PsAA9A, a C1-specific AA9 lytic polysaccharide monooxygenase from the white-rot basidiomycete Pycnoporus sanguineus
Woody biomass represents an important source of carbon on earth, and its global recycling is highly dependent on Agaricomycetes fungi. White-rot Basidiomycetes are a very important group in this regard, as they possess a large and diverse enzymatic repertoire for biomass decomposition. Among these e...
| Main Authors: | , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/11114 https://link.springer.com/article/10.1007%2Fs00253-020-10911-6 https://doi.org/10.1007/s00253-020-10911-6 |
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| author | Garrido, Mercedes Maria Landoni, Malena Sabbadin, Federico Valacco, María Pía Couto, Alicia Bruce, Neil Charles Wirth, Sonia Alejandra Campos, Eleonora |
| author_browse | Bruce, Neil Charles Campos, Eleonora Couto, Alicia Garrido, Mercedes Maria Landoni, Malena Sabbadin, Federico Valacco, María Pía Wirth, Sonia Alejandra |
| author_facet | Garrido, Mercedes Maria Landoni, Malena Sabbadin, Federico Valacco, María Pía Couto, Alicia Bruce, Neil Charles Wirth, Sonia Alejandra Campos, Eleonora |
| author_sort | Garrido, Mercedes Maria |
| collection | INTA Digital |
| description | Woody biomass represents an important source of carbon on earth, and its global recycling is highly dependent on Agaricomycetes fungi. White-rot Basidiomycetes are a very important group in this regard, as they possess a large and diverse enzymatic repertoire for biomass decomposition. Among these enzymes, the recently discovered lytic polysaccharide monooxygenases (LPMOs) have revolutionized biomass processing with their novel oxidative mechanism of action. The strikingly high representation of LPMOs in fungal genomes raises the question of their functional versatility. In this work, we studied an AA9 LPMO from the white-rot basidiomycete Pycnoporus sanguineus, PsAA9A. Successfully produced as a recombinant secreted protein in Pichia pastoris, PsAA9A was found to be a C1-specific LPMO active on cellulosic substrates, generating native and oxidized cello-oligosaccharides in the presence of an external electron donor. PsAA9A boosted cellulolytic activity of glysoside hydrolases from families GH1, GH5, and GH6.This study serves as a starting point towards understanding the functional versatility and biotechnological potential of this enzymatic family, highly represented in wood decay fungi, in Pycnoporus genus. |
| format | Artículo |
| id | INTA11114 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA111142022-01-13T12:22:38Z PsAA9A, a C1-specific AA9 lytic polysaccharide monooxygenase from the white-rot basidiomycete Pycnoporus sanguineus Garrido, Mercedes Maria Landoni, Malena Sabbadin, Federico Valacco, María Pía Couto, Alicia Bruce, Neil Charles Wirth, Sonia Alejandra Campos, Eleonora Pycnoporus Celulosa Oligosacáridos Biomasa Cellulose Oligosaccharides Biomass cientifico Pycnoporus sanguineus Woody biomass represents an important source of carbon on earth, and its global recycling is highly dependent on Agaricomycetes fungi. White-rot Basidiomycetes are a very important group in this regard, as they possess a large and diverse enzymatic repertoire for biomass decomposition. Among these enzymes, the recently discovered lytic polysaccharide monooxygenases (LPMOs) have revolutionized biomass processing with their novel oxidative mechanism of action. The strikingly high representation of LPMOs in fungal genomes raises the question of their functional versatility. In this work, we studied an AA9 LPMO from the white-rot basidiomycete Pycnoporus sanguineus, PsAA9A. Successfully produced as a recombinant secreted protein in Pichia pastoris, PsAA9A was found to be a C1-specific LPMO active on cellulosic substrates, generating native and oxidized cello-oligosaccharides in the presence of an external electron donor. PsAA9A boosted cellulolytic activity of glysoside hydrolases from families GH1, GH5, and GH6.This study serves as a starting point towards understanding the functional versatility and biotechnological potential of this enzymatic family, highly represented in wood decay fungi, in Pycnoporus genus. Instituto de Biotecnología Fil: Garrido, Mercedes Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Garrido, Mercedes Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Landoni, Malena. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigación en Hidratos de Carbono; Argentina Fil: Sabbadin, Federico. University of York. Department of Biology. Centre for Novel Agricultural Products (CNAP); Reino Unido Fil: Valacco, Maria Pia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Centro de Estudios Químicos y Biológicos por Espectrometría de Masa; Argentina Fil: Valacco, Maria Pia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Couto, Alicia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigación en Hidratos de Carbono; Argentina Fil: Bruce, Neil Charles. University of York. Department of Biology. Centre for Novel Agricultural Products (CNAP); Reino Unido Fil: Wirth, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina Fil: Wirth, Sonia Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2022-01-13T12:19:56Z 2022-01-13T12:19:56Z 2020-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11114 https://link.springer.com/article/10.1007%2Fs00253-020-10911-6 0175-7598 1432-0614 https://doi.org/10.1007/s00253-020-10911-6 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Applied Microbiology and Biotechnology 104 : 9631–9643. (2020) |
| spellingShingle | Pycnoporus Celulosa Oligosacáridos Biomasa Cellulose Oligosaccharides Biomass cientifico Pycnoporus sanguineus Garrido, Mercedes Maria Landoni, Malena Sabbadin, Federico Valacco, María Pía Couto, Alicia Bruce, Neil Charles Wirth, Sonia Alejandra Campos, Eleonora PsAA9A, a C1-specific AA9 lytic polysaccharide monooxygenase from the white-rot basidiomycete Pycnoporus sanguineus |
| title | PsAA9A, a C1-specific AA9 lytic polysaccharide monooxygenase from the white-rot basidiomycete Pycnoporus sanguineus |
| title_full | PsAA9A, a C1-specific AA9 lytic polysaccharide monooxygenase from the white-rot basidiomycete Pycnoporus sanguineus |
| title_fullStr | PsAA9A, a C1-specific AA9 lytic polysaccharide monooxygenase from the white-rot basidiomycete Pycnoporus sanguineus |
| title_full_unstemmed | PsAA9A, a C1-specific AA9 lytic polysaccharide monooxygenase from the white-rot basidiomycete Pycnoporus sanguineus |
| title_short | PsAA9A, a C1-specific AA9 lytic polysaccharide monooxygenase from the white-rot basidiomycete Pycnoporus sanguineus |
| title_sort | psaa9a a c1 specific aa9 lytic polysaccharide monooxygenase from the white rot basidiomycete pycnoporus sanguineus |
| topic | Pycnoporus Celulosa Oligosacáridos Biomasa Cellulose Oligosaccharides Biomass cientifico Pycnoporus sanguineus |
| url | http://hdl.handle.net/20.500.12123/11114 https://link.springer.com/article/10.1007%2Fs00253-020-10911-6 https://doi.org/10.1007/s00253-020-10911-6 |
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