Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina
Cercospora species cause cercospora leaf blight (CLB) and purple seed stain (PSS) on soybean. Because there are few resistant soybean varieties available, CLB/PSS management relies heavily upon fungicide applications. Sensitivity of 62 Argentinian Cercospora isolates to demethylation inhibitor (DMI)...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/11121 https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13261 https://doi.org/10.1111/ppa.13261 |
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| author | Sautua, Francisco José Doyle, Vinson P. Price, Paul P. Porfiri, Alejandro Fernandez, Paula Del Carmen Scandiani, María Mercedes Carmona, Marcelo Anibal |
| author_browse | Carmona, Marcelo Anibal Doyle, Vinson P. Fernandez, Paula Del Carmen Porfiri, Alejandro Price, Paul P. Sautua, Francisco José Scandiani, María Mercedes |
| author_facet | Sautua, Francisco José Doyle, Vinson P. Price, Paul P. Porfiri, Alejandro Fernandez, Paula Del Carmen Scandiani, María Mercedes Carmona, Marcelo Anibal |
| author_sort | Sautua, Francisco José |
| collection | INTA Digital |
| description | Cercospora species cause cercospora leaf blight (CLB) and purple seed stain (PSS) on soybean. Because there are few resistant soybean varieties available, CLB/PSS management relies heavily upon fungicide applications. Sensitivity of 62 Argentinian Cercospora isolates to demethylation inhibitor (DMI), methyl benzimidazole carbamate (MBC), quinone outside inhibitor (QoI), succinate dehydrogenase inhibitor (SDHI) fungicides, and mancozeb was determined in this study. All isolates were sensitive to difenoconazole, epoxiconazole, prothioconazole, tebuconazole, and cyproconazole (EC50 values ranged from 0.006 to 2.4 µg/ml). In contrast, 51% of the tested isolates were sensitive (EC50 values ranged from 0.003 to 0.2 µg/ml), and 49% were highly resistant (EC50 > 100 µg/ml) to carbendazim. Interestingly, all isolates were completely resistant to azoxystrobin, trifloxystrobin, and pyraclostrobin, and insensitive to boscalid, fluxapyroxad, and pydiflumetofen (EC50 > 100 µg/ml). The G143A mutation was detected in 82% (53) of the QoI-resistant isolates and the E198A mutation in 97% (31) of the carbendazim-resistant isolates. No apparent resistance mutations were detected in the succinate dehydrogenase genes (subunits sdhB, sdhC, and sdhD). Mancozeb completely inhibited mycelial growth of the isolates evaluated at a concentration of 100 µg/ml. All Argentinian Cercospora isolates were sensitive to the DMI fungicides tested, but we report for the first time resistance to QoI and MBC fungicides. Mechanism(s) other than fungicide target-site modification may be responsible for resistance of Cercospora to QoI and MBC fungicides. Moreover, based on our results and on the recent introduction of SDHI fungicides on soybean in Argentina, Cercospora species causing CLB/PSS are insensitive (naturally resistant) to SDHI fungicides. Insensitivity must be confirmed under field conditions. |
| format | info:ar-repo/semantics/artículo |
| id | INTA11121 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA111212022-01-14T11:22:38Z Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina Sautua, Francisco José Doyle, Vinson P. Price, Paul P. Porfiri, Alejandro Fernandez, Paula Del Carmen Scandiani, María Mercedes Carmona, Marcelo Anibal Cercospora Fungicidas Resistencia a los Fungicidas Soja Enfermedades de las Plantas Fungicides Resistance to Fungicides Soybeans Plant Diseases Argentina Cercospora species cause cercospora leaf blight (CLB) and purple seed stain (PSS) on soybean. Because there are few resistant soybean varieties available, CLB/PSS management relies heavily upon fungicide applications. Sensitivity of 62 Argentinian Cercospora isolates to demethylation inhibitor (DMI), methyl benzimidazole carbamate (MBC), quinone outside inhibitor (QoI), succinate dehydrogenase inhibitor (SDHI) fungicides, and mancozeb was determined in this study. All isolates were sensitive to difenoconazole, epoxiconazole, prothioconazole, tebuconazole, and cyproconazole (EC50 values ranged from 0.006 to 2.4 µg/ml). In contrast, 51% of the tested isolates were sensitive (EC50 values ranged from 0.003 to 0.2 µg/ml), and 49% were highly resistant (EC50 > 100 µg/ml) to carbendazim. Interestingly, all isolates were completely resistant to azoxystrobin, trifloxystrobin, and pyraclostrobin, and insensitive to boscalid, fluxapyroxad, and pydiflumetofen (EC50 > 100 µg/ml). The G143A mutation was detected in 82% (53) of the QoI-resistant isolates and the E198A mutation in 97% (31) of the carbendazim-resistant isolates. No apparent resistance mutations were detected in the succinate dehydrogenase genes (subunits sdhB, sdhC, and sdhD). Mancozeb completely inhibited mycelial growth of the isolates evaluated at a concentration of 100 µg/ml. All Argentinian Cercospora isolates were sensitive to the DMI fungicides tested, but we report for the first time resistance to QoI and MBC fungicides. Mechanism(s) other than fungicide target-site modification may be responsible for resistance of Cercospora to QoI and MBC fungicides. Moreover, based on our results and on the recent introduction of SDHI fungicides on soybean in Argentina, Cercospora species causing CLB/PSS are insensitive (naturally resistant) to SDHI fungicides. Insensitivity must be confirmed under field conditions. Instituto de Biotecnología Fil: Sautua, Francisco José. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Fitopatología; Argentina Fil: Doyle, Vinson P. LSU AgCenter. Department of Plant Pathology and Crop Physiology; Estados Unidos Fil: Price, Paul P. LSU AgCenter. Macon Ridge Research Station; Estados Unidos Fil: Porfiri, Alejandro. Consultor independiente; Argentina Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Fernandez, Paula Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Scandiani, María Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Referencia de Micología (CEREMIC); Argentina Fil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Fitopatología; Argentina 2022-01-14T11:19:52Z 2022-01-14T11:19:52Z 2020-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11121 https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13261 0032-0862 1365-3059 https://doi.org/10.1111/ppa.13261 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Plant Pathology 69 (9) : 1678-1694 (December 2020) |
| spellingShingle | Cercospora Fungicidas Resistencia a los Fungicidas Soja Enfermedades de las Plantas Fungicides Resistance to Fungicides Soybeans Plant Diseases Argentina Sautua, Francisco José Doyle, Vinson P. Price, Paul P. Porfiri, Alejandro Fernandez, Paula Del Carmen Scandiani, María Mercedes Carmona, Marcelo Anibal Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina |
| title | Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina |
| title_full | Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina |
| title_fullStr | Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina |
| title_full_unstemmed | Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina |
| title_short | Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina |
| title_sort | fungicide resistance in cercospora species causing cercospora leaf blight and purple seed stain of soybean in argentina |
| topic | Cercospora Fungicidas Resistencia a los Fungicidas Soja Enfermedades de las Plantas Fungicides Resistance to Fungicides Soybeans Plant Diseases Argentina |
| url | http://hdl.handle.net/20.500.12123/11121 https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13261 https://doi.org/10.1111/ppa.13261 |
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