Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)
Leaf senescence is a complex mechanism ruled by multiple genetic and environmental variables that affect crop yields. It is the last stage in leaf development, is characterized by an active decline in photosynthetic rate, nutrients recycling and cell death. The aim of this work was to identify contr...
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| Format: | info:eu-repo/semantics/article |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1148 http://www.sciencedirect.com/science/article/pii/S0168945216300966?via%3Dihub https://doi.org/10.1016/j.plantsci.2016.05.017 |
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| author | López Gialdi, A.I. Moschen, Sebastian Nicolas Villán, C.S. López Fernández, María Paula Maldonado, Sara Beatriz Paniego, Norma Beatriz Heinz, Ruth Amelia Fernandez, Paula Del Carmen |
| author_browse | Fernandez, Paula Del Carmen Heinz, Ruth Amelia López Fernández, María Paula López Gialdi, A.I. Maldonado, Sara Beatriz Moschen, Sebastian Nicolas Paniego, Norma Beatriz Villán, C.S. |
| author_facet | López Gialdi, A.I. Moschen, Sebastian Nicolas Villán, C.S. López Fernández, María Paula Maldonado, Sara Beatriz Paniego, Norma Beatriz Heinz, Ruth Amelia Fernandez, Paula Del Carmen |
| author_sort | López Gialdi, A.I. |
| collection | INTA Digital |
| description | Leaf senescence is a complex mechanism ruled by multiple genetic and environmental variables that affect crop yields. It is the last stage in leaf development, is characterized by an active decline in photosynthetic rate, nutrients recycling and cell death. The aim of this work was to identify contrasting sunflower inbred lines differing in leaf senescence and to deepen the study of this process in sunflower. Ten sunflower genotypes, previously selected by physiological analysis from 150 inbred genotypes, were evaluated under field conditions through physiological, cytological and molecular analysis. The physiological measurement allowed the identification of two contrasting senescence inbred lines, R453 and B481-6, with an increase in yield in the senescence delayed genotype. These findings were confirmed by cytological and molecular analysis using TUNEL, genomic DNA gel electrophoresis, flow sorting and gene expression analysis by qPCR. These results allowed the selection of the two most promising contrasting genotypes, which enables future studies and the identification of new biomarkers associated to early senescence in sunflower. In addition, they allowed the tuning of cytological techniques for a non-model species and its integration with molecular variables |
| format | info:eu-repo/semantics/article |
| id | INTA1148 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA11482018-06-19T17:46:48Z Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.) López Gialdi, A.I. Moschen, Sebastian Nicolas Villán, C.S. López Fernández, María Paula Maldonado, Sara Beatriz Paniego, Norma Beatriz Heinz, Ruth Amelia Fernandez, Paula Del Carmen Genotipos Helianthus Annuus Avejentamiento Hojas Genotypes Senescence Leaves Girasol Leaf senescence is a complex mechanism ruled by multiple genetic and environmental variables that affect crop yields. It is the last stage in leaf development, is characterized by an active decline in photosynthetic rate, nutrients recycling and cell death. The aim of this work was to identify contrasting sunflower inbred lines differing in leaf senescence and to deepen the study of this process in sunflower. Ten sunflower genotypes, previously selected by physiological analysis from 150 inbred genotypes, were evaluated under field conditions through physiological, cytological and molecular analysis. The physiological measurement allowed the identification of two contrasting senescence inbred lines, R453 and B481-6, with an increase in yield in the senescence delayed genotype. These findings were confirmed by cytological and molecular analysis using TUNEL, genomic DNA gel electrophoresis, flow sorting and gene expression analysis by qPCR. These results allowed the selection of the two most promising contrasting genotypes, which enables future studies and the identification of new biomarkers associated to early senescence in sunflower. In addition, they allowed the tuning of cytological techniques for a non-model species and its integration with molecular variables Inst. de Biotecnología Fil: López Gialdi, A.I. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina Fil: Moschen, Sebastian Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Villán, C.S. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina Fil: López Fernández, María Paula. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad Y Biología Experimental; Argentina Fil: Maldonado, Sara Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biologia Experimental y Aplicada; Argentina Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina 2017-09-06T13:58:47Z 2017-09-06T13:58:47Z 2016 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1148 http://www.sciencedirect.com/science/article/pii/S0168945216300966?via%3Dihub 0168-9452 (Print) 1873-2259 (Online) https://doi.org/10.1016/j.plantsci.2016.05.017 eng info:eu-repo/semantics/restrictedAccess application/pdf Plant science 250 : 40-50. (September 2016) |
| spellingShingle | Genotipos Helianthus Annuus Avejentamiento Hojas Genotypes Senescence Leaves Girasol López Gialdi, A.I. Moschen, Sebastian Nicolas Villán, C.S. López Fernández, María Paula Maldonado, Sara Beatriz Paniego, Norma Beatriz Heinz, Ruth Amelia Fernandez, Paula Del Carmen Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.) |
| title | Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.) |
| title_full | Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.) |
| title_fullStr | Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.) |
| title_full_unstemmed | Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.) |
| title_short | Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.) |
| title_sort | identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies helianthus annuus l |
| topic | Genotipos Helianthus Annuus Avejentamiento Hojas Genotypes Senescence Leaves Girasol |
| url | http://hdl.handle.net/20.500.12123/1148 http://www.sciencedirect.com/science/article/pii/S0168945216300966?via%3Dihub https://doi.org/10.1016/j.plantsci.2016.05.017 |
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