In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina
In Argentina, the cattle tick Rhipicephalus microplus has already developed resistance to organophospates and synthetic pyrethroids. However, no cases of amitraz resistance have ever been recorded in this country despite its heavy use. A recent failure of amitraz to control ticks in a farm located i...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2022
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/11123 https://www.sciencedirect.com/science/article/abs/pii/S0304401712005614 https://doi.org/10.1016/j.vetpar.2012.10.014 |
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| author | Cutulle, Christian Lovis, Léonore D’Agostino, Beatriz Inés Balbiani, Gabriel Morici, Gabriel Edgardo Citroni, Daniel Reggi, Julio Caracostantogolo, Jorge Luis |
| author_browse | Balbiani, Gabriel Caracostantogolo, Jorge Luis Citroni, Daniel Cutulle, Christian D’Agostino, Beatriz Inés Lovis, Léonore Morici, Gabriel Edgardo Reggi, Julio |
| author_facet | Cutulle, Christian Lovis, Léonore D’Agostino, Beatriz Inés Balbiani, Gabriel Morici, Gabriel Edgardo Citroni, Daniel Reggi, Julio Caracostantogolo, Jorge Luis |
| author_sort | Cutulle, Christian |
| collection | INTA Digital |
| description | In Argentina, the cattle tick Rhipicephalus microplus has already developed resistance to organophospates and synthetic pyrethroids. However, no cases of amitraz resistance have ever been recorded in this country despite its heavy use. A recent failure of amitraz to control ticks in a farm located in Santo Tomé, province of Corrientes, resulted in the collection of samples for acaricide resistance diagnosis. The modified Drummond adult immersion test (AIT) and the larval tarsal test (LTT) were performed separately in Argentina and Switzerland to evaluate efficacy of amitraz and other acaricides. The AIT showed that oviposition in the Santo Tomé field isolate was not inhibited when it was challenged to 250 and 500 ppm amitraz, and 50 ppm deltamethrin. However, oviposition was reduced by 90.6% when this field isolate was challenged to a combination of 400 ppm ethion and 100 ppm cypermethrin. To confirm the results obtained with the AIT, 2 additional tick samples were collected and shipped to Switzerland for resistance diagnosis of amitraz, cypermethrin and flumethrin, using the LTT. With this bioassay, the resistance ratios of the 2 field isolates were 32.5 and 57.0 for amitraz and between 5.9 and 27.2 for the synthetic pyrethroids. Both in vitro bioassays confirmed amitraz and synthetic pyrethroid resistance in the Santo Tomé samples. These results account for the first evidence of amitraz resistance in R. microplus in Argentina. |
| format | info:ar-repo/semantics/artículo |
| id | INTA11123 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA111232022-01-14T12:14:25Z In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina Cutulle, Christian Lovis, Léonore D’Agostino, Beatriz Inés Balbiani, Gabriel Morici, Gabriel Edgardo Citroni, Daniel Reggi, Julio Caracostantogolo, Jorge Luis Rhipicephalus Control de Plagas Resistencia a los Acaricidas Experimentación in Vitro Ganado Bovino Argentina Amitraz Pest Control Resistance to Acaricides In Vitro Experimentation Cattle Garrapatas Rhipicephalus microplus Santo Tomé, Corrientes Ticks In Argentina, the cattle tick Rhipicephalus microplus has already developed resistance to organophospates and synthetic pyrethroids. However, no cases of amitraz resistance have ever been recorded in this country despite its heavy use. A recent failure of amitraz to control ticks in a farm located in Santo Tomé, province of Corrientes, resulted in the collection of samples for acaricide resistance diagnosis. The modified Drummond adult immersion test (AIT) and the larval tarsal test (LTT) were performed separately in Argentina and Switzerland to evaluate efficacy of amitraz and other acaricides. The AIT showed that oviposition in the Santo Tomé field isolate was not inhibited when it was challenged to 250 and 500 ppm amitraz, and 50 ppm deltamethrin. However, oviposition was reduced by 90.6% when this field isolate was challenged to a combination of 400 ppm ethion and 100 ppm cypermethrin. To confirm the results obtained with the AIT, 2 additional tick samples were collected and shipped to Switzerland for resistance diagnosis of amitraz, cypermethrin and flumethrin, using the LTT. With this bioassay, the resistance ratios of the 2 field isolates were 32.5 and 57.0 for amitraz and between 5.9 and 27.2 for the synthetic pyrethroids. Both in vitro bioassays confirmed amitraz and synthetic pyrethroid resistance in the Santo Tomé samples. These results account for the first evidence of amitraz resistance in R. microplus in Argentina. Instituto de Patobiología Fil: Cutulle, Christian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina Fil: Lovis, Léonore. of Neuchâtel. Institute of Biology. Laboratory of Parasitology; Suiza Fil: D’Agostino, Beatriz Inés. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina Fil: Balbiani, Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina Fil: Morici, Gabriel Edgardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Citroni, Daniel. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina Fil: Reggi, Julio. Novartis Animal Health Argentina; Argentina Fil: Caracostantogolo, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina 2022-01-14T12:12:03Z 2022-01-14T12:12:03Z 2013-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11123 https://www.sciencedirect.com/science/article/abs/pii/S0304401712005614 0304-4017 https://doi.org/10.1016/j.vetpar.2012.10.014 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Parasitology 192 (1-3) : 296-300 (February 2013) |
| spellingShingle | Rhipicephalus Control de Plagas Resistencia a los Acaricidas Experimentación in Vitro Ganado Bovino Argentina Amitraz Pest Control Resistance to Acaricides In Vitro Experimentation Cattle Garrapatas Rhipicephalus microplus Santo Tomé, Corrientes Ticks Cutulle, Christian Lovis, Léonore D’Agostino, Beatriz Inés Balbiani, Gabriel Morici, Gabriel Edgardo Citroni, Daniel Reggi, Julio Caracostantogolo, Jorge Luis In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina |
| title | In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina |
| title_full | In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina |
| title_fullStr | In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina |
| title_full_unstemmed | In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina |
| title_short | In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina |
| title_sort | in vitro diagnosis of the first case of amitraz resistance in rhipicephalus microplus in santo tome corrientes argentina |
| topic | Rhipicephalus Control de Plagas Resistencia a los Acaricidas Experimentación in Vitro Ganado Bovino Argentina Amitraz Pest Control Resistance to Acaricides In Vitro Experimentation Cattle Garrapatas Rhipicephalus microplus Santo Tomé, Corrientes Ticks |
| url | http://hdl.handle.net/20.500.12123/11123 https://www.sciencedirect.com/science/article/abs/pii/S0304401712005614 https://doi.org/10.1016/j.vetpar.2012.10.014 |
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