Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina
Ascochyta blight is one of the most devastating foliar diseases of chickpea. The causal agent, Ascochyta rabiei, is a heterothallic ascomycete and its sexual reproduction depends on the proximate presence of both mating types, MAT1-1 and MAT1-2. Since its first detection in Argentina in 2011, very f...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Wiley
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/11131 https://onlinelibrary.wiley.com/doi/10.1111/jph.13073 https://doi.org/10.1111/jph.13073 |
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| author | Crociara, Clara Sonia Valetti, Lucio Bernanrdi Lima, Nelson Iglesias, Juliana Pastor, Silvina |
| author_browse | Bernanrdi Lima, Nelson Crociara, Clara Sonia Iglesias, Juliana Pastor, Silvina Valetti, Lucio |
| author_facet | Crociara, Clara Sonia Valetti, Lucio Bernanrdi Lima, Nelson Iglesias, Juliana Pastor, Silvina |
| author_sort | Crociara, Clara Sonia |
| collection | INTA Digital |
| description | Ascochyta blight is one of the most devastating foliar diseases of chickpea. The causal agent, Ascochyta rabiei, is a heterothallic ascomycete and its sexual reproduction depends on the proximate presence of both mating types, MAT1-1 and MAT1-2. Since its first detection in Argentina in 2011, very few studies have been carried out and information on local isolates remains unknown. In this work, 12 isolates were obtained from the northern, central and southern regions of Córdoba province, Argentina. First, isolates were tentatively identified as A. rabiei based on their colony and conidial characteristics. Identity was confirmed by phylogenetic analysis of the ITS, β-tubulin and D1/D2 loci. Morphology, cultural characteristics and pathogenic variability of the isolates were compared. Likewise, 27 crosses were made to demonstrate possible sexual reproduction between the strains. The isolates showed differences in morphology (shape, texture, conidial size, colony colour, growth rate) and aggressiveness from a susceptible cultivar. All crosses were fertile and produced pseudothecia, asci and ascospores. This work is the first in Latin America to characterize isolates of A. rabiei morphologically and molecularly, examine their pathogenic variability and demonstrate the sexual reproduction between isolates carrying different mating-type idiomorphs. New levels of aggressiveness in A. rabiei or adaptive changes like resistance to fungicides or breakdown of host resistance are a distinct possibility in the future. |
| format | info:ar-repo/semantics/artículo |
| id | INTA11131 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA111312022-02-14T10:50:30Z Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina Crociara, Clara Sonia Valetti, Lucio Bernanrdi Lima, Nelson Iglesias, Juliana Pastor, Silvina Enfermedades de las Plantas Ascochyta rabiei Tizón Garbanzo Enfermedades Foliares Fitopatología Plant Diseases Blight Chickpeas Foliar Diseases Plant Pathology Didymella rabiei Pathogenic Variability Mating types Ascochyta blight is one of the most devastating foliar diseases of chickpea. The causal agent, Ascochyta rabiei, is a heterothallic ascomycete and its sexual reproduction depends on the proximate presence of both mating types, MAT1-1 and MAT1-2. Since its first detection in Argentina in 2011, very few studies have been carried out and information on local isolates remains unknown. In this work, 12 isolates were obtained from the northern, central and southern regions of Córdoba province, Argentina. First, isolates were tentatively identified as A. rabiei based on their colony and conidial characteristics. Identity was confirmed by phylogenetic analysis of the ITS, β-tubulin and D1/D2 loci. Morphology, cultural characteristics and pathogenic variability of the isolates were compared. Likewise, 27 crosses were made to demonstrate possible sexual reproduction between the strains. The isolates showed differences in morphology (shape, texture, conidial size, colony colour, growth rate) and aggressiveness from a susceptible cultivar. All crosses were fertile and produced pseudothecia, asci and ascospores. This work is the first in Latin America to characterize isolates of A. rabiei morphologically and molecularly, examine their pathogenic variability and demonstrate the sexual reproduction between isolates carrying different mating-type idiomorphs. New levels of aggressiveness in A. rabiei or adaptive changes like resistance to fungicides or breakdown of host resistance are a distinct possibility in the future. EEA Pergamino Fil: Crociara, Clara Sonia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Crociara, Clara Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Bernardi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Bernardi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Iglesias, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Departamento de Maíz; Argentina Fil: Iglesias, Juliana. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Agrarias, Naturales y Ambientales; Argentina Fil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Pastor, Silvina Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina 2022-02-14T10:43:31Z 2022-02-14T10:43:31Z 2022-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11131 https://onlinelibrary.wiley.com/doi/10.1111/jph.13073 1439-0434 (online) https://doi.org/10.1111/jph.13073 eng info:eu-repograntAgreement/INTA/2019-PE-E6-I132-001/2019-PE-E6-I132-001/AR./Desarrollo de legumbres adaptadas a los territorios y diferenciados por calidad comercial, nutricional e industrial. info:eu-repograntAgreement/INTA/2019-PD-E4-I090-001/2019-PD-E4-I090-001/AR./Análisis de patosistemas en cultivos agrícolas y especies forestales. Caracterización de sus componentes info:eu-repo/semantics/restrictedAccess application/pdf Wiley Journal of Phytopathology 170 (1) : 1-12. (January 2022) |
| spellingShingle | Enfermedades de las Plantas Ascochyta rabiei Tizón Garbanzo Enfermedades Foliares Fitopatología Plant Diseases Blight Chickpeas Foliar Diseases Plant Pathology Didymella rabiei Pathogenic Variability Mating types Crociara, Clara Sonia Valetti, Lucio Bernanrdi Lima, Nelson Iglesias, Juliana Pastor, Silvina Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina |
| title | Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina |
| title_full | Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina |
| title_fullStr | Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina |
| title_full_unstemmed | Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina |
| title_short | Morphological and molecular characterization, pathogenicity and sexual reproduction of Ascochyta rabiei isolates of chickpea fields in Argentina |
| title_sort | morphological and molecular characterization pathogenicity and sexual reproduction of ascochyta rabiei isolates of chickpea fields in argentina |
| topic | Enfermedades de las Plantas Ascochyta rabiei Tizón Garbanzo Enfermedades Foliares Fitopatología Plant Diseases Blight Chickpeas Foliar Diseases Plant Pathology Didymella rabiei Pathogenic Variability Mating types |
| url | http://hdl.handle.net/20.500.12123/11131 https://onlinelibrary.wiley.com/doi/10.1111/jph.13073 https://doi.org/10.1111/jph.13073 |
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