Upregulation of interferon-alpha gene in bovine embryos produced in vitro in response to experimental infection with noncytophatic bovine-viral-diarrhea virus
In-vitro fertilization is a routine livestock-breeding technique widely used around the world. Several studies have reported the interaction of bovine viral-diarrhea virus (BVDV) with gametes and in-vitro-produced (IVP) bovine embryos. Since, gene expression in BVDV-infected IVP bovine embryos is sc...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/11115 https://link.springer.com/article/10.1007%2Fs11033-020-05958-7 https://doi.org/10.1007/s11033-020-05958-7 |
| _version_ | 1855484718370258944 |
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| author | González Altamiranda, Erika Arias, María E. Kaiser, German Gustavo Mucci, Nicolas Crescencio Odeon, Anselmo Carlos Felmer, Ricardo N. |
| author_browse | Arias, María E. Felmer, Ricardo N. González Altamiranda, Erika Kaiser, German Gustavo Mucci, Nicolas Crescencio Odeon, Anselmo Carlos |
| author_facet | González Altamiranda, Erika Arias, María E. Kaiser, German Gustavo Mucci, Nicolas Crescencio Odeon, Anselmo Carlos Felmer, Ricardo N. |
| author_sort | González Altamiranda, Erika |
| collection | INTA Digital |
| description | In-vitro fertilization is a routine livestock-breeding technique widely used around the world. Several studies have reported the interaction of bovine viral-diarrhea virus (BVDV) with gametes and in-vitro-produced (IVP) bovine embryos. Since, gene expression in BVDV-infected IVP bovine embryos is scarcely addressed. The aim of this work was to evaluate the differential expression of genes involved in immune and inflammatory response. Groups of 20–25 embryos on Day 6 (morula stage) were exposed (infected) or not (control) to an NCP-BVDV strain in SOF medium. After 24 h, embryos that reached expanded blastocyst stage were washed. Total RNA of each embryo group was extracted to determine the transcription levels of 9 specific transcripts related with antiviral and inflammatory response by SYBR Green real time quantitative (RT-qPCR). Culture media and an aliquot of the last embryos wash on Day 7 were analyzed by titration and virus isolation, respectively. A conventional PCR confirmed BVDV presence in IVP embryos. A significantly higher expression of interferon-α was observed in blastocysts exposed to NCP-BVDV compared to the controls (p < 0.05). In this study, the upregulation of INFα and TLR7 genes involved in inflammatory and immune response in BVDV-infected IVP bovine embryos is a new finding in this field. This differential expression suggest that embryonic cells could function in a manner like immune cells by recognizing and responding early to interaction with viral pathogens. These results provide new insights into the action of BVDV on the complex molecular pathways controlling bovine early embryonic development. |
| format | Artículo |
| id | INTA11115 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA111152022-01-13T12:54:42Z Upregulation of interferon-alpha gene in bovine embryos produced in vitro in response to experimental infection with noncytophatic bovine-viral-diarrhea virus González Altamiranda, Erika Arias, María E. Kaiser, German Gustavo Mucci, Nicolas Crescencio Odeon, Anselmo Carlos Felmer, Ricardo N. Ganado Bovino Embriones Animales Infección Experimental Pestivirus de la Diarrea Bovina Experimentación in Vitro Cattle Animal Embryos Experimental Infection Bovine Diarrhoa Pestivirus In Vitro Experimentation In-vitro fertilization is a routine livestock-breeding technique widely used around the world. Several studies have reported the interaction of bovine viral-diarrhea virus (BVDV) with gametes and in-vitro-produced (IVP) bovine embryos. Since, gene expression in BVDV-infected IVP bovine embryos is scarcely addressed. The aim of this work was to evaluate the differential expression of genes involved in immune and inflammatory response. Groups of 20–25 embryos on Day 6 (morula stage) were exposed (infected) or not (control) to an NCP-BVDV strain in SOF medium. After 24 h, embryos that reached expanded blastocyst stage were washed. Total RNA of each embryo group was extracted to determine the transcription levels of 9 specific transcripts related with antiviral and inflammatory response by SYBR Green real time quantitative (RT-qPCR). Culture media and an aliquot of the last embryos wash on Day 7 were analyzed by titration and virus isolation, respectively. A conventional PCR confirmed BVDV presence in IVP embryos. A significantly higher expression of interferon-α was observed in blastocysts exposed to NCP-BVDV compared to the controls (p < 0.05). In this study, the upregulation of INFα and TLR7 genes involved in inflammatory and immune response in BVDV-infected IVP bovine embryos is a new finding in this field. This differential expression suggest that embryonic cells could function in a manner like immune cells by recognizing and responding early to interaction with viral pathogens. These results provide new insights into the action of BVDV on the complex molecular pathways controlling bovine early embryonic development. Instituto de Virología Fil: Gonzalez Altamiranda, Erika. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: González Altamiranda, Erika. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Arias, María E. Universidad de La Frontera. Facultad de Ciencias Agrícolas y Forestales. Laboratorio de Reproducción Centro de Biotecnologia Reproductiva CEBIOR-BIOREN; Chile Fil: Kaiser, German Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Kaiser, German Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Mucci, Nicolas Crescencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Mucci, Nicolas Crescencio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Odeón, Anselmo Carlos. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Felmer, Ricardo N. Universidad de La Frontera. Facultad de Ciencias Agrícolas y Forestales. Laboratorio de Reproducción Centro de Biotecnologia Reproductiva CEBIOR-BIOREN; Chile 2022-01-13T12:51:15Z 2022-01-13T12:51:15Z 2020-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11115 https://link.springer.com/article/10.1007%2Fs11033-020-05958-7 0301-4851 1573-4978 https://doi.org/10.1007/s11033-020-05958-7 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Molecular Biology Reports 47 : 9959–9965 (2020) |
| spellingShingle | Ganado Bovino Embriones Animales Infección Experimental Pestivirus de la Diarrea Bovina Experimentación in Vitro Cattle Animal Embryos Experimental Infection Bovine Diarrhoa Pestivirus In Vitro Experimentation González Altamiranda, Erika Arias, María E. Kaiser, German Gustavo Mucci, Nicolas Crescencio Odeon, Anselmo Carlos Felmer, Ricardo N. Upregulation of interferon-alpha gene in bovine embryos produced in vitro in response to experimental infection with noncytophatic bovine-viral-diarrhea virus |
| title | Upregulation of interferon-alpha gene in bovine embryos produced in vitro in response to experimental infection with noncytophatic bovine-viral-diarrhea virus |
| title_full | Upregulation of interferon-alpha gene in bovine embryos produced in vitro in response to experimental infection with noncytophatic bovine-viral-diarrhea virus |
| title_fullStr | Upregulation of interferon-alpha gene in bovine embryos produced in vitro in response to experimental infection with noncytophatic bovine-viral-diarrhea virus |
| title_full_unstemmed | Upregulation of interferon-alpha gene in bovine embryos produced in vitro in response to experimental infection with noncytophatic bovine-viral-diarrhea virus |
| title_short | Upregulation of interferon-alpha gene in bovine embryos produced in vitro in response to experimental infection with noncytophatic bovine-viral-diarrhea virus |
| title_sort | upregulation of interferon alpha gene in bovine embryos produced in vitro in response to experimental infection with noncytophatic bovine viral diarrhea virus |
| topic | Ganado Bovino Embriones Animales Infección Experimental Pestivirus de la Diarrea Bovina Experimentación in Vitro Cattle Animal Embryos Experimental Infection Bovine Diarrhoa Pestivirus In Vitro Experimentation |
| url | http://hdl.handle.net/20.500.12123/11115 https://link.springer.com/article/10.1007%2Fs11033-020-05958-7 https://doi.org/10.1007/s11033-020-05958-7 |
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