Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
Uruguay is a leading exporter of bovine meat and dairy products, and cattle production is one of the principal economic backbones in this country. A main clinical problem faced by livestock farmers is neonatal calf diarrhea (NCD); however, causes of NCD have not been extensively studied in Uruguay....
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/11125 https://www.sciencedirect.com/science/article/abs/pii/S1567134820304445 https://doi.org/10.1016/j.meegid.2020.104613 |
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| author | Castells, Matias Caffarena, Rubén Darío Casaux, María Laura Schild, Carlos Castells, Felipe Castells, Daniel Victoria, Matías Riet‑Correa, Franklin Giannitti, Federico Parreño, Viviana Colina, Rodney |
| author_browse | Caffarena, Rubén Darío Casaux, María Laura Castells, Daniel Castells, Felipe Castells, Matias Colina, Rodney Giannitti, Federico Parreño, Viviana Riet‑Correa, Franklin Schild, Carlos Victoria, Matías |
| author_facet | Castells, Matias Caffarena, Rubén Darío Casaux, María Laura Schild, Carlos Castells, Felipe Castells, Daniel Victoria, Matías Riet‑Correa, Franklin Giannitti, Federico Parreño, Viviana Colina, Rodney |
| author_sort | Castells, Matias |
| collection | INTA Digital |
| description | Uruguay is a leading exporter of bovine meat and dairy products, and cattle production is one of the principal economic backbones in this country. A main clinical problem faced by livestock farmers is neonatal calf diarrhea (NCD); however, causes of NCD have not been extensively studied in Uruguay. Bovine norovirus (BoNoV) has been proposed as one of the possible etiologies of NCD as experimentally infected calves developed diarrhea and enteropathy, although limited information is available from field surveys. The aims of this study were to determine the frequency of infection, to investigate possible risk factors, and to determine the molecular diversity of BoNoV in Uruguay. A total of 761 samples of feces or intestinal contents from dairy and beef calves were analyzed through RT-qPCR. The overall frequency of detection of BoNoV was 66.1% with higher frequency in dairy (70.5%) than beef (15.9%) calves (p < 0.01). BoNoV was detected similarly in diarrheic (78.8%) and non-diarrheic (76.2%) dairy calves (p = 0.50). Calves ≤2 weeks of age (84%) were infected more often than older (62.7%) calves (p < 0.01). Phylogenetic analysis confirmed the presence of GIII.1 and GIII.2 genotypes. In addition, we reported the circulation of recombinant strains and the detection of a strain with the recently described novel VP1 genotype. This study represents the first report describing the circulation, the associated risk factors, and the molecular diversity of BoNoV in Uruguay. |
| format | Artículo |
| id | INTA11125 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA111252022-01-14T13:57:56Z Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay Castells, Matias Caffarena, Rubén Darío Casaux, María Laura Schild, Carlos Castells, Felipe Castells, Daniel Victoria, Matías Riet‑Correa, Franklin Giannitti, Federico Parreño, Viviana Colina, Rodney Ganado Bovino Virus de los Animales Genotipos Uruguay Cattle Animal Viruses Genotypes Norovirus Bovino Bovine Norovirus Uruguay is a leading exporter of bovine meat and dairy products, and cattle production is one of the principal economic backbones in this country. A main clinical problem faced by livestock farmers is neonatal calf diarrhea (NCD); however, causes of NCD have not been extensively studied in Uruguay. Bovine norovirus (BoNoV) has been proposed as one of the possible etiologies of NCD as experimentally infected calves developed diarrhea and enteropathy, although limited information is available from field surveys. The aims of this study were to determine the frequency of infection, to investigate possible risk factors, and to determine the molecular diversity of BoNoV in Uruguay. A total of 761 samples of feces or intestinal contents from dairy and beef calves were analyzed through RT-qPCR. The overall frequency of detection of BoNoV was 66.1% with higher frequency in dairy (70.5%) than beef (15.9%) calves (p < 0.01). BoNoV was detected similarly in diarrheic (78.8%) and non-diarrheic (76.2%) dairy calves (p = 0.50). Calves ≤2 weeks of age (84%) were infected more often than older (62.7%) calves (p < 0.01). Phylogenetic analysis confirmed the presence of GIII.1 and GIII.2 genotypes. In addition, we reported the circulation of recombinant strains and the detection of a strain with the recently described novel VP1 genotype. This study represents the first report describing the circulation, the associated risk factors, and the molecular diversity of BoNoV in Uruguay. Instituto de Virología Fil: Castells, Matías. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay. Fil: Castells, Matías. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay Fil: Caffarena, Rubén Darío. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay Fil: Caffarena, Rubén Darío. Universidad de la República. Facultad de Veterinaria; Uruguay. Fil: Casaux, María Laura. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay Fil: Schild, Carlos. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay Fil: Castells, Felipe. Profesional independiente; Uruguay Fil: Castells, Daniel. Secretariado Uruguayo de la Lana. Centro de Investigación y Experimentación Dr. Alejandro Gallinal; Uruguay. Fil: Victoria, Matías. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay. Fil: Riet‑Correa, Franklin. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay Fil: Giannitti, Federico. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Fil: Colina, Rodney. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay. 2022-01-14T13:55:17Z 2022-01-14T13:55:17Z 2020-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/11125 https://www.sciencedirect.com/science/article/abs/pii/S1567134820304445 1567-1348 https://doi.org/10.1016/j.meegid.2020.104613 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Infection, Genetics and Evolution 86 : 104613 (December 2020) |
| spellingShingle | Ganado Bovino Virus de los Animales Genotipos Uruguay Cattle Animal Viruses Genotypes Norovirus Bovino Bovine Norovirus Castells, Matias Caffarena, Rubén Darío Casaux, María Laura Schild, Carlos Castells, Felipe Castells, Daniel Victoria, Matías Riet‑Correa, Franklin Giannitti, Federico Parreño, Viviana Colina, Rodney Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay |
| title | Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay |
| title_full | Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay |
| title_fullStr | Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay |
| title_full_unstemmed | Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay |
| title_short | Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay |
| title_sort | detection risk factors and molecular diversity of norovirus giii in cattle in uruguay |
| topic | Ganado Bovino Virus de los Animales Genotipos Uruguay Cattle Animal Viruses Genotypes Norovirus Bovino Bovine Norovirus |
| url | http://hdl.handle.net/20.500.12123/11125 https://www.sciencedirect.com/science/article/abs/pii/S1567134820304445 https://doi.org/10.1016/j.meegid.2020.104613 |
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