Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species
Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus. Area of the study: samples were obtained from natural populations of Argentina. Material and Methods: Eleven SSR functional...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/11356 https://revistas.inia.es/index.php/fs/article/view/7188 https://doi.org/10.5424/fs/2015242-07188 |
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| author | Pomponio, Maria Florencia Acuña, Cintia Vanesa Petreath, Vivien Lopez Lauenstein, Diego Marcucci Poltri, Susana Noemi Torales, Susana Leonor |
| author_browse | Acuña, Cintia Vanesa Lopez Lauenstein, Diego Marcucci Poltri, Susana Noemi Petreath, Vivien Pomponio, Maria Florencia Torales, Susana Leonor |
| author_facet | Pomponio, Maria Florencia Acuña, Cintia Vanesa Petreath, Vivien Lopez Lauenstein, Diego Marcucci Poltri, Susana Noemi Torales, Susana Leonor |
| author_sort | Pomponio, Maria Florencia |
| collection | INTA Digital |
| description | Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus.
Area of the study: samples were obtained from natural populations of Argentina.
Material and Methods: Eleven SSR functional markers related to stress and metabolism were amplified in a sample of 152 genotypes from P. alba, P. denudans, P. hassleri P. chilensis, P. flexuosa, and interspecific hybrids.
Main results: In P. alba, the PIC average value was 0.36; and 6 out of the 11 primers showed high values of polymorphism ranging from 0.40 to 0.71. The cross-species transferability was high with high percentages of polymorphic loci.
Research highlights: The SSR markers developed in P.alba were easily transferred to other Prosopis species which did not have functional markers. |
| format | info:ar-repo/semantics/artículo |
| id | INTA11356 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España) |
| publisherStr | Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España) |
| record_format | dspace |
| spelling | INTA113562022-03-09T16:19:35Z Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species Pomponio, Maria Florencia Acuña, Cintia Vanesa Petreath, Vivien Lopez Lauenstein, Diego Marcucci Poltri, Susana Noemi Torales, Susana Leonor Prosopis alba Marcadores Genéticos Prosopis Variación Genética Microsatélites Genetic Markers Genetic Variation Microsatellites Marcadores SSR SSR Markers Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus. Area of the study: samples were obtained from natural populations of Argentina. Material and Methods: Eleven SSR functional markers related to stress and metabolism were amplified in a sample of 152 genotypes from P. alba, P. denudans, P. hassleri P. chilensis, P. flexuosa, and interspecific hybrids. Main results: In P. alba, the PIC average value was 0.36; and 6 out of the 11 primers showed high values of polymorphism ranging from 0.40 to 0.71. The cross-species transferability was high with high percentages of polymorphic loci. Research highlights: The SSR markers developed in P.alba were easily transferred to other Prosopis species which did not have functional markers. Instituto de Recursos Biológicos Fil: Pomponio, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Petreath, Vivien. Universidad Nacional de Patagonia San Juan Bosco. Sede Comodoro Rivadavia; Argentina Fil: Lopez Lauenstein, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Marcucci Poltri, Susana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Torales, Susana Leonor. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina 2022-03-09T16:16:24Z 2022-03-09T16:16:24Z 2015 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11356 https://revistas.inia.es/index.php/fs/article/view/7188 2171-9845 2171-5068 https://doi.org/10.5424/fs/2015242-07188 eng info:eu-repograntAgreement/INTA/PNBIO-1131044/AR./Genómica aplicada a estudios de ecología molecular y diversidad genética. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España) Forest Systems 24 (2) : eRC04 (2015) |
| spellingShingle | Prosopis alba Marcadores Genéticos Prosopis Variación Genética Microsatélites Genetic Markers Genetic Variation Microsatellites Marcadores SSR SSR Markers Pomponio, Maria Florencia Acuña, Cintia Vanesa Petreath, Vivien Lopez Lauenstein, Diego Marcucci Poltri, Susana Noemi Torales, Susana Leonor Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species |
| title | Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species |
| title_full | Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species |
| title_fullStr | Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species |
| title_full_unstemmed | Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species |
| title_short | Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species |
| title_sort | characterization of functional ssr markers in prosopis alba and their transferability across prosopis species |
| topic | Prosopis alba Marcadores Genéticos Prosopis Variación Genética Microsatélites Genetic Markers Genetic Variation Microsatellites Marcadores SSR SSR Markers |
| url | http://hdl.handle.net/20.500.12123/11356 https://revistas.inia.es/index.php/fs/article/view/7188 https://doi.org/10.5424/fs/2015242-07188 |
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