordia and Horco Molle in Argentina) representing distinct ecological scenarios. In addition, we explored the effect of spatial scale by comparing the samples collected from different trees within each geographic origin and host species. We also addressed the effect of fruit size on bacterial diversity. The gut bacterial community was affected both by host species and geographic origin. At smaller spatial scales, the gut bacterial profile differed among trees of the same species and location at least in one host-location combination. There was no effect of fruit size on the larval gut bacteriome. Operational Taxonomic Units (OTUs) assigned to Wolbachia, Tatumella and Enterobacter were identified in all samples examined, which suggest potential, non transient symbioses. Better knowledge on the larval gut bacteriome contributes valuable information to develop sustainable control strategies against A. fraterculus targeting key symbionts as the Achilles’ heel to control this important fruit fly pest. |
| format | Artículo |
| id | INTA11550 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA115502022-04-06T10:04:05Z Analysis of the gut bacterial community of wild larvae of Anastrepha fraterculus sp. 1: effect of host fruit, environment, and prominent stable associations of the genera Wolbachia, Tatumella, and Enterobacter Salgueiro, Julieta Nussenbaum, Ana Laura Milla, Fabian Horacio Asimakis, Elias D. Goane, Lucía Ruiz, María Josefina Bachmann, Guillermo Enrique Vera, María Teresa Stathopoulou, Panagiota Bourtzis, Kostas Deutscher, Ania T. Lanzavecchia, Silvia Beatriz Tsiamis, George Segura, Diego Fernando Tephritidae Anastrepha fraterculus Wolbachia Enterobacter Liberación de Insectos Estériles Sterile Insect Release Sterile Insect Technique Tatumella Técnica del Insecto Estéril The genus Anastrepha (Diptera Tephritidae) includes some of the most important fruit fly pests in the Americas. Here, we studied the gut bacterial community of 3rd instar larvae of Anastrepha fraterculus sp. 1 through Next Generation Sequencing (lllumina) of the V3-V4 hypervariable region within the 16S rRNA gene. Gut bacterial communities were compared between host species (guava and peach), and geographical origins (Concordia and Horco Molle in Argentina) representing distinct ecological scenarios. In addition, we explored the effect of spatial scale by comparing the samples collected from different trees within each geographic origin and host species. We also addressed the effect of fruit size on bacterial diversity. The gut bacterial community was affected both by host species and geographic origin. At smaller spatial scales, the gut bacterial profile differed among trees of the same species and location at least in one host-location combination. There was no effect of fruit size on the larval gut bacteriome. Operational Taxonomic Units (OTUs) assigned to Wolbachia, Tatumella and Enterobacter were identified in all samples examined, which suggest potential, non transient symbioses. Better knowledge on the larval gut bacteriome contributes valuable information to develop sustainable control strategies against A. fraterculus targeting key symbionts as the Achilles’ heel to control this important fruit fly pest. Instituto de Genética Fil: Salgueiro, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina Fil: Salgueiro, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Salgueiro, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Nussenbaum, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Milla, Fabian Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina Fil: Milla, Fabian Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Asimakis, Elias D. University of Patras. Department of Environmental Engineering; Grecia Fil: Goane, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Goane, Lucía. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina Fil: Ruiz, María Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ruiz, María Josefina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina Fil: Bachmann, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina Fil: Bachmann, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Bachmann, Guillermo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vera, María Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vera, María Teresa. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina Fil: Stathopoulou, Panagiota. University of Patras. Department of Environmental Engineering; Grecia Fil: Bourtzis, Kostas. Vienna International Centre. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture. Insect Pest Control Laboratory; Austria Fil: Deutscher, Ania T. Biosecurity and Food Safety. Department of Primary Industries, Elizabeth Macarthur Agricultural Institute (EMAI); Australia Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Tsiamis, George. University of Patras. Department of Environmental Engineering; Grecia Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Segura, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2022-04-04T17:27:15Z 2022-04-04T17:27:15Z 2022-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11550 https://www.frontiersin.org/articles/10.3389/fmicb.2022.822990/full 1664-302X https://doi.org/10.3389/fmicb.2022.822990 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I079-001/2019-PD-E4-I079-001/AR./Genética, genómica y ecología de insectos de importancia agronómica como insumo para el desarrollo de estrategias sustentables de control plagas info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Microbiology 13 : 822990 (Marzo 2022) |
| spellingShingle | Tephritidae Anastrepha fraterculus Wolbachia Enterobacter Liberación de Insectos Estériles Sterile Insect Release Sterile Insect Technique Tatumella Técnica del Insecto Estéril Salgueiro, Julieta Nussenbaum, Ana Laura Milla, Fabian Horacio Asimakis, Elias D. Goane, Lucía Ruiz, María Josefina Bachmann, Guillermo Enrique Vera, María Teresa Stathopoulou, Panagiota Bourtzis, Kostas Deutscher, Ania T. Lanzavecchia, Silvia Beatriz Tsiamis, George Segura, Diego Fernando Analysis of the gut bacterial community of wild larvae of Anastrepha fraterculus sp. 1: effect of host fruit, environment, and prominent stable associations of the genera Wolbachia, Tatumella, and Enterobacter |
| title | Analysis of the gut bacterial community of wild larvae of Anastrepha fraterculus sp. 1: effect of host fruit, environment, and prominent stable associations of the genera Wolbachia, Tatumella, and Enterobacter |
| title_full | Analysis of the gut bacterial community of wild larvae of Anastrepha fraterculus sp. 1: effect of host fruit, environment, and prominent stable associations of the genera Wolbachia, Tatumella, and Enterobacter |
| title_fullStr | Analysis of the gut bacterial community of wild larvae of Anastrepha fraterculus sp. 1: effect of host fruit, environment, and prominent stable associations of the genera Wolbachia, Tatumella, and Enterobacter |
| title_full_unstemmed | Analysis of the gut bacterial community of wild larvae of Anastrepha fraterculus sp. 1: effect of host fruit, environment, and prominent stable associations of the genera Wolbachia, Tatumella, and Enterobacter |
| title_short | Analysis of the gut bacterial community of wild larvae of Anastrepha fraterculus sp. 1: effect of host fruit, environment, and prominent stable associations of the genera Wolbachia, Tatumella, and Enterobacter |
| title_sort | analysis of the gut bacterial community of wild larvae of anastrepha fraterculus sp 1 effect of host fruit environment and prominent stable associations of the genera wolbachia tatumella and enterobacter |
| topic | Tephritidae Anastrepha fraterculus Wolbachia Enterobacter Liberación de Insectos Estériles Sterile Insect Release Sterile Insect Technique Tatumella Técnica del Insecto Estéril |
| url | http://hdl.handle.net/20.500.12123/11550 https://www.frontiersin.org/articles/10.3389/fmicb.2022.822990/full https://doi.org/10.3389/fmicb.2022.822990 |
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