A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile

The tick Amblyomma parvitarsum (Acari: Ixodidae) has established populations in Andean and Patagonic environments of South America. For the present study, adults of A. parvitarsum were collected in highland areas (elevation >3500 m) of Argentina and Chile during 2009–2013, and tested by PCR for rick...

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Autores principales: Ogrzewalska, María, Nieri-Bastos, Fernanda A., Marcili, Arlei, Nava, Santiago, González-Acuña, Daniel, Muñoz-Leal, Sebastian, Ruiz-Arrondo, Ignacio, Venzal, José Manuel, Mangold, Atilio Jose, Labruna, Marcelo B.
Formato: info:eu-repo/semantics/article
Lenguaje:Inglés
Publicado: 2017
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/1134
http://www.sciencedirect.com/science/article/pii/S1877959X16300036?via%3Dihub
https://doi.org/10.1016/j.ttbdis.2016.01.003
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author Ogrzewalska, María
Nieri-Bastos, Fernanda A.
Marcili, Arlei
Nava, Santiago
González-Acuña, Daniel
Muñoz-Leal, Sebastian
Ruiz-Arrondo, Ignacio
Venzal, José Manuel
Mangold, Atilio Jose
Labruna, Marcelo B.
author_browse González-Acuña, Daniel
Labruna, Marcelo B.
Mangold, Atilio Jose
Marcili, Arlei
Muñoz-Leal, Sebastian
Nava, Santiago
Nieri-Bastos, Fernanda A.
Ogrzewalska, María
Ruiz-Arrondo, Ignacio
Venzal, José Manuel
author_facet Ogrzewalska, María
Nieri-Bastos, Fernanda A.
Marcili, Arlei
Nava, Santiago
González-Acuña, Daniel
Muñoz-Leal, Sebastian
Ruiz-Arrondo, Ignacio
Venzal, José Manuel
Mangold, Atilio Jose
Labruna, Marcelo B.
author_sort Ogrzewalska, María
collection INTA Digital
description The tick Amblyomma parvitarsum (Acari: Ixodidae) has established populations in Andean and Patagonic environments of South America. For the present study, adults of A. parvitarsum were collected in highland areas (elevation >3500 m) of Argentina and Chile during 2009–2013, and tested by PCR for rickettsial infection in the laboratory, and isolation of rickettsiae in Vero cell culture by the shell vial technique. Overall, 51 (62.2%) out of 82 A. parvitarsum adult ticks were infected by spotted fever group (SFG) rickettsiae, which generated DNA sequences 100% identical to each other, and when submitted to BLAST analysis, they were 99.3% identical to corresponding sequence of the ompA gene of Rickettsia sp. strain Atlantic rainforest. Rickettsiae were successfully isolated in Vero cell culture from two ticks, one from Argentina and one from Chile. DNA extracted from the third passage of the isolates of Argentina and Chile were processed by PCR, resulting in partial sequences for three rickettsial genes (gltA, ompB, ompA). These sequences were concatenated and aligned with rickettsial corresponding sequences available in GenBank. Phylogenetic analysis revealed that the A. pavitarsum rickettsial agent grouped under high bootstrap support in a clade composed by the SFG pathogens R. sibirica, R. africae, R. parkeri, Rickettsia sp. strain Atlantic rainforest, and two unnamed SFG agents of unknown pathogenicty, Rickettsia sp. strain NOD, and Rickettsia sp. strain ApPR. The pathogenic role of this A. parvitarsum rickettsia cannot be discarded, since several species of tick-borne rickettsiae that were considered nonpathogenic for decades are now associated with human infections.
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spelling INTA11342018-02-21T12:26:08Z A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile Ogrzewalska, María Nieri-Bastos, Fernanda A. Marcili, Arlei Nava, Santiago González-Acuña, Daniel Muñoz-Leal, Sebastian Ruiz-Arrondo, Ignacio Venzal, José Manuel Mangold, Atilio Jose Labruna, Marcelo B. Rickettsia Amblyomma Zona de Montaña Highlands Región Patagónica Amblyomma Parvitarsum The tick Amblyomma parvitarsum (Acari: Ixodidae) has established populations in Andean and Patagonic environments of South America. For the present study, adults of A. parvitarsum were collected in highland areas (elevation >3500 m) of Argentina and Chile during 2009–2013, and tested by PCR for rickettsial infection in the laboratory, and isolation of rickettsiae in Vero cell culture by the shell vial technique. Overall, 51 (62.2%) out of 82 A. parvitarsum adult ticks were infected by spotted fever group (SFG) rickettsiae, which generated DNA sequences 100% identical to each other, and when submitted to BLAST analysis, they were 99.3% identical to corresponding sequence of the ompA gene of Rickettsia sp. strain Atlantic rainforest. Rickettsiae were successfully isolated in Vero cell culture from two ticks, one from Argentina and one from Chile. DNA extracted from the third passage of the isolates of Argentina and Chile were processed by PCR, resulting in partial sequences for three rickettsial genes (gltA, ompB, ompA). These sequences were concatenated and aligned with rickettsial corresponding sequences available in GenBank. Phylogenetic analysis revealed that the A. pavitarsum rickettsial agent grouped under high bootstrap support in a clade composed by the SFG pathogens R. sibirica, R. africae, R. parkeri, Rickettsia sp. strain Atlantic rainforest, and two unnamed SFG agents of unknown pathogenicty, Rickettsia sp. strain NOD, and Rickettsia sp. strain ApPR. The pathogenic role of this A. parvitarsum rickettsia cannot be discarded, since several species of tick-borne rickettsiae that were considered nonpathogenic for decades are now associated with human infections. EEA Rafaela Fil: Ogrzewalska, María. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses; Brasil Fil: Nieri-Bastos, Fernanda A. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil Fil: Marcili, Arlei. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil. Universidade de Santo Amaro. Medicina Veterinária e Bem estar animal; Brasil Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: González-Acuña, Daniel. Universidad de Concepción. Facultad de Ciencias Veterinarias; Chile Fil: Muñoz-Leal, Sebastian. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil. Universidad de Concepción. Facultad de Ciencias Veterinarias; Chile Fil: Ruiz-Arrondo, Ignacio. Universidad de Zaragoza. Departamento de Patología Animal, Facultad de Veterinaria; España Fil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte. Laboratorio de Vectores y enfermedades transmitidas; Uruguay Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Labruna, Marcelo B. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil 2017-09-05T15:32:05Z 2017-09-05T15:32:05Z 2016 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1134 http://www.sciencedirect.com/science/article/pii/S1877959X16300036?via%3Dihub 1877-959X (Print) 1877-9603 (Online) https://doi.org/10.1016/j.ttbdis.2016.01.003 eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) Chile (nation) Ticks and tick-borne diseases 7 (3) : 439-442. (April 2016)
spellingShingle Rickettsia
Amblyomma
Zona de Montaña
Highlands
Región Patagónica
Amblyomma Parvitarsum
Ogrzewalska, María
Nieri-Bastos, Fernanda A.
Marcili, Arlei
Nava, Santiago
González-Acuña, Daniel
Muñoz-Leal, Sebastian
Ruiz-Arrondo, Ignacio
Venzal, José Manuel
Mangold, Atilio Jose
Labruna, Marcelo B.
A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile
title A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile
title_full A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile
title_fullStr A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile
title_full_unstemmed A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile
title_short A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile
title_sort novel spotted fever group rickettsia infecting amblyomma parvitarsum acari ixodidae in highlands of argentina and chile
topic Rickettsia
Amblyomma
Zona de Montaña
Highlands
Región Patagónica
Amblyomma Parvitarsum
url http://hdl.handle.net/20.500.12123/1134
http://www.sciencedirect.com/science/article/pii/S1877959X16300036?via%3Dihub
https://doi.org/10.1016/j.ttbdis.2016.01.003
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