Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines
Background: Anaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hip...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | info:eu-repo/semantics/article |
| Language: | Inglés |
| Published: |
2017
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/1117 https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1555-9 https://doi.org/10.1186/s13071-016-1555-9 |
| _version_ | 1855034750992908288 |
|---|---|
| author | Guillemi, Eliana Carolina De La Fourniere, Sofia Ana María Orozco, Marcela Peña Martínez, Jorge Correa, Elena Fernández, Javier Lopez Arias, Ludmila Sol Paoletta, Martina Corona, Belkis Pinarello, Valérie Wilkowsky, Silvina Elizabeth Farber, Marisa Diana |
| author_browse | Corona, Belkis Correa, Elena De La Fourniere, Sofia Ana María Farber, Marisa Diana Fernández, Javier Guillemi, Eliana Carolina Lopez Arias, Ludmila Sol Orozco, Marcela Paoletta, Martina Peña Martínez, Jorge Pinarello, Valérie Wilkowsky, Silvina Elizabeth |
| author_facet | Guillemi, Eliana Carolina De La Fourniere, Sofia Ana María Orozco, Marcela Peña Martínez, Jorge Correa, Elena Fernández, Javier Lopez Arias, Ludmila Sol Paoletta, Martina Corona, Belkis Pinarello, Valérie Wilkowsky, Silvina Elizabeth Farber, Marisa Diana |
| author_sort | Guillemi, Eliana Carolina |
| collection | INTA Digital |
| description | Background: Anaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hippocamelus antisense (taruca), which are two native endangered species.
Methods: Samples from two sick wild animals: a Myrmecophaga tridactyla (blood) and a Hippocamelus antisense (blood and serum) were studied for the presence of A. marginale DNA through msp5 gene fragment amplification.
Further characterization was done through MSP1a tandem repeats analysis and MLST scheme and the genetic relationship among previously characterized A. marginale sequences were studied by applying, eBURST algorithm
and AMOVA analysis.
Results: Anaplasma marginale DNA was identified in the Myrmecophaga tridactyla and Hippocamelus antisense samples. Through molecular markers, we identified an identical genotype in both animals that was not previously reported in bovine host. The analysis through eBURST and AMOVA revealed no differentiation between the taruca/ anteater isolate and the bovine group. |
| format | info:eu-repo/semantics/article |
| id | INTA1117 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA11172019-01-23T17:25:04Z Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines Guillemi, Eliana Carolina De La Fourniere, Sofia Ana María Orozco, Marcela Peña Martínez, Jorge Correa, Elena Fernández, Javier Lopez Arias, Ludmila Sol Paoletta, Martina Corona, Belkis Pinarello, Valérie Wilkowsky, Silvina Elizabeth Farber, Marisa Diana Genética Anaplasma Marginale Genotipos Ganado Bovino Genetics Genotypes Cattle América del Sur Background: Anaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hippocamelus antisense (taruca), which are two native endangered species. Methods: Samples from two sick wild animals: a Myrmecophaga tridactyla (blood) and a Hippocamelus antisense (blood and serum) were studied for the presence of A. marginale DNA through msp5 gene fragment amplification. Further characterization was done through MSP1a tandem repeats analysis and MLST scheme and the genetic relationship among previously characterized A. marginale sequences were studied by applying, eBURST algorithm and AMOVA analysis. Results: Anaplasma marginale DNA was identified in the Myrmecophaga tridactyla and Hippocamelus antisense samples. Through molecular markers, we identified an identical genotype in both animals that was not previously reported in bovine host. The analysis through eBURST and AMOVA revealed no differentiation between the taruca/ anteater isolate and the bovine group. Inst. de Biotecnología Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: De La Fourniere, Sofia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Orozco, Maria Marcela. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Ecologia, Genetica y Evolucion. Laboratorio de Eco - Epidemiologia; Consejo Nacional de Invest. Científ.y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina; Fil: Peña Martínez, Jorge. Conservation Land Trust; Argentina Fil: Correa, Elena. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales. Reserva Experimental Horco Molle; Argentina Fil: Fernández, Javier. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales. Reserva Experimental Horco Molle; Argentina Fil: Lopez Arias, Ludmila Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Paoletta, Martina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Corona, Belkis. Centro Nacional de Sanidad Agropecuaria; Cuba Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Pinarello, Valérie. Institut National de la Recherche Agronomique. Contrôle des Maladies Animales Exotiques et Emergentes Centre; Francia 2017-09-04T17:38:39Z 2017-09-04T17:38:39Z 2016 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1117 https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1555-9 1756-3305 (Online) https://doi.org/10.1186/s13071-016-1555-9 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Parasites & Vectors 169 : 305 (2016) |
| spellingShingle | Genética Anaplasma Marginale Genotipos Ganado Bovino Genetics Genotypes Cattle América del Sur Guillemi, Eliana Carolina De La Fourniere, Sofia Ana María Orozco, Marcela Peña Martínez, Jorge Correa, Elena Fernández, Javier Lopez Arias, Ludmila Sol Paoletta, Martina Corona, Belkis Pinarello, Valérie Wilkowsky, Silvina Elizabeth Farber, Marisa Diana Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
| title | Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
| title_full | Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
| title_fullStr | Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
| title_full_unstemmed | Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
| title_short | Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
| title_sort | molecular identification of anaplasma marginale in two autochthonous south american wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
| topic | Genética Anaplasma Marginale Genotipos Ganado Bovino Genetics Genotypes Cattle América del Sur |
| url | http://hdl.handle.net/20.500.12123/1117 https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1555-9 https://doi.org/10.1186/s13071-016-1555-9 |
| work_keys_str_mv | AT guillemielianacarolina molecularidentificationofanaplasmamarginaleintwoautochthonoussouthamericanwildspeciesrevealedanidenticalnewgenotypeanditsphylogeneticrelationshipwiththoseofbovines AT delafournieresofiaanamaria molecularidentificationofanaplasmamarginaleintwoautochthonoussouthamericanwildspeciesrevealedanidenticalnewgenotypeanditsphylogeneticrelationshipwiththoseofbovines AT orozcomarcela molecularidentificationofanaplasmamarginaleintwoautochthonoussouthamericanwildspeciesrevealedanidenticalnewgenotypeanditsphylogeneticrelationshipwiththoseofbovines AT penamartinezjorge molecularidentificationofanaplasmamarginaleintwoautochthonoussouthamericanwildspeciesrevealedanidenticalnewgenotypeanditsphylogeneticrelationshipwiththoseofbovines AT correaelena molecularidentificationofanaplasmamarginaleintwoautochthonoussouthamericanwildspeciesrevealedanidenticalnewgenotypeanditsphylogeneticrelationshipwiththoseofbovines AT fernandezjavier molecularidentificationofanaplasmamarginaleintwoautochthonoussouthamericanwildspeciesrevealedanidenticalnewgenotypeanditsphylogeneticrelationshipwiththoseofbovines AT lopezariasludmilasol molecularidentificationofanaplasmamarginaleintwoautochthonoussouthamericanwildspeciesrevealedanidenticalnewgenotypeanditsphylogeneticrelationshipwiththoseofbovines AT paolettamartina molecularidentificationofanaplasmamarginaleintwoautochthonoussouthamericanwildspeciesrevealedanidenticalnewgenotypeanditsphylogeneticrelationshipwiththoseofbovines AT coronabelkis molecularidentificationofanaplasmamarginaleintwoautochthonoussouthamericanwildspeciesrevealedanidenticalnewgenotypeanditsphylogeneticrelationshipwiththoseofbovines AT pinarellovalerie molecularidentificationofanaplasmamarginaleintwoautochthonoussouthamericanwildspeciesrevealedanidenticalnewgenotypeanditsphylogeneticrelationshipwiththoseofbovines AT wilkowskysilvinaelizabeth molecularidentificationofanaplasmamarginaleintwoautochthonoussouthamericanwildspeciesrevealedanidenticalnewgenotypeanditsphylogeneticrelationshipwiththoseofbovines AT farbermarisadiana molecularidentificationofanaplasmamarginaleintwoautochthonoussouthamericanwildspeciesrevealedanidenticalnewgenotypeanditsphylogeneticrelationshipwiththoseofbovines |