Integrating transcriptomic and metabolomic analysis to understand natural leaf senescence in sunflower
Leaf senescence is a complex process, which has dramatic consequences on crop yield. In sunflower, gap between potential and actual yields reveals the economic impact of senescence. Indeed, sunflower plants are incapable of maintaining their green leaf area over sustained periods. This study charac...
| Autores principales: | , , , , , , , , , , , , , , , , |
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://onlinelibrary.wiley.com/doi/10.1111/pbi.12422/epdf http://hdl.handle.net/20.500.12123/113 https://doi.org/10.1111/pbi.12422 |
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| author | Moschen, Sebastian Gonzalez, Sergio Alberto Rivarola, Maximo Lisandro Hopp, Horacio Esteban Paniego, Norma Beatriz Fernandez, Paula del Carmen Heinz, Ruth Amelia Bengoa Luoni, Sofia Ailin Di Rienzo, Julio A. Caro, María del Pilar Tohge, Takayuki Watanabe, Mutsumi Hollmann, Julien García-García, Francisco Dopazo, Joaquín Hoefgen, Rainer Fernie, Alisdair R. |
| author_browse | Bengoa Luoni, Sofia Ailin Caro, María del Pilar Di Rienzo, Julio A. Dopazo, Joaquín Fernandez, Paula del Carmen Fernie, Alisdair R. García-García, Francisco Gonzalez, Sergio Alberto Heinz, Ruth Amelia Hoefgen, Rainer Hollmann, Julien Hopp, Horacio Esteban Moschen, Sebastian Paniego, Norma Beatriz Rivarola, Maximo Lisandro Tohge, Takayuki Watanabe, Mutsumi |
| author_facet | Moschen, Sebastian Gonzalez, Sergio Alberto Rivarola, Maximo Lisandro Hopp, Horacio Esteban Paniego, Norma Beatriz Fernandez, Paula del Carmen Heinz, Ruth Amelia Bengoa Luoni, Sofia Ailin Di Rienzo, Julio A. Caro, María del Pilar Tohge, Takayuki Watanabe, Mutsumi Hollmann, Julien García-García, Francisco Dopazo, Joaquín Hoefgen, Rainer Fernie, Alisdair R. |
| author_sort | Moschen, Sebastian |
| collection | INTA Digital |
| description | Leaf senescence is a complex process, which has dramatic consequences on crop yield. In sunflower, gap between potential and actual yields reveals the economic impact of senescence.
Indeed, sunflower plants are incapable of maintaining their green leaf area over sustained periods. This study characterizes the leaf senescence process in sunflower through a systems biology approach integrating transcriptomic and metabolomic analyses: plants being grown under both glasshouse and field conditions. Our results revealed a correspondence between profile changes detected at the molecular, biochemical and physiological level throughout the
progression of leaf senescence measured at different plant developmental stages. Early metabolic changes were detected prior to anthesis and before the onset of the first senescence symptoms, with more pronounced changes observed when physiological and molecular variables were assessed under field conditions. During leaf development, photosynthetic activity and cell growth processes decreased, whereas sucrose, fatty acid, nucleotide and amino acid metabolisms increased. Pathways related to nutrient recycling processes were also up-regulated.
Members of the NAC, AP2-EREBP, HB, bZIP and MYB transcription factor families showed high expression levels, and their expression level was highly correlated, suggesting their involvement in sunflower senescence. The results of this study thus contribute to the elucidation of the molecular mechanisms involved in the onset and progression of leaf senescence in sunflower leaves as well as to the identification of candidate genes involved in this process. |
| format | info:eu-repo/semantics/article |
| id | INTA113 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA1132019-06-05T17:59:57Z Integrating transcriptomic and metabolomic analysis to understand natural leaf senescence in sunflower Moschen, Sebastian Gonzalez, Sergio Alberto Rivarola, Maximo Lisandro Hopp, Horacio Esteban Paniego, Norma Beatriz Fernandez, Paula del Carmen Heinz, Ruth Amelia Bengoa Luoni, Sofia Ailin Di Rienzo, Julio A. Caro, María del Pilar Tohge, Takayuki Watanabe, Mutsumi Hollmann, Julien García-García, Francisco Dopazo, Joaquín Hoefgen, Rainer Fernie, Alisdair R. Genética Girasol Fitomejoramiento Sunflower Genetics Plant Breeding Leaf senescence is a complex process, which has dramatic consequences on crop yield. In sunflower, gap between potential and actual yields reveals the economic impact of senescence. Indeed, sunflower plants are incapable of maintaining their green leaf area over sustained periods. This study characterizes the leaf senescence process in sunflower through a systems biology approach integrating transcriptomic and metabolomic analyses: plants being grown under both glasshouse and field conditions. Our results revealed a correspondence between profile changes detected at the molecular, biochemical and physiological level throughout the progression of leaf senescence measured at different plant developmental stages. Early metabolic changes were detected prior to anthesis and before the onset of the first senescence symptoms, with more pronounced changes observed when physiological and molecular variables were assessed under field conditions. During leaf development, photosynthetic activity and cell growth processes decreased, whereas sucrose, fatty acid, nucleotide and amino acid metabolisms increased. Pathways related to nutrient recycling processes were also up-regulated. Members of the NAC, AP2-EREBP, HB, bZIP and MYB transcription factor families showed high expression levels, and their expression level was highly correlated, suggesting their involvement in sunflower senescence. The results of this study thus contribute to the elucidation of the molecular mechanisms involved in the onset and progression of leaf senescence in sunflower leaves as well as to the identification of candidate genes involved in this process. Inst. de Biotecnología Fil: Moschen, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fernandez, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Bengoa Luoni, Sofía. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina Fil: Di Rienzo, Julio A. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina Fil: Caro, María del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán; Argentina Fil: Tohge, Takayuki. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania Fil: Watanabe, Mutsumi Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania Fil: Hollmann, Julien. University of Kiel. Institute of Botany, Christian-Albrechts; Alemania Fil: García-García, Francisco. Centro de Investigación Príncipe Felipe. Department of Bioinformatics and Genomics; España. Centro de Investigación Príncipe Felipe . National Institute of Bioinformatics. Functional Genomics Node; España Fil: Dopazo, Joaquín. Centro de Investigación Príncipe Felipe. Department of Bioinformatics and Genomics; España. Centro de Investigación Príncipe Felipe . National Institute of Bioinformatics. Functional Genomics Node; España Fil: Hoefgen, Rainer Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania Fil: Fernie, Alisdair R. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania 2017-03-29T15:45:08Z 2017-03-29T15:45:08Z 2016 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:ar-repo/semantics/artículo http://onlinelibrary.wiley.com/doi/10.1111/pbi.12422/epdf http://hdl.handle.net/20.500.12123/113 https://doi.org/10.1111/pbi.12422 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Plant biotechnology journal, 14 (2) : 719-734. (2016 Feb) |
| spellingShingle | Genética Girasol Fitomejoramiento Sunflower Genetics Plant Breeding Moschen, Sebastian Gonzalez, Sergio Alberto Rivarola, Maximo Lisandro Hopp, Horacio Esteban Paniego, Norma Beatriz Fernandez, Paula del Carmen Heinz, Ruth Amelia Bengoa Luoni, Sofia Ailin Di Rienzo, Julio A. Caro, María del Pilar Tohge, Takayuki Watanabe, Mutsumi Hollmann, Julien García-García, Francisco Dopazo, Joaquín Hoefgen, Rainer Fernie, Alisdair R. Integrating transcriptomic and metabolomic analysis to understand natural leaf senescence in sunflower |
| title | Integrating transcriptomic and metabolomic analysis to understand natural leaf senescence in sunflower |
| title_full | Integrating transcriptomic and metabolomic analysis to understand natural leaf senescence in sunflower |
| title_fullStr | Integrating transcriptomic and metabolomic analysis to understand natural leaf senescence in sunflower |
| title_full_unstemmed | Integrating transcriptomic and metabolomic analysis to understand natural leaf senescence in sunflower |
| title_short | Integrating transcriptomic and metabolomic analysis to understand natural leaf senescence in sunflower |
| title_sort | integrating transcriptomic and metabolomic analysis to understand natural leaf senescence in sunflower |
| topic | Genética Girasol Fitomejoramiento Sunflower Genetics Plant Breeding |
| url | http://onlinelibrary.wiley.com/doi/10.1111/pbi.12422/epdf http://hdl.handle.net/20.500.12123/113 https://doi.org/10.1111/pbi.12422 |
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