Evaluation of different heterologous prime-boost immunization strategies against Babesia bovis using viral vectored and protein-adjuvant vaccines based on a chimeric multi-antigen
Protection against the intraerythrocytic bovine parasite Babesia bovis requires both humoral and cellular immune responses. Therefore, tailored combinations of immunogens targeted at both arms of the immune system are strategies of choice to pursue sterilizing immunity. In this study, different hete...
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1150 http://www.sciencedirect.com/science/article/pii/S0264410X16303796?via%3Dihub https://link.springer.com/article/10.1007%2Fs00299-016-2026-7 https://doi.org/10.1016/j.vaccine.2016.05.053 |
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| author | Jaramillo Ortiz, Jose Manuel Molinari, Maria Paula Gravisaco, María José Paoletta, Martina Montenegro, Valeria Noely Wilkowsky, Silvina Elizabeth |
| author_browse | Gravisaco, María José Jaramillo Ortiz, Jose Manuel Molinari, Maria Paula Montenegro, Valeria Noely Paoletta, Martina Wilkowsky, Silvina Elizabeth |
| author_facet | Jaramillo Ortiz, Jose Manuel Molinari, Maria Paula Gravisaco, María José Paoletta, Martina Montenegro, Valeria Noely Wilkowsky, Silvina Elizabeth |
| author_sort | Jaramillo Ortiz, Jose Manuel |
| collection | INTA Digital |
| description | Protection against the intraerythrocytic bovine parasite Babesia bovis requires both humoral and cellular immune responses. Therefore, tailored combinations of immunogens targeted at both arms of the immune system are strategies of choice to pursue sterilizing immunity. In this study, different heterologous prime-boost vaccination schemes were evaluated in mice to compare the immunogenicity induced by a recombinant adenovirus, a modified vaccinia Ankara vector or a subunit vaccine all expressing a chimeric multi-antigen. This multi-antigen includes the immunodominant B and T cell epitopes of three B. bovis proteins: Merozoite Surface Antigen - 2c (MSA-2c), Rhoptry Associated Protein - 1 (RAP-1) and Heat Shock Protein 20 (HSP20). Both priming with the adenovirus or recombinant multi-antigen and boosting with the modified vaccinia Ankara vector achieved a high degree of activation of TNFα and IFNγ-secreting CD4(+) and CD8(+) specific T cells 60days after the first immunization. High titers of specific IgG antibodies were also detected at the same time point and lasted up to day 120 of the first immunization. Only the adenovirus - MVA combination triggered a marked isotype skew for the IgG2a antibody subclass meanwhile for the other immune traits analyzed here, both vaccination schemes showed similar performances. The immunological characterization in the murine model of these rationally designed immunogens led us to propose that adenoviruses as well as the bacterially expressed multi-antigen are highly reliable primer candidates to be considered in future experiments in cattle to test protection against bovine babesiosis |
| format | Artículo |
| id | INTA1150 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA11502019-03-22T13:38:30Z Evaluation of different heterologous prime-boost immunization strategies against Babesia bovis using viral vectored and protein-adjuvant vaccines based on a chimeric multi-antigen Jaramillo Ortiz, Jose Manuel Molinari, Maria Paula Gravisaco, María José Paoletta, Martina Montenegro, Valeria Noely Wilkowsky, Silvina Elizabeth Enfermedades de los Animales Babesia Bovis Vacuna Inmunización Animal Diseases Vaccines Immunization Protection against the intraerythrocytic bovine parasite Babesia bovis requires both humoral and cellular immune responses. Therefore, tailored combinations of immunogens targeted at both arms of the immune system are strategies of choice to pursue sterilizing immunity. In this study, different heterologous prime-boost vaccination schemes were evaluated in mice to compare the immunogenicity induced by a recombinant adenovirus, a modified vaccinia Ankara vector or a subunit vaccine all expressing a chimeric multi-antigen. This multi-antigen includes the immunodominant B and T cell epitopes of three B. bovis proteins: Merozoite Surface Antigen - 2c (MSA-2c), Rhoptry Associated Protein - 1 (RAP-1) and Heat Shock Protein 20 (HSP20). Both priming with the adenovirus or recombinant multi-antigen and boosting with the modified vaccinia Ankara vector achieved a high degree of activation of TNFα and IFNγ-secreting CD4(+) and CD8(+) specific T cells 60days after the first immunization. High titers of specific IgG antibodies were also detected at the same time point and lasted up to day 120 of the first immunization. Only the adenovirus - MVA combination triggered a marked isotype skew for the IgG2a antibody subclass meanwhile for the other immune traits analyzed here, both vaccination schemes showed similar performances. The immunological characterization in the murine model of these rationally designed immunogens led us to propose that adenoviruses as well as the bacterially expressed multi-antigen are highly reliable primer candidates to be considered in future experiments in cattle to test protection against bovine babesiosis Inst. de Biotecnología Fil: Jaramillo Ortiz, Jose Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Paoletta, Martina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Montenegro, Valeria Noely. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2017-09-06T14:29:09Z 2017-09-06T14:29:09Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1150 http://www.sciencedirect.com/science/article/pii/S0264410X16303796?via%3Dihub https://link.springer.com/article/10.1007%2Fs00299-016-2026-7 0264-410X https://doi.org/10.1016/j.vaccine.2016.05.053 eng info:eu-repo/semantics/restrictedAccess application/pdf Vaccine 34 (33) : 3913-3919. (July 2016) |
| spellingShingle | Enfermedades de los Animales Babesia Bovis Vacuna Inmunización Animal Diseases Vaccines Immunization Jaramillo Ortiz, Jose Manuel Molinari, Maria Paula Gravisaco, María José Paoletta, Martina Montenegro, Valeria Noely Wilkowsky, Silvina Elizabeth Evaluation of different heterologous prime-boost immunization strategies against Babesia bovis using viral vectored and protein-adjuvant vaccines based on a chimeric multi-antigen |
| title | Evaluation of different heterologous prime-boost immunization strategies against Babesia bovis using viral vectored and protein-adjuvant vaccines based on a chimeric multi-antigen |
| title_full | Evaluation of different heterologous prime-boost immunization strategies against Babesia bovis using viral vectored and protein-adjuvant vaccines based on a chimeric multi-antigen |
| title_fullStr | Evaluation of different heterologous prime-boost immunization strategies against Babesia bovis using viral vectored and protein-adjuvant vaccines based on a chimeric multi-antigen |
| title_full_unstemmed | Evaluation of different heterologous prime-boost immunization strategies against Babesia bovis using viral vectored and protein-adjuvant vaccines based on a chimeric multi-antigen |
| title_short | Evaluation of different heterologous prime-boost immunization strategies against Babesia bovis using viral vectored and protein-adjuvant vaccines based on a chimeric multi-antigen |
| title_sort | evaluation of different heterologous prime boost immunization strategies against babesia bovis using viral vectored and protein adjuvant vaccines based on a chimeric multi antigen |
| topic | Enfermedades de los Animales Babesia Bovis Vacuna Inmunización Animal Diseases Vaccines Immunization |
| url | http://hdl.handle.net/20.500.12123/1150 http://www.sciencedirect.com/science/article/pii/S0264410X16303796?via%3Dihub https://link.springer.com/article/10.1007%2Fs00299-016-2026-7 https://doi.org/10.1016/j.vaccine.2016.05.053 |
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