Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle
Saccharomyces cerevisiae isolates from grapes, soil, vine bark and buds collected at seven phenological stages of an annual growth cycle, were molecular typed by Microsatellite Multiplex PCR. Subsequently 30 S. cerevisiae genotypes were selected and the effect of vineyard environmental stressors, in...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
Elsevier
2022
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/11335 https://www.sciencedirect.com/science/article/pii/S0023643822000925 https://doi.org/10.1016/j.lwt.2022.113157 |
| _version_ | 1855484767995166720 |
|---|---|
| author | Gonzalez, Magali Lucia Rosa Valero, Eva Chimeno, Selva Valeria Garrido Fernandez, Antonio Rodriguez Gomez, Francisco Rojo, Cecilia Paolinelli, Marcos Arroyo Lopez, Francisco Noe Combina, Mariana Mercado, Laura Analia |
| author_browse | Arroyo Lopez, Francisco Noe Chimeno, Selva Valeria Combina, Mariana Garrido Fernandez, Antonio Gonzalez, Magali Lucia Rosa Mercado, Laura Analia Paolinelli, Marcos Rodriguez Gomez, Francisco Rojo, Cecilia Valero, Eva |
| author_facet | Gonzalez, Magali Lucia Rosa Valero, Eva Chimeno, Selva Valeria Garrido Fernandez, Antonio Rodriguez Gomez, Francisco Rojo, Cecilia Paolinelli, Marcos Arroyo Lopez, Francisco Noe Combina, Mariana Mercado, Laura Analia |
| author_sort | Gonzalez, Magali Lucia Rosa |
| collection | INTA Digital |
| description | Saccharomyces cerevisiae isolates from grapes, soil, vine bark and buds collected at seven phenological stages of an annual growth cycle, were molecular typed by Microsatellite Multiplex PCR. Subsequently 30 S. cerevisiae genotypes were selected and the effect of vineyard environmental stressors, in both sublethal upper and lower levels, on their growth parameters was evaluated. The effect of low and high temperature (7–40 ◦C), pH (2.5–8.0), glucose concentration (3.0–300.0 g/L), nitrogen concentration (0.008–8.0 g/L), and copper presence (24 mg/L) were modelled individually using the reparametrized Gompertz equation. Multivariate ANOVA and
Generalized Procrustes Analysis were used to determine the environmental stressor’s influence over the lag phase (λ) and the maximum specific growth rate (μmax). Both parameters were significantly affected by the S. cerevisiae genotype, the treatments, and the interaction between them. Despite a generalized reduction in μmax and a variable answer in λ, the 30 S. cerevisiae genotypes were able to overcome all the treatments. Extreme glucose limitation, copper presence and low temperature had the highest impact over the growth parameters. Interestingly, ten genotypes mostly distributed in the vineyard were the least affected, suggesting a greater acclimatization fitness and the possibility to persist in the changing conditions of the vine annual cycle. |
| format | Artículo |
| id | INTA11335 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA113352022-03-08T16:43:10Z Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle Gonzalez, Magali Lucia Rosa Valero, Eva Chimeno, Selva Valeria Garrido Fernandez, Antonio Rodriguez Gomez, Francisco Rojo, Cecilia Paolinelli, Marcos Arroyo Lopez, Francisco Noe Combina, Mariana Mercado, Laura Analia Vid Levadura Identificación Microsatélites Saccharomyces cerevisiae Grapevines Yeasts Identification Microsatellites Saccharomyces cerevisiae isolates from grapes, soil, vine bark and buds collected at seven phenological stages of an annual growth cycle, were molecular typed by Microsatellite Multiplex PCR. Subsequently 30 S. cerevisiae genotypes were selected and the effect of vineyard environmental stressors, in both sublethal upper and lower levels, on their growth parameters was evaluated. The effect of low and high temperature (7–40 ◦C), pH (2.5–8.0), glucose concentration (3.0–300.0 g/L), nitrogen concentration (0.008–8.0 g/L), and copper presence (24 mg/L) were modelled individually using the reparametrized Gompertz equation. Multivariate ANOVA and Generalized Procrustes Analysis were used to determine the environmental stressor’s influence over the lag phase (λ) and the maximum specific growth rate (μmax). Both parameters were significantly affected by the S. cerevisiae genotype, the treatments, and the interaction between them. Despite a generalized reduction in μmax and a variable answer in λ, the 30 S. cerevisiae genotypes were able to overcome all the treatments. Extreme glucose limitation, copper presence and low temperature had the highest impact over the growth parameters. Interestingly, ten genotypes mostly distributed in the vineyard were the least affected, suggesting a greater acclimatization fitness and the possibility to persist in the changing conditions of the vine annual cycle. EEA Mendoza Fil: Gonzalez, Magali Lucia Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gonzalez, Magali Lucia Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Valero, Eva. Universidad Pablo de Olavide. Departamento de Biología Molecular e Ingeniería Bioquímica; España Fil: Chimeno, Selva Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Garrido Fernandez, Antonio. Universidad Pablo de Olavide. Departamento de Biotecnología de Alimentos, Instituto de la Grasa (IG); España. Consejo Superior de Investigaciones Científicas (CSIC); España Fil: Rodriguez Gomez, Francisco. Universidad Pablo de Olavide. Departamento de Biotecnología de Alimentos, Instituto de la Grasa (IG); España. Consejo Superior de Investigaciones Científicas (CSIC); España Fil: Rojo, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Rojo, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Arroyo Lopez, Francisco Noe. Universidad Pablo de Olavide. Departamento de Biotecnología de Alimentos, Instituto de la Grasa (IG); España. Consejo Superior de Investigaciones Científicas (CSIC); España Fil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Mercado, Laura Analia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina 2022-03-08T16:25:17Z 2022-03-08T16:25:17Z 2022-03-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11335 https://www.sciencedirect.com/science/article/pii/S0023643822000925 0023-6438 https://doi.org/10.1016/j.lwt.2022.113157 eng info:eu-repograntAgreement/INTA/2019-PE-E6-I114-001/2019-PE-E6-I114-001/AR./Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier LWT, Food Science and Technology 158 : 113157 (2022) |
| spellingShingle | Vid Levadura Identificación Microsatélites Saccharomyces cerevisiae Grapevines Yeasts Identification Microsatellites Gonzalez, Magali Lucia Rosa Valero, Eva Chimeno, Selva Valeria Garrido Fernandez, Antonio Rodriguez Gomez, Francisco Rojo, Cecilia Paolinelli, Marcos Arroyo Lopez, Francisco Noe Combina, Mariana Mercado, Laura Analia Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle |
| title | Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle |
| title_full | Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle |
| title_fullStr | Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle |
| title_full_unstemmed | Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle |
| title_short | Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle |
| title_sort | growth response of saccharomyces cerevisiae strains to stressors associated to the vine cycle |
| topic | Vid Levadura Identificación Microsatélites Saccharomyces cerevisiae Grapevines Yeasts Identification Microsatellites |
| url | http://hdl.handle.net/20.500.12123/11335 https://www.sciencedirect.com/science/article/pii/S0023643822000925 https://doi.org/10.1016/j.lwt.2022.113157 |
| work_keys_str_mv | AT gonzalezmagaliluciarosa growthresponseofsaccharomycescerevisiaestrainstostressorsassociatedtothevinecycle AT valeroeva growthresponseofsaccharomycescerevisiaestrainstostressorsassociatedtothevinecycle AT chimenoselvavaleria growthresponseofsaccharomycescerevisiaestrainstostressorsassociatedtothevinecycle AT garridofernandezantonio growthresponseofsaccharomycescerevisiaestrainstostressorsassociatedtothevinecycle AT rodriguezgomezfrancisco growthresponseofsaccharomycescerevisiaestrainstostressorsassociatedtothevinecycle AT rojocecilia growthresponseofsaccharomycescerevisiaestrainstostressorsassociatedtothevinecycle AT paolinellimarcos growthresponseofsaccharomycescerevisiaestrainstostressorsassociatedtothevinecycle AT arroyolopezfrancisconoe growthresponseofsaccharomycescerevisiaestrainstostressorsassociatedtothevinecycle AT combinamariana growthresponseofsaccharomycescerevisiaestrainstostressorsassociatedtothevinecycle AT mercadolauraanalia growthresponseofsaccharomycescerevisiaestrainstostressorsassociatedtothevinecycle |