Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter
Senescence can be delayed in transgenic plants overexpressing the enzyme isopentenyltransferase (IPT) due to stress-induced increased levels of endogenous cytokinins. This trait leads to sustained photo-synthetic activity and improved tolerance to abiotic stress. The aim of this study was to generat...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1124 http://www.sciencedirect.com/science/article/pii/S0168165616300165?via%3Dihub https://doi.org/10.1016/j.jbiotec.2016.01.014 |
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| author | Decima Oneto, Cecilia Andrea Otegui, María Elena Baroli, Irene Beznec, Ailin Faccio, Paula Daniela Bossio, Adrian Ezequiel Blumwald, Eduardo Lewi, Dalia Marcela |
| author_browse | Baroli, Irene Beznec, Ailin Blumwald, Eduardo Bossio, Adrian Ezequiel Decima Oneto, Cecilia Andrea Faccio, Paula Daniela Lewi, Dalia Marcela Otegui, María Elena |
| author_facet | Decima Oneto, Cecilia Andrea Otegui, María Elena Baroli, Irene Beznec, Ailin Faccio, Paula Daniela Bossio, Adrian Ezequiel Blumwald, Eduardo Lewi, Dalia Marcela |
| author_sort | Decima Oneto, Cecilia Andrea |
| collection | INTA Digital |
| description | Senescence can be delayed in transgenic plants overexpressing the enzyme isopentenyltransferase (IPT) due to stress-induced increased levels of endogenous cytokinins. This trait leads to sustained photo-synthetic activity and improved tolerance to abiotic stress. The aim of this study was to generate andcharacterize transgenic plants of maize (Zea mays L.) transformed with the IPT gene sequence underthe regulation of SARK promoter (protein kinase receptor-associated senescence). Three independenttransgenic events and their segregating null controls were evaluated in two watering regimes (WW:well watered; WD: water deficit) imposed for two weeks around anthesis. Our results show that the WD treatment induced IPT expression with the concomitant increase in cytokinin levels, which prolongedthe persistence of total green leaf area, and maintained normal photosynthetic rate and stomatal con-ductance. These trends were accompanied by a minor decrease in number of grains per plant, individualgrain weight and plant grain yield as compared to WW plants. Plants expressing the IPT gene under WD had PGR, anthesis and silking dates and biomass levels similar to WW plants. Our results demonstratethat expression of the IPT gene under the regulation of the SARK promoter helps improve productivity under WD conditions in C4 plants like maize. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1124 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA11242019-01-11T18:19:50Z Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter Decima Oneto, Cecilia Andrea Otegui, María Elena Baroli, Irene Beznec, Ailin Faccio, Paula Daniela Bossio, Adrian Ezequiel Blumwald, Eduardo Lewi, Dalia Marcela Genética Agua Estrés de Sequia Maíz Resistencia a la Sequía Genetics Water Drought Stress Maize Drought Resistance Senescence can be delayed in transgenic plants overexpressing the enzyme isopentenyltransferase (IPT) due to stress-induced increased levels of endogenous cytokinins. This trait leads to sustained photo-synthetic activity and improved tolerance to abiotic stress. The aim of this study was to generate andcharacterize transgenic plants of maize (Zea mays L.) transformed with the IPT gene sequence underthe regulation of SARK promoter (protein kinase receptor-associated senescence). Three independenttransgenic events and their segregating null controls were evaluated in two watering regimes (WW:well watered; WD: water deficit) imposed for two weeks around anthesis. Our results show that the WD treatment induced IPT expression with the concomitant increase in cytokinin levels, which prolongedthe persistence of total green leaf area, and maintained normal photosynthetic rate and stomatal con-ductance. These trends were accompanied by a minor decrease in number of grains per plant, individualgrain weight and plant grain yield as compared to WW plants. Plants expressing the IPT gene under WD had PGR, anthesis and silking dates and biomass levels similar to WW plants. Our results demonstratethat expression of the IPT gene under the regulation of the SARK promoter helps improve productivity under WD conditions in C4 plants like maize. Inst. de Genética "Ewald A. Favret"- IGEAF Fil: Decima Oneto, Cecilia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Otegui, María Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina Fil: Baroli, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Bossio, Adrian EzequielInstituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Blumwald, Eduardo. University of California. Department Plant Sciences; Estados Unidos Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina 2017-09-05T12:41:08Z 2017-09-05T12:41:08Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/acceptedVersion info:eu-repo/semantics/article http://hdl.handle.net/20.500.12123/1124 http://www.sciencedirect.com/science/article/pii/S0168165616300165?via%3Dihub 0168-1656 (Print) 1873-4863 (Online) https://doi.org/10.1016/j.jbiotec.2016.01.014 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of biotechnology 220 : 66-77. (February 2016) |
| spellingShingle | Genética Agua Estrés de Sequia Maíz Resistencia a la Sequía Genetics Water Drought Stress Maize Drought Resistance Decima Oneto, Cecilia Andrea Otegui, María Elena Baroli, Irene Beznec, Ailin Faccio, Paula Daniela Bossio, Adrian Ezequiel Blumwald, Eduardo Lewi, Dalia Marcela Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter |
| title | Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter |
| title_full | Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter |
| title_fullStr | Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter |
| title_full_unstemmed | Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter |
| title_short | Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter |
| title_sort | water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase ipt gene driven by a stress and maturation induced promoter |
| topic | Genética Agua Estrés de Sequia Maíz Resistencia a la Sequía Genetics Water Drought Stress Maize Drought Resistance |
| url | http://hdl.handle.net/20.500.12123/1124 http://www.sciencedirect.com/science/article/pii/S0168165616300165?via%3Dihub https://doi.org/10.1016/j.jbiotec.2016.01.014 |
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