Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers
The live attenuated Brucella abortus SRB51 (SRB51) is a partial O-chain-deprived mutant. The relative lack of the polysaccharide prevents it from inducing antibodies detectable by most of the serological tests used for the diagnosis of bovine brucellosis. The performance of three antigens used in an...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/11134 https://www.sciencedirect.com/science/article/abs/pii/S0165242708003486 https://doi.org/10.1016/j.vetimm.2008.09.007 |
| _version_ | 1855484726476800000 |
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| author | Robles, Carlos Alejandro Nielsen, Klaus Gall, David Willems, Priscila Mabel |
| author_browse | Gall, David Nielsen, Klaus Robles, Carlos Alejandro Willems, Priscila Mabel |
| author_facet | Robles, Carlos Alejandro Nielsen, Klaus Gall, David Willems, Priscila Mabel |
| author_sort | Robles, Carlos Alejandro |
| collection | INTA Digital |
| description | The live attenuated Brucella abortus SRB51 (SRB51) is a partial O-chain-deprived mutant. The relative lack of the polysaccharide prevents it from inducing antibodies detectable by most of the serological tests used for the diagnosis of bovine brucellosis. The performance of three antigens used in an indirect enzyme-linked immunoassay test for detecting SRB51 antibodies were evaluated. A homogeneous group of twenty-five 10-month-old Hereford heifers was used. The animals were bled on day 0 and then subcutaneously vaccinated with 2 ml of a commercially available SRB51 vaccine (Schering-PloughTM) containing 1 107 to 3.4 107 viable cells. Blood samples without anticoagulant for sera obtaining were then collected at days 30, 90, 210 and 360 post-vaccination. To detect the SRB51 antibodies, Brucella ovis hot saline extract, B. ovis RLPS (RLPS), and SRB51-RLPS were used. The buffered antigen plate agglutination test and an indirect enzyme-linked immunoassay (I-ELISA) using the smooth LPS (SLPS) antigen from B. abortus were used as control tests. All the sera samples were negative in the BPA test and in the standard I-ELISA using the SLPS. The SRB51-RLPS and the B. ovis RLPS antigens performed better than the B. ovis hot saline extract antigen. |
| format | Artículo |
| id | INTA11134 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA111342022-02-14T11:51:21Z Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers Robles, Carlos Alejandro Nielsen, Klaus Gall, David Willems, Priscila Mabel Brucella Brucella abortus Brucelosis Antígenos Anticuerpos Técnicas Inmunológicas Ganado Bovino Brucellosis Antigens Antibodies Immunological Techniques Cattle Región Patagónica Inmunoensayo The live attenuated Brucella abortus SRB51 (SRB51) is a partial O-chain-deprived mutant. The relative lack of the polysaccharide prevents it from inducing antibodies detectable by most of the serological tests used for the diagnosis of bovine brucellosis. The performance of three antigens used in an indirect enzyme-linked immunoassay test for detecting SRB51 antibodies were evaluated. A homogeneous group of twenty-five 10-month-old Hereford heifers was used. The animals were bled on day 0 and then subcutaneously vaccinated with 2 ml of a commercially available SRB51 vaccine (Schering-PloughTM) containing 1 107 to 3.4 107 viable cells. Blood samples without anticoagulant for sera obtaining were then collected at days 30, 90, 210 and 360 post-vaccination. To detect the SRB51 antibodies, Brucella ovis hot saline extract, B. ovis RLPS (RLPS), and SRB51-RLPS were used. The buffered antigen plate agglutination test and an indirect enzyme-linked immunoassay (I-ELISA) using the smooth LPS (SLPS) antigen from B. abortus were used as control tests. All the sera samples were negative in the BPA test and in the standard I-ELISA using the SLPS. The SRB51-RLPS and the B. ovis RLPS antigens performed better than the B. ovis hot saline extract antigen. Estación Experimental Agropecuaria Bariloche Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina Fil: Nielsen, Klaus. Canadian Food Inspection Agency. Ottawa Laboratories; Canadá Fil: Gall, David. Canadian Food Inspection Agency. Ottawa Laboratories; Canadá Fil: Willems, Priscila Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina 2022-02-14T11:44:37Z 2022-02-14T11:44:37Z 2009 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11134 https://www.sciencedirect.com/science/article/abs/pii/S0165242708003486 0165-2427 https://doi.org/10.1016/j.vetimm.2008.09.007 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Immunology and Immunopathology 127 : 153-155 (2009) |
| spellingShingle | Brucella Brucella abortus Brucelosis Antígenos Anticuerpos Técnicas Inmunológicas Ganado Bovino Brucellosis Antigens Antibodies Immunological Techniques Cattle Región Patagónica Inmunoensayo Robles, Carlos Alejandro Nielsen, Klaus Gall, David Willems, Priscila Mabel Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers |
| title | Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers |
| title_full | Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers |
| title_fullStr | Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers |
| title_full_unstemmed | Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers |
| title_short | Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers |
| title_sort | evaluation of three different antigens in an indirect enzyme linked immunoassay for the detection of antibodies against brucella abortus srb51 in vaccinated heifers |
| topic | Brucella Brucella abortus Brucelosis Antígenos Anticuerpos Técnicas Inmunológicas Ganado Bovino Brucellosis Antigens Antibodies Immunological Techniques Cattle Región Patagónica Inmunoensayo |
| url | http://hdl.handle.net/20.500.12123/11134 https://www.sciencedirect.com/science/article/abs/pii/S0165242708003486 https://doi.org/10.1016/j.vetimm.2008.09.007 |
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