Allelic Variation at Glutenin Loci (Glu-1, Glu-2 and Glu-3) in a Worldwide Durum Wheat Collection and Its Effect on Quality Attributes
Durum wheat grains (Triticum turgidum L. ssp. durum) are the main source for the production of pasta, bread and a variety of products consumed worldwide. The quality of pasta is mainly defined by the rheological properties of gluten, an elastic network in wheat endosperms formed of gliadins and glut...
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| Format: | Artículo |
| Language: | Inglés |
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MDPI
2022
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/11475 https://www.mdpi.com/2304-8158/10/11/2845 https://doi.org/10.3390/foods10112845 |
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| author | Roncallo, Pablo Federico Guzmán, Carlos Larsen, Adelina Olga Achilli, Ana Laura Dreisigacker, Susanne Molfese, Elena Rosa Astiz, Valentina Echenique, Carmen Viviana |
| author_browse | Achilli, Ana Laura Astiz, Valentina Dreisigacker, Susanne Echenique, Carmen Viviana Guzmán, Carlos Larsen, Adelina Olga Molfese, Elena Rosa Roncallo, Pablo Federico |
| author_facet | Roncallo, Pablo Federico Guzmán, Carlos Larsen, Adelina Olga Achilli, Ana Laura Dreisigacker, Susanne Molfese, Elena Rosa Astiz, Valentina Echenique, Carmen Viviana |
| author_sort | Roncallo, Pablo Federico |
| collection | INTA Digital |
| description | Durum wheat grains (Triticum turgidum L. ssp. durum) are the main source for the production of pasta, bread and a variety of products consumed worldwide. The quality of pasta is mainly defined by the rheological properties of gluten, an elastic network in wheat endosperms formed of gliadins and glutenins. In this study, the allelic variation at five glutenin loci was analysed in 196 durum wheat genotypes. Two loci (Glu-A1 and Glu-B1), encoding for high-molecular-weight glutenin subunits (HMW-GS), and three loci (Glu-B2, Glu-A3 and Glu-B3), encoding for low molecular weight glutenin subunits (LMW-GS), were assessed by SDS-PAGE. The SDS-sedimentation test was used and the grain protein content was evaluated. A total of 32 glutenin subunits and 41 glutenin haplotypes were identified. Four novel alleles were detected. Fifteen haplotypes represented 85.7% of glutenin loci variability. Some haplotypes carrying the 7 + 15 and 7 + 22 banding patterns at Glu-B1 showed a high gluten strength similar to those that carried the 7 + 8 or 6 + 8 alleles. A decreasing trend in grain protein content was observed over the last 85 years. Allelic frequencies at the three main loci (Glu-B1, Glu-A3 and Glu-B3) changed over the 1915–2020 period. Gluten strength increased from 1970 to 2020 coinciding with the allelic changes observed. These results offer valuable information for glutenin haplotype-based selection for use in breeding programs. |
| format | Artículo |
| id | INTA11475 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA114752022-03-23T11:59:08Z Allelic Variation at Glutenin Loci (Glu-1, Glu-2 and Glu-3) in a Worldwide Durum Wheat Collection and Its Effect on Quality Attributes Roncallo, Pablo Federico Guzmán, Carlos Larsen, Adelina Olga Achilli, Ana Laura Dreisigacker, Susanne Molfese, Elena Rosa Astiz, Valentina Echenique, Carmen Viviana Trigo Duro Triticum durum Gluteninas Calidad Genética Hard Wheat Glutenins Quality Genetics Durum wheat grains (Triticum turgidum L. ssp. durum) are the main source for the production of pasta, bread and a variety of products consumed worldwide. The quality of pasta is mainly defined by the rheological properties of gluten, an elastic network in wheat endosperms formed of gliadins and glutenins. In this study, the allelic variation at five glutenin loci was analysed in 196 durum wheat genotypes. Two loci (Glu-A1 and Glu-B1), encoding for high-molecular-weight glutenin subunits (HMW-GS), and three loci (Glu-B2, Glu-A3 and Glu-B3), encoding for low molecular weight glutenin subunits (LMW-GS), were assessed by SDS-PAGE. The SDS-sedimentation test was used and the grain protein content was evaluated. A total of 32 glutenin subunits and 41 glutenin haplotypes were identified. Four novel alleles were detected. Fifteen haplotypes represented 85.7% of glutenin loci variability. Some haplotypes carrying the 7 + 15 and 7 + 22 banding patterns at Glu-B1 showed a high gluten strength similar to those that carried the 7 + 8 or 6 + 8 alleles. A decreasing trend in grain protein content was observed over the last 85 years. Allelic frequencies at the three main loci (Glu-B1, Glu-A3 and Glu-B3) changed over the 1915–2020 period. Gluten strength increased from 1970 to 2020 coinciding with the allelic changes observed. These results offer valuable information for glutenin haplotype-based selection for use in breeding programs. EEA Barrow Fil: Roncallo, Pablo F. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Roncallo, Pablo F. Universidad Nacional del Sur. Departamento de Agronomía. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Guzmán, Carlos. Universidad de Córdoba. Escuela Técnica Superior de Ingeniería Agronómica y de Montes. Departamento de Genética; España Fil: Larsen, Adelina Olga. Compañía Molinera del Sur; Argentina Fil: Achilli, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Achilli, Ana Laura. Universidad Nacional del Sur. Departamento de Agronomía. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Dreisigacker, Susanne. International Maize and Wheat Improvement Center (CIMMYT). Global Wheat Program; México Fil: Molfese, Elena Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Barrow; Argentina Fil: Astiz, Valentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cesareo Naredo; Argentina Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina 2022-03-23T11:56:06Z 2022-03-23T11:56:06Z 2021-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11475 https://www.mdpi.com/2304-8158/10/11/2845 2304-8158 https://doi.org/10.3390/foods10112845 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Foods 10 (11) : 2845 (November 2021) |
| spellingShingle | Trigo Duro Triticum durum Gluteninas Calidad Genética Hard Wheat Glutenins Quality Genetics Roncallo, Pablo Federico Guzmán, Carlos Larsen, Adelina Olga Achilli, Ana Laura Dreisigacker, Susanne Molfese, Elena Rosa Astiz, Valentina Echenique, Carmen Viviana Allelic Variation at Glutenin Loci (Glu-1, Glu-2 and Glu-3) in a Worldwide Durum Wheat Collection and Its Effect on Quality Attributes |
| title | Allelic Variation at Glutenin Loci (Glu-1, Glu-2 and Glu-3) in a Worldwide Durum Wheat Collection and Its Effect on Quality Attributes |
| title_full | Allelic Variation at Glutenin Loci (Glu-1, Glu-2 and Glu-3) in a Worldwide Durum Wheat Collection and Its Effect on Quality Attributes |
| title_fullStr | Allelic Variation at Glutenin Loci (Glu-1, Glu-2 and Glu-3) in a Worldwide Durum Wheat Collection and Its Effect on Quality Attributes |
| title_full_unstemmed | Allelic Variation at Glutenin Loci (Glu-1, Glu-2 and Glu-3) in a Worldwide Durum Wheat Collection and Its Effect on Quality Attributes |
| title_short | Allelic Variation at Glutenin Loci (Glu-1, Glu-2 and Glu-3) in a Worldwide Durum Wheat Collection and Its Effect on Quality Attributes |
| title_sort | allelic variation at glutenin loci glu 1 glu 2 and glu 3 in a worldwide durum wheat collection and its effect on quality attributes |
| topic | Trigo Duro Triticum durum Gluteninas Calidad Genética Hard Wheat Glutenins Quality Genetics |
| url | http://hdl.handle.net/20.500.12123/11475 https://www.mdpi.com/2304-8158/10/11/2845 https://doi.org/10.3390/foods10112845 |
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