Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR
Alfalfa is the most important forage legume worldwide. However, similar to other minor forage crops, it is usually harvested along with weeds, which decrease its nutrient quality and thus reduce its high value in the market. In addition, weeds reduce alfalfa yield by about 50 %. Although weeds are...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1152 https://link.springer.com/article/10.1007%2Fs00299-016-2026-7 https://doi.org/10.1007/s00299-016-2026-7 |
| _version_ | 1855034756294508544 |
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| author | Bottero, Ana Emilia Pascuan, Cecilia Gabriela Pagano, Elba Maria Ayub, Nicolás Daniel Soto, Gabriela Cinthia Jozefkowicz, Cintia |
| author_browse | Ayub, Nicolás Daniel Bottero, Ana Emilia Jozefkowicz, Cintia Pagano, Elba Maria Pascuan, Cecilia Gabriela Soto, Gabriela Cinthia |
| author_facet | Bottero, Ana Emilia Pascuan, Cecilia Gabriela Pagano, Elba Maria Ayub, Nicolás Daniel Soto, Gabriela Cinthia Jozefkowicz, Cintia |
| author_sort | Bottero, Ana Emilia |
| collection | INTA Digital |
| description | Alfalfa is the most important forage legume worldwide. However, similar to other minor forage crops, it is usually harvested along with weeds, which decrease its nutrient quality and thus reduce its high value in the market.
In addition, weeds reduce alfalfa yield by about 50 %.
Although weeds are the limiting factor for alfalfa production, little progress has been made in the incorporation of herbicide-tolerant traits into commercial alfalfa. This is partially due to the high times and costs needed for the
production of vast numbers of transgenic alfalfa events as an empirical approach to bypass the random transgenic silencing and for the identification of an event with optimal transgene expression. In this focus article, we report the
complete sequence of pPZP200BAR and the extremely high efficiency of this binary vector in alfalfa transformation, opening the way for rapid and inexpensive production of transgenic events for alfalfa improvement public
programs. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1152 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA11522019-03-22T13:39:44Z Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR Bottero, Ana Emilia Pascuan, Cecilia Gabriela Pagano, Elba Maria Ayub, Nicolás Daniel Soto, Gabriela Cinthia Jozefkowicz, Cintia Genética Medicago Sativa Vectores Plantas Transgénicas Genetics Vectors Transgenic Plants Alfalfa Alfalfa is the most important forage legume worldwide. However, similar to other minor forage crops, it is usually harvested along with weeds, which decrease its nutrient quality and thus reduce its high value in the market. In addition, weeds reduce alfalfa yield by about 50 %. Although weeds are the limiting factor for alfalfa production, little progress has been made in the incorporation of herbicide-tolerant traits into commercial alfalfa. This is partially due to the high times and costs needed for the production of vast numbers of transgenic alfalfa events as an empirical approach to bypass the random transgenic silencing and for the identification of an event with optimal transgene expression. In this focus article, we report the complete sequence of pPZP200BAR and the extremely high efficiency of this binary vector in alfalfa transformation, opening the way for rapid and inexpensive production of transgenic events for alfalfa improvement public programs. Inst. de Genética "Ewald A. Favret"- IGEAF Fil: Bottero, Ana Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-09-06T15:07:09Z 2017-09-06T15:07:09Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1152 https://link.springer.com/article/10.1007%2Fs00299-016-2026-7 0721-7714 (Print) 1432-203X (Online) https://doi.org/10.1007/s00299-016-2026-7 eng info:eu-repo/semantics/restrictedAccess application/pdf Plant cell reports 35 (9) : 1987–1990. (September 2016) |
| spellingShingle | Genética Medicago Sativa Vectores Plantas Transgénicas Genetics Vectors Transgenic Plants Alfalfa Bottero, Ana Emilia Pascuan, Cecilia Gabriela Pagano, Elba Maria Ayub, Nicolás Daniel Soto, Gabriela Cinthia Jozefkowicz, Cintia Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR |
| title | Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR |
| title_full | Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR |
| title_fullStr | Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR |
| title_full_unstemmed | Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR |
| title_short | Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR |
| title_sort | minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector ppzp200bar |
| topic | Genética Medicago Sativa Vectores Plantas Transgénicas Genetics Vectors Transgenic Plants Alfalfa |
| url | http://hdl.handle.net/20.500.12123/1152 https://link.springer.com/article/10.1007%2Fs00299-016-2026-7 https://doi.org/10.1007/s00299-016-2026-7 |
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