First report of Dactylonectria alcacerensis, Dactylonectria macrodidyma and Ilyonectria liriodendri associated with black-foot disease of grapevine in Argentina
Black-foot is a fungal disease that affects young vines and planting material in the countries where grapevines are cultivated. During the 2018 grape-growing season, symptoms of reduced vigor, short internodes, leaf chlorosis, root rot and necrosis in the base of plants were observed in vines betwee...
| Autores principales: | , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
American Phytopathological Society
2022
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/11336 https://apsjournals.apsnet.org/doi/10.1094/PDIS-10-21-2346-PDN https://doi.org/10.1094/PDIS-10-21-2346-PDN |
| _version_ | 1855036560197550080 |
|---|---|
| author | Longone, Maria Valeria Escoriaza, Maria Georgina Paolinelli, Marcos Gramaje, David |
| author_browse | Escoriaza, Maria Georgina Gramaje, David Longone, Maria Valeria Paolinelli, Marcos |
| author_facet | Longone, Maria Valeria Escoriaza, Maria Georgina Paolinelli, Marcos Gramaje, David |
| author_sort | Longone, Maria Valeria |
| collection | INTA Digital |
| description | Black-foot is a fungal disease that affects young vines and planting material in the countries where grapevines are cultivated. During the 2018 grape-growing season, symptoms of reduced vigor, short internodes, leaf chlorosis, root rot and necrosis in the base of plants were observed in vines between 2 to 10 years old from 400 ha of vineyards of Mendoza and Salta regions. A total of 30% of the vineyards of cultivars Malbec, Cabernet Sauvignon and Aspirant Bouchet, and rootstocks 101-14 Millardet et de Grasset and 1103 Paulsen, had vines with disease symptoms. Vines had 15% disease incidence and 30% disease severity. These symptoms have been described as characteristic of black-foot, caused by "Cylindrocarpon"-like asexual morph fungi. Isolations were made from roots and basal part of 30 symptomatic plants. Fragments of advanced necrotic tissue were washed with running water, surface sterilized with 70% alcohol for 20 seconds, 2% NaOCl solution for 4 minutes and rinsed in sterile distilled water twice. These fragments were placed onto Potato Dextrose Agar (PDA) and incubated at 25°C in the dark for 10 days to isolate fungal pathogens. A total of 50 colonies resembling black-foot disease pathogens were subcultured onto fresh PDA in order to obtain single spore cultures. The isolates developed buff to cinnamon and dark brown, and felty mycelium. Colonies subcultured in synthetic nutrient agar (SNA) produced hyaline, cylindrical, straight and/or slightly curved with one to three septate (35.6 × 5.3 µm) macroconidia and abundant, hyaline, ellipsoidal and zero to one septate (12.5 × 6.0 µm) microconidia. Cultures and conidia morphological characteristics were similar to those of Ilyonectria or Dactylonectria genera (Cabral et al. 2012a; Lombard et al. 2014). DNA sequence analysis of the partial histone H3 gene was obtained for isolates INTA SC1 (I. liriodendri), INTA LC2 (D. alcacerensis) and INTA LC1 (D. macrodidyma) and deposited in GenBank (accession nos. OK338901, OK338900 and OK338899). The BLAST search was conducted against type specimens. Sequences showed high similarity (99% to 100%) to the sequences of Ilyonectria liriodendri (Halleen, Rego & Crous) Chaverri & Salgado (GenBank accession no. JF735509), Dactylonectria alcacerensis (A. Cabral, Oliveira & Crous) L. Lombard & Crous (GenBank accession no. JF735630) and Dactylonectria macrodidyma (Halleen, Schroers & Crous) L. Lombard & Crous (GenBank accession no. JF735647). Pathogenicity tests were conducted under greenhouse conditions on 1-year-old rooted canes cv Malbec using the three isolates. Ten rooted cuttings with pruned roots were immersed in a suspension of 106 conidia ml-1 of each isolate for 60 min (Cabral et al. 2012b) while control cuttings were immersed in sterile distilled water. Immediately they were planted in pots and kept in a greenhouse at a temperature between 25 to 30°C. Symptoms developed on all plants 4 months after inoculation and consisted in necrotic lesions of roots and in the base of the canes, with a reduction in root biomass. Plants did not develop aerial symptoms. All fungi were re-isolated only from necrotic lesions of root and base of inoculated canes, fulfilling Koch's postulates. No symptoms were observed on the control plants. To our knowledge, this is the first report of the species I. liriodendri, D. alcacerensis and D. macrodidyma associated with black-foot disease of grapevine in Argentina. Black-foot disease has a negative effect on the viability of planting material and young vines, and this report will assist with monitoring distribution of the disease as well as developing management recommendations to nurseries and grape growers in Argentina. |
| format | info:ar-repo/semantics/artículo |
| id | INTA11336 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | American Phytopathological Society |
| publisherStr | American Phytopathological Society |
| record_format | dspace |
| spelling | INTA113362022-03-08T17:12:28Z First report of Dactylonectria alcacerensis, Dactylonectria macrodidyma and Ilyonectria liriodendri associated with black-foot disease of grapevine in Argentina Longone, Maria Valeria Escoriaza, Maria Georgina Paolinelli, Marcos Gramaje, David Vid Enfermedades Fungosas Cylindrocarpon Argentina Grapevines Fungal Diseases Enfermedades de la Madera Dactylonectria alcacerensis Dactylonectria macrodidyma Ilyonectria liriodendri Malbec Cabernet Sauvignon Aspirant Bouchet Black-foot is a fungal disease that affects young vines and planting material in the countries where grapevines are cultivated. During the 2018 grape-growing season, symptoms of reduced vigor, short internodes, leaf chlorosis, root rot and necrosis in the base of plants were observed in vines between 2 to 10 years old from 400 ha of vineyards of Mendoza and Salta regions. A total of 30% of the vineyards of cultivars Malbec, Cabernet Sauvignon and Aspirant Bouchet, and rootstocks 101-14 Millardet et de Grasset and 1103 Paulsen, had vines with disease symptoms. Vines had 15% disease incidence and 30% disease severity. These symptoms have been described as characteristic of black-foot, caused by "Cylindrocarpon"-like asexual morph fungi. Isolations were made from roots and basal part of 30 symptomatic plants. Fragments of advanced necrotic tissue were washed with running water, surface sterilized with 70% alcohol for 20 seconds, 2% NaOCl solution for 4 minutes and rinsed in sterile distilled water twice. These fragments were placed onto Potato Dextrose Agar (PDA) and incubated at 25°C in the dark for 10 days to isolate fungal pathogens. A total of 50 colonies resembling black-foot disease pathogens were subcultured onto fresh PDA in order to obtain single spore cultures. The isolates developed buff to cinnamon and dark brown, and felty mycelium. Colonies subcultured in synthetic nutrient agar (SNA) produced hyaline, cylindrical, straight and/or slightly curved with one to three septate (35.6 × 5.3 µm) macroconidia and abundant, hyaline, ellipsoidal and zero to one septate (12.5 × 6.0 µm) microconidia. Cultures and conidia morphological characteristics were similar to those of Ilyonectria or Dactylonectria genera (Cabral et al. 2012a; Lombard et al. 2014). DNA sequence analysis of the partial histone H3 gene was obtained for isolates INTA SC1 (I. liriodendri), INTA LC2 (D. alcacerensis) and INTA LC1 (D. macrodidyma) and deposited in GenBank (accession nos. OK338901, OK338900 and OK338899). The BLAST search was conducted against type specimens. Sequences showed high similarity (99% to 100%) to the sequences of Ilyonectria liriodendri (Halleen, Rego & Crous) Chaverri & Salgado (GenBank accession no. JF735509), Dactylonectria alcacerensis (A. Cabral, Oliveira & Crous) L. Lombard & Crous (GenBank accession no. JF735630) and Dactylonectria macrodidyma (Halleen, Schroers & Crous) L. Lombard & Crous (GenBank accession no. JF735647). Pathogenicity tests were conducted under greenhouse conditions on 1-year-old rooted canes cv Malbec using the three isolates. Ten rooted cuttings with pruned roots were immersed in a suspension of 106 conidia ml-1 of each isolate for 60 min (Cabral et al. 2012b) while control cuttings were immersed in sterile distilled water. Immediately they were planted in pots and kept in a greenhouse at a temperature between 25 to 30°C. Symptoms developed on all plants 4 months after inoculation and consisted in necrotic lesions of roots and in the base of the canes, with a reduction in root biomass. Plants did not develop aerial symptoms. All fungi were re-isolated only from necrotic lesions of root and base of inoculated canes, fulfilling Koch's postulates. No symptoms were observed on the control plants. To our knowledge, this is the first report of the species I. liriodendri, D. alcacerensis and D. macrodidyma associated with black-foot disease of grapevine in Argentina. Black-foot disease has a negative effect on the viability of planting material and young vines, and this report will assist with monitoring distribution of the disease as well as developing management recommendations to nurseries and grape growers in Argentina. EEA Mendoza Fil: Longone, Maria Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Escoriaza, Maria Georgina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Gramaje, David. Universidad de la Rioja. Instituto de Ciencias de la Vid y del Vino; España. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias de la Vid y del Vino; España 2022-03-08T16:46:50Z 2022-03-08T16:46:50Z 2022-03-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11336 https://apsjournals.apsnet.org/doi/10.1094/PDIS-10-21-2346-PDN 1943-7692 0191-2917 https://doi.org/10.1094/PDIS-10-21-2346-PDN eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Phytopathological Society Plant Disease (Published Online:31 Jan 2022) |
| spellingShingle | Vid Enfermedades Fungosas Cylindrocarpon Argentina Grapevines Fungal Diseases Enfermedades de la Madera Dactylonectria alcacerensis Dactylonectria macrodidyma Ilyonectria liriodendri Malbec Cabernet Sauvignon Aspirant Bouchet Longone, Maria Valeria Escoriaza, Maria Georgina Paolinelli, Marcos Gramaje, David First report of Dactylonectria alcacerensis, Dactylonectria macrodidyma and Ilyonectria liriodendri associated with black-foot disease of grapevine in Argentina |
| title | First report of Dactylonectria alcacerensis, Dactylonectria macrodidyma and Ilyonectria liriodendri associated with black-foot disease of grapevine in Argentina |
| title_full | First report of Dactylonectria alcacerensis, Dactylonectria macrodidyma and Ilyonectria liriodendri associated with black-foot disease of grapevine in Argentina |
| title_fullStr | First report of Dactylonectria alcacerensis, Dactylonectria macrodidyma and Ilyonectria liriodendri associated with black-foot disease of grapevine in Argentina |
| title_full_unstemmed | First report of Dactylonectria alcacerensis, Dactylonectria macrodidyma and Ilyonectria liriodendri associated with black-foot disease of grapevine in Argentina |
| title_short | First report of Dactylonectria alcacerensis, Dactylonectria macrodidyma and Ilyonectria liriodendri associated with black-foot disease of grapevine in Argentina |
| title_sort | first report of dactylonectria alcacerensis dactylonectria macrodidyma and ilyonectria liriodendri associated with black foot disease of grapevine in argentina |
| topic | Vid Enfermedades Fungosas Cylindrocarpon Argentina Grapevines Fungal Diseases Enfermedades de la Madera Dactylonectria alcacerensis Dactylonectria macrodidyma Ilyonectria liriodendri Malbec Cabernet Sauvignon Aspirant Bouchet |
| url | http://hdl.handle.net/20.500.12123/11336 https://apsjournals.apsnet.org/doi/10.1094/PDIS-10-21-2346-PDN https://doi.org/10.1094/PDIS-10-21-2346-PDN |
| work_keys_str_mv | AT longonemariavaleria firstreportofdactylonectriaalcacerensisdactylonectriamacrodidymaandilyonectrialiriodendriassociatedwithblackfootdiseaseofgrapevineinargentina AT escoriazamariageorgina firstreportofdactylonectriaalcacerensisdactylonectriamacrodidymaandilyonectrialiriodendriassociatedwithblackfootdiseaseofgrapevineinargentina AT paolinellimarcos firstreportofdactylonectriaalcacerensisdactylonectriamacrodidymaandilyonectrialiriodendriassociatedwithblackfootdiseaseofgrapevineinargentina AT gramajedavid firstreportofdactylonectriaalcacerensisdactylonectriamacrodidymaandilyonectrialiriodendriassociatedwithblackfootdiseaseofgrapevineinargentina |