The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes
Cercospora leaf blight (CLB) causes extensive losses in soybean production in worldwide, including major soybean-producing countries such as Argentina. Cercospora kikuchii, C. cf. sigesbeckiae, C. cf. flagellaris, and C. cf. nicotianae are identified as pathogens of CLB. Soybean resistance against C...
| Main Authors: | , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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The American Phytopathological Society (APS)
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/11162 https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-01-21-0002-TA https://doi.org/10.1094/PHYTOFR-01-21-0002-TA |
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| author | Kashiwa, Takeshi Lavilla, Miguel Angel Diaz Paleo, Antonio Horacio Ivancovich, Antonio Juan Gerardo Yamanaka, Naoki |
| author_browse | Diaz Paleo, Antonio Horacio Ivancovich, Antonio Juan Gerardo Kashiwa, Takeshi Lavilla, Miguel Angel Yamanaka, Naoki |
| author_facet | Kashiwa, Takeshi Lavilla, Miguel Angel Diaz Paleo, Antonio Horacio Ivancovich, Antonio Juan Gerardo Yamanaka, Naoki |
| author_sort | Kashiwa, Takeshi |
| collection | INTA Digital |
| description | Cercospora leaf blight (CLB) causes extensive losses in soybean production in worldwide, including major soybean-producing countries such as Argentina. Cercospora kikuchii, C. cf. sigesbeckiae, C. cf. flagellaris, and C. cf. nicotianae are identified as pathogens of CLB. Soybean resistance against CLB is still unknown. Also, chemical control for CLB is losing effectiveness because of fungicide resistance of pathogens such as C. kikuchii. We urgently need to breed a CLB-resistant cultivar. Unfortunately, efficient methods for the screening of a resistant soybean genotype have not yet been established. In this study, we designed a new, high-throughput inoculation method for identifying resistance against one of the CLB pathogens, C. kikuchii. We used liquid-cultured mycelia of the pathogen C. kikuchii on detached soybean leaves. Lesions on soybean leaflets appeared 9 days postinoculation by this method. We used this method to select four C. kikuchii-resistant genotypes from 80 genotypes in the World Soybean Core Collection. The high-throughput screening method developed in this study can contribute to the research about C. kikuchii resistance by facilitating identification of resistant varieties. |
| format | Artículo |
| id | INTA11162 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | The American Phytopathological Society (APS) |
| publisherStr | The American Phytopathological Society (APS) |
| record_format | dspace |
| spelling | INTA111622022-02-16T11:33:46Z The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes Kashiwa, Takeshi Lavilla, Miguel Angel Diaz Paleo, Antonio Horacio Ivancovich, Antonio Juan Gerardo Yamanaka, Naoki Soja Inoculación Métodos de Aplicación Genotipos Cercospora kikuchii Organismos Patógenos Soybeans Inoculation Application Methods Genotypes Pathogens Cercospora Leaf Blight Cercospora leaf blight (CLB) causes extensive losses in soybean production in worldwide, including major soybean-producing countries such as Argentina. Cercospora kikuchii, C. cf. sigesbeckiae, C. cf. flagellaris, and C. cf. nicotianae are identified as pathogens of CLB. Soybean resistance against CLB is still unknown. Also, chemical control for CLB is losing effectiveness because of fungicide resistance of pathogens such as C. kikuchii. We urgently need to breed a CLB-resistant cultivar. Unfortunately, efficient methods for the screening of a resistant soybean genotype have not yet been established. In this study, we designed a new, high-throughput inoculation method for identifying resistance against one of the CLB pathogens, C. kikuchii. We used liquid-cultured mycelia of the pathogen C. kikuchii on detached soybean leaves. Lesions on soybean leaflets appeared 9 days postinoculation by this method. We used this method to select four C. kikuchii-resistant genotypes from 80 genotypes in the World Soybean Core Collection. The high-throughput screening method developed in this study can contribute to the research about C. kikuchii resistance by facilitating identification of resistant varieties. EEA Pergamino Fil: Kashiwa, Takeshi. Japan International Research Center for Agricultural Sciences (JIRCAS). Biological Resources and Post-harvest Division; Japón Fil: Lavilla, Miguel Ángel. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina Fil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Laboratorio Biotecnología; Argentina Fil: Diaz Paleo, Antonio Horacio. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina Fil: Ivancovich, Antonio Juan Gerardo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina Fil: Yamanaka, Naoki. Japan International Research Center for Agricultural Sciences (JIRCAS). Biological Resources and Post-harvest Division; Japón 2022-02-16T11:25:11Z 2022-02-16T11:25:11Z 2021-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11162 https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-01-21-0002-TA 2690-5442 https://doi.org/10.1094/PHYTOFR-01-21-0002-TA eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf The American Phytopathological Society (APS) PhytoFrontiers 1 (4) : 250-257. (November 2021) |
| spellingShingle | Soja Inoculación Métodos de Aplicación Genotipos Cercospora kikuchii Organismos Patógenos Soybeans Inoculation Application Methods Genotypes Pathogens Cercospora Leaf Blight Kashiwa, Takeshi Lavilla, Miguel Angel Diaz Paleo, Antonio Horacio Ivancovich, Antonio Juan Gerardo Yamanaka, Naoki The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes |
| title | The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes |
| title_full | The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes |
| title_fullStr | The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes |
| title_full_unstemmed | The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes |
| title_short | The Use of Detached Leaf Inoculation for Selecting Cercospora kikuchii Resistance in Soybean Genotypes |
| title_sort | use of detached leaf inoculation for selecting cercospora kikuchii resistance in soybean genotypes |
| topic | Soja Inoculación Métodos de Aplicación Genotipos Cercospora kikuchii Organismos Patógenos Soybeans Inoculation Application Methods Genotypes Pathogens Cercospora Leaf Blight |
| url | http://hdl.handle.net/20.500.12123/11162 https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-01-21-0002-TA https://doi.org/10.1094/PHYTOFR-01-21-0002-TA |
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